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Science  15 Jul 2011:
Vol. 333, Issue 6040, pp. 365
DOI: 10.1126/science.333.6040.365-a

Multifunction Pyrolyzer

The Multi-Shot Pyrolyzer EGA/PY-3030D is a fourth-generation, multifunctional gas chromatograph (GC) inlet that can chemically characterize most liquids and solids. The new Multi-Shot Pyrolyzer offers a choice of six analytical techniques: Evolved gas analysis (EGA), thermal desorption (TD), reactive pyrolysis (RxPY), single-shot pyrolysis (PY), multi-shot thermal desorption/pyrolysis, and heart cutting (HC-EGA). The Multi-Shot Pyrolyzer is an attractive alternative to solvent-based chemical extraction methods, which take much longer and require reagents. Frontier Pyrolyzers are used to isolate and analyze a wide range of materials including polymers, paints, coatings, inks, fibers, plastics, and woods. A full range of accessories adds flexibility to the Pyrolyzer system. These include a carrier gas selector, an auto-shot sampler that can accommodate up to 48 samples, a selective sampler that can automatically vent sample fractions, a μ-jet cryo trap, a micro-high-pressure reaction sampler, a thermal desorption sampler, a ultraviolet-irradiator, Ultra-ALLOY separation columns, and a vent-free GC/MS adapter.

Western Blotting Transfer System

The Trans-Blot Turbo system is a rapid Western blotting instrument that allows researchers to efficiently transfer proteins from gel to blot in as little as three minutes. Conventional tank and semi-dry Western blotting protocols require time-consuming reagent preparation and setup, followed by an electrophoretic transfer that can take up to an hour or overnight to complete. Researchers using the Trans-Blot Turbo system can overcome this bottleneck by significantly reducing their transfer time to three to seven minutes, depending on protein size. The system uses prepackaged Turbo transfer packs that include paper, buffer, and membrane (nitrocellulose or PVDF), eliminating the time and hassle associated with the preparation of typical Western blots. The Trans-Blot Turbo system delivers better transfer efficiency compared with traditional methods, enabling the detection of proteins larger than 200 kD.

Calcium Flux Assay

The new Primary Sensor assay system is designed for measuring calcium flux in relevant, primary cells. The Primary Sensors are primary human cells that were transiently transfected with the luminescent calcium biosensor i-Photina and subsequently loaded with the biosensor substrate coelenterazine. They are offered cryopreserved, and can be used after reactivation. When stimulating intracellular Ca2+release by activation of endogenously expressed receptors, the potency of potential compounds can be assessed. Primary cells allow for a higher predictability of drug reactions in humans. These cells express relevant drug targets at the physiological level and genuinely carry all the components required for specific signal transduction. They can be derived from the actual tissue of interest. The ready-to-use, cell-based assay system is an excellent tool for studying drug effects on calcium signaling in primary cells.

Chemotaxis Cell Migration

The Millicell μ-Migration Assay Kit is an innovative, slide-based platform that can be used to visualize and measure the effects of chemoattractants on the migration of adherent cells in real time. The kit is ideal for studying metastatic behavior or the effects of toxins. The Boyden chamber is the most commonly used assay for studying chemotaxis. This end-point assay uses chambers separated by a microporous filter that cells migrate through towards a chemoattractant. However, movement of cells through the filter cannot be visualized. The Millicell μ-Migration Assay replaces the traditional filter with microchannels, allowing a cell's migration to be directly observed and its progress to be quantitatively measured. Real-time observation of individual cells provides insight into a number of cell migration parameters, including directionality, velocity, and the migration index of tracked cells. Up to three assays can be run in parallel on each slide with approximately 100 to 200 cells per assay.

Multigas Incubator

The MCO-19M multigas incubator provides extremely accurate control of O2 and CO2 levels combined with precisely regulated temperature. By achieving a stable and uniform culturing environment, the new incubator ensures ideal growth conditions and excellent reproducibility of experimental results. The MCO-19M also introduces the option of a unique hydrogen peroxide decontamination system which enables full decontamination and incubator cleaning to be completed in less than a morning. A sophisticated PID microprocessor provides accurate control of CO2 and O2 levels, ensuring rapid recovery without overshoot after frequent door openings, making it suitable for even the busiest laboratories. The MCO-19M incubator utilizes a patented Direct Heat and Air Jacket conditioning system. With multiple heating zones under microprocessor control, the temperature throughout the chamber is precisely regulated. Users can therefore rely on getting the same results from every shelf. The inCu saFe copper-enriched stainless steel interior continuously inhibits growth of molds, fungi, mycoplasma, and bacteria.

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  • Newly offered instrumentation, apparatus, and laboratory materials of interest to researchers in all disciplines in academic, industrial, and governmental organizations are featured in this space. Emphasis is given to purpose, chief characteristics, and availabilty of products and materials. Endorsement by Science or AAAS of any products or materials mentioned is not implied. Additional information may be obtained from the manufacturer or supplier.

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