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Pre-Clovis Mastodon Hunting 13,800 Years Ago at the Manis Site, Washington

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Science  21 Oct 2011:
Vol. 334, Issue 6054, pp. 351-353
DOI: 10.1126/science.1207663

Abstract

The tip of a projectile point made of mastodon bone is embedded in a rib of a single disarticulated mastodon at the Manis site in the state of Washington. Radiocarbon dating and DNA analysis show that the rib is associated with the other remains and dates to 13,800 years ago. Thus, osseous projectile points, common to the Beringian Upper Paleolithic and Clovis, were made and used during pre-Clovis times in North America. The Manis site, combined with evidence of mammoth hunting at sites in Wisconsin, provides evidence that people were hunting proboscideans at least two millennia before Clovis.

Recent studies have strengthened the case that the makers of Clovis projectile points were not the first people to occupy the Americas (15). If hunting by humans was responsible for the megafauna extinction at the end of the Pleistocene (6), hunting pressures must have begun millennia before Clovis (7). Here we reexamine the evidence from the Manis site in the state of Washington (8), an early mastodon kill that dates to 800 years before Clovis.

Between 1977 and 1979, a single male mastodon (Mammut americanum) was excavated from sediments at the base of a kettle pond at the Manis site (figs. S1 to S3) (810). Some bones were spirally fractured, multiple flakes were removed from one long bone fragment, and other bones showed cut marks (8, 11, 12). The only documented artifact associated with the mastodon was a foreign osseous fragment, interpreted as the tip of a bone or antler projectile point, embedded in a rib fragment that was recovered ex situ from sediments excavated when a backhoe uncovered the bone bed (Fig. 1 and fig. S4) (8). Organic matter associated with the mastodon yielded calibrated radiocarbon ages of ~14 thousand years ago (ka) (8, 10) (table S1). Over the past 35 years, the age and evidence for human involvement with the Manis mastodon have been challenged (13).

Fig. 1

Mastodon rib with the embedded bone projectile point. (A) Closeup view. (B) Reconstruction showing the bone point with the broken tip. The thin layer represents the exterior of the rib. (C) CT x-ray showing the long shaft of the point from the exterior to the interior of the rib. (D) The entire rib fragment with the embedded bone projectile point.

We obtained 13 accelerator mass spectrometry (AMS) 14C dates from purified bone collagen (4) extracted from the mastodon rib containing the embedded osseous object and from both tusks (table S2). All dates were statistically identical at 1 SD and establish an age of 11,960 ± 17 14C years before the present (yr B.P.) for the Manis mastodon (Table 1; average of four XAD fractions; 13,860 to 13,765 calendar yr B.P.) (14). These dates show that the ex situ mastodon rib and in situ skeleton are contemporaneous.

Table 1

AMS 14C ages used to date the Manis Mastodon.

View this table:

High-resolution x-ray computed tomography (CT) scanning (15) revealed that the osseous object embedded in the rib is dense bone shaped to a point (Fig. 1 and movies S1 and S2). The point penetrated 2.15 cm into the rib; the tip broke after entering the rib and separated from the main shaft. The combined length of the point fragment (tip length plus the length of the embedded and external shaft piece) is 3.5 cm.

The rib with the embedded projectile point is a right 12th, 13th, or 14th rib in a series of 19, but most likely the 14th rib (Fig. 2). The projectile point entered the dorsal surface of the proximal end of the rib immediately distal to the lateral margins of the two articular facets at approximately a 45° angle relative to the axis of the head of the rib. The point would have penetrated the hair and skin and about 25 to 30 cm of superficial epaxial muscles (Fig. 2 and fig. S5). Thus it was at least 27 to 32 cm long, comparable with the known length of later, Clovis-age thrown and thrust bone points (16). There is no evidence of bone growth around the point, indicating that the mastodon died soon after it was attacked.

Fig. 2

Anatomical position of the Manis rib. (A) Two vertebrae with the Manis rib inserted into its correct anatomical position. The blue arrow points to the embedded point fragment. (B) Side view of mastodon vertebrae with the Manis rib inserted into its correct anatomical position, with the trajectory of the point indicated. (C) Mastodon skeleton showing the location of ribs 12 to 14.

DNA and protein sequencing were undertaken on the rib and bone point (supporting online material text 4 and 5). Attempts to amplify a 140–base pair (bp) fragment of the 16S mitochondrial DNA (mtDNA) from the rib using universal vertebrate primers (17) produced only modern (human) contamination. However, redesigning primers for a 69-bp fragment (including primers, table S8) of D-loop mtDNA produced sequences from both the rib and bone point that were identical to mastodon and distinct from other proboscideans (mammoth or elephant) by nine substitutions.

We also obtained high-resolution tandem mass spectrometry (MS/MS)–based protein sequences from the projectile point and rib, and used another mastodon sample as a second reference (tables S3 to S6). The MS/MS spectra from the bone point matched the reconstructed mastodon collagen sequences, with the highest scores being within a reference set of collagen sequences (table S7 and supporting table of bone point marker peptides). These results and controls show that the point was fashioned from mastodon bone.

The Manis site provides further evidence of a human presence in the New World 800 years before Clovis [13 ka (4)] and shows that people were hunting with mastodon bone weapons made from earlier kills. Evidence for pre-Clovis hunting also comes from the 14.2-ka Schaefer site and 14.8-ka Hebior site, Wisconsin (18, 19), where stone artifacts, but no projectile points, were found with the remains of mammoth (Mammuthus primigenius). Additional evidence of megafauna hunting comes from sites where artifacts are absent, but taphonomic evidence suggests human butchering, such as at the 13.8-ka Ayer Pond site (45SJ454), Orcas Island, Washington (20). Studies of the dung fungal spore Sporormiella from lakes in Indiana and New York imply that megafauna populations collapsed there between 14.8 and 13.7 ka (7). Thus, the impact of human hunters on the North American megafauna was more prolonged than previously hypothesized and was not a “Clovis blitzkrieg” (21). The absence of stone projectile points at Manis, Hebior, Schaefer, and Orcas Island and the presence of an osseous projectile point at Manis suggest that osseous projectile points may have been the predominant hunting weapon during the pre-Clovis period. Bone and ivory points and other tools are common in the Upper Paleolithic of Siberia and in late Pleistocene sites in Beringia (2224). They are durable and lethal hunting weapons that continued to be used during and after Clovis (16, 23, 25). The invention and spread of a new hunting weapon at 13 ka—the Clovis lithic point—may have accelerated the demise of or doomed the last megafaunal species.

Supporting Online Material

www.sciencemag.org/cgi/content/full/334/6054/351/DC1

SOM Text

Figs. S1 to S5

Tables S1 to S8

References

Table of bone point marker peptides

References and Notes

  1. Acknowledgments: We thank the North Star Archaeological Research Program established by J. Cramer and R. Cramer and the Chair in First Americans Studies for funding. We thank J. Southon for providing the ultrafiltration 14C measurements. Work conducted at the Center for GeoGenetics was supported by the Danish National Research Foundation. E.C. is supported by the European Union with a Marie Curie Intra European Fellowship (grant number 237227). J.O., D.S., and L.J. are supported by the Novo Nordisk Foundation Center for Protein Research. CT scanning was performed at the High Resolution X-ray CT Facility at the University of Texas, Austin. J. Halligan prepared the illustrations. T. Jennings, J. Halligan, T. Goebel, S. Fiedel, and two anonymous individuals reviewed the manuscript.
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