Technical Comments

Response to Comment on “The Mechanism for Activation of GTP Hydrolysis on the Ribosome”

Science  01 Jul 2011:
Vol. 333, Issue 6038, pp. 37
DOI: 10.1126/science.1202532

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Our report of the crystal structure of elongation factor Tu (EF-Tu) and aminoacyl–transfer RNA bound to the ribosome with a guanosine triphosphate (GTP) analog included a proposed mechanism of GTP hydrolysis by EF-Tu involving histidine-84. Liljas et al. summarize experimental evidence against this mechanism and propose a substrate-assisted catalytic model. However, these experiments and the model are also problematic. Further study is required to definitively determine the mechanism of GTP hydrolysis by EF-Tu.

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