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Science  07 Jan 2011:
Vol. 331, Issue 6013, pp. 98
DOI: 10.1126/science.331.6013.98-a

Mass Spectrometer

The LCMS-8030 combines the power of triple quadrupole mass spectrometry with unmatched speed to provide the ideal complement to its UHPLC systems. The LCMS-8030 features ultrafast multiple reaction monitoring transitions, enabling data acquisition with up to 500 different channels per second. The improvements to the electronics provide ultrafast mass spectrum measurement speeds of 15,000 μ/sec without sacrificing sensitivity or resolution, and ultrafast polarity switching (15 msec) for the most information without signal deterioration. Patented UFsweeper technology accelerates ions out of the collision cell by forming a pseudo-potential surface. The result is high-efficiency collision-induced dissociation and ultrafast ion transport, reducing the sensitivity losses and cross talk observed on other systems. The LCMS-8030's robust design allows maximum uptime, resulting in a system that can handle most complex matrices. Maintenance of the desolvation line without breaking vacuum minimizes instrument downtime.

Proteoglycan it

Glycosaminoglycans (GAGs) are polysaccharides consisting of repeated dimers. All but hyaluronic acid possess sulfate bases and exist in living organisms in the form of proteoglycans. The new Sulfated GAG Quantitation Kit allows easy quantification of sulfated GAGs in tissues such as cartilage, cultured chondrocytes, and chondrocyte culture solution. Based on a colorimetric determination method using 1,9-dimethylmethylene blue, the new kit is capable of measuring amounts in the 2.5 to 80 μg/mL range within a wide standard curve. Provided in a standard 96-well plate format that can be stored for up to one year, the kit facilitates simple, simultaneous processing of multiple samples. Additionally, a selection of new standards for a broad range of glycobiology and glycoanalysis has been launched, including oversulfated chondroitin sulfate, dermatan sulfate, hyaluronic acid from porcine skin, and heparan sulfate from porcine mucosa.

Glycan/Glycopeptide Data Analysis

SimGlycan is a high throughput glycan and glycopeptide structure elucidation tool, which now supports multi-stage mass spectrometry data analysis which enables identification of structure specific fragment pathways. The program now includes comprehensive support to perform multi-stage/sequential mass spectrometry data analysis, which involves refragmentation of product ions. Multi-stage mass analysis provides structural information that is useful in structure elucidation of metabolites, glycopeptides, and glycans. It aids in discrimination of glycans having similar characteristic fragment ions at MS/MS level. It resolves heterogeneity of carbohydrate distribution and branching pattern of complex glycans such as isobaric isomers.

Protein Extraction

Four new kits that are designed to simplify the extraction of proteins from cells and tissues are now available. Fast and easy to use, individual DUALXtract kits are available for total protein extraction, the extraction of integral membrane proteins and membrane-associated proteins, and the isolation of cytoplasmic and nuclear proteins. DUALXtract Mammalian Cell Total Protein Extraction Reagent provides gentle one-step extraction of total protein, while the DUALXtract Nuclear and Cytoplasmic Protein Extraction Kit rapidly isolates cytoplasmic and nuclear proteins from cultured cells and tissues. The DUALXtract Total Membrane Protein Extraction Kit quickly isolates high quality membrane and cytoplasmic protein fractions from mammalian cells and tissues, with no cross-contamination. The DUALXtract Membrane Protein Optimization Kit can be used to quickly identify optimal extraction conditions for membrane proteins of interest and is suitable for the extraction of native, functional membrane proteins from mammalian, fungal, and bacterial cells.

Protein Digestion Kits

Two new sample preparation kits are designed to advance a "universal" sample preparation protocol for mass spectrometry-based proteome research. Filter-Aided Sample Prep (FASP) Protein Digestion Kits enable complete, reproducible solubilization, and digestion of both fluid and tissue sample proteomes, and extend mass spectrometry-based proteome research methods into the previously inaccessible realms of the insoluble and formalin-fixed paraffin-embedded (FFPE) ("biobank") proteomes. By enabling the use of strong detergents within mass spectrometry sample preparation workflows, the FASP Protein Digestion Kit offers a standardized sample preparation workflow that works for both soluble and insoluble proteins in fluids and tissues. The FFPE-FASP Protein Digestion Kit's composition is similar, but contains supplemental materials and protocol steps to guide researchers through additional tissue sample and protein treatment steps to ensure compatibility of FFPE samples with downstream mass spectrometry sample preparation and analysis workflows.

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  • Newly offered instrumentation, apparatus, and laboratory materials of interest to researchers in all disciplines in academic, industrial, and governmental organizations are featured in this space. Emphasis is given to purpose, chief characteristics, and availabilty of products and materials. Endorsement by Science or AAAS of any products or materials mentioned is not implied. Additional information may be obtained from the manufacturer or supplier.

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