Development

Enucleation Regulation

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Science  21 Jan 2011:
Vol. 331, Issue 6015, pp. 265
DOI: 10.1126/science.331.6015.265-d

Mammalian mature red blood cells lack nuclei. Enucleation results from asymmetric cell division in which the condensed nucleus, surrounded by a plasma membrane, buds off from the immature erythroblast. Micro- (mi) RNAs are important regulators of erythroid cell development and homeostasis; thus Zhang et al. used RNA-seq technology to examine whether they also regulate erythroid maturation. Their analysis revealed that the majority of miRNAs are down-regulated during this process. In particular, expression of miR-191 decreased sharply. Overexpression of miR-191 resulted in defective cell enucleation, but differentiation was unaffected. Reduction of miR-191 expression allowed for the expression of target erythroid genes Riok3 and Mxi1, which are required for chromatin condensation and enucleation.

Genes Dev. 10.1101/gad.1998711 (2010).

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