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Science  21 Oct 2011:
Vol. 334, Issue 6054, pp. 395
DOI: 10.1126/science.334.6054.395-a


The CB series incubators provide excellent growth conditions for mammalian cell and tissue cultures, offering precise, reproducible control of oxygen concentrations as low as 0.2% and quick recovery times, important factors in conducting research under hypoxic conditions. Models CB 53, 150, and 210 allow sample access via optional small internal doors that, in addition to maintaining steady CO2 and O2 levels and accelerating recovery, minimize the risk of contamination. The incubators feature as standard a 180°hot-air cycle that provides true sterilization. Interior walls stay dry through controlled humidification with a defined condensation point, and chamber preheating with the VENTAIR air jacket provides homogeneous temperature distribution. A seamless, deep-drawn interior features an integrated shelf-support system for easy cleaning. Single-beam infrared sensor measures and controls CO2 in real-time, independent of other gasses and humidity, and provides stable pH values for optimum, uniform cell growth throughout the entire chamber.

Automated Cell Culture System

The CompacT SC is an automated T-flask cell culture system designed to fully control expansion, differentiation, and maintenance of stem cells. The new CompacT SC, which can be used to aseptically culture multiple different stem cell lines in parallel using T75 and T175 flasks, has a number of features that makes controlling the growth and differentiation of stem cells an easy, convenient task. The system includes automated cell imaging and analysis, allowing scientists to program the CompacT SC to monitor cell morphology and growth curves. It also permits researchers to set the system to respond to any pre-programmed changes by automatically passaging cells and adding new media, reagents, or vectors at appropriate times. This means scientists can consistently maintain cell lines in the state they need them, either as pluripotent cells or during cell differentiation and transfection. Processes can be performed at any time of the day or night, without researchers having to be in the laboratory.

Manual Colony Counter

The aCOLade is an affordable, manual colony counter with automatic result recording, ideal for use in any microbiology laboratory. The new aCOLade colony counter consists of a compact white light box with a pressure controlled back plate and a pen attached to an integrated digital counter. Operating aCOLade is quick and simple because as soon as microbiologists touch the Petri dish's lid with the pen to count a colony, a bleeping sound occurs and the count is automatically added and displayed on the digital counter. To record results, aCOLade can be connected to any computer and comes with software, which allows scientists to automatically transfer their counts to a spreadsheet. This flexible system has a well-designed adjustable plate ring that is used to hold plates as small as 50 mm diameter and can be easily altered to accommodate 90 mm and 120 mm diameter plates, making it suitable for a range of counting applications.

Enzyme Blocking Solution

In immunohistochemical staining, background staining can often be traced to the existence of active endogenous enzyme, such as peroxidase and alkaline phosphatase, in the tissue preparation. This type of interference can generate false positive results or obscure the specific staining of the target antigen. To assist the pathologist, histologists, and other research scientists in obtaining meaningful immunohistochemical data, BLOXALL Blocking Solution has been designed as a dual blocking solution for endogenous alkaline phosphatase and peroxidase in tissues. BLOXALL inactivates all endogenous peroxidase, pseudoperoxidase, and alkaline phosphatase in formalin-fixed, paraffin-embedded tissue sections, frozen tissue sections, and cell preparations. BLOXALL Blocking Solution is a simple, single step, ready-to-use reagent provided in a convenient dropper bottle.

Cell Health/Gene Expression Assay

The ONE-Glo + Tox Assay is designed for improved analysis of reporter gene expression in the context of cell health. The assay combines luciferase chemistry with a cell viability marker in a two-step, addition-only process, to enable measurement of cell viability and gene expression in a single well of a plate, negating the need to run parallel assays. By analyzing reporter activity in relation to cell viability, false results due to toxicity or changes in cell number can be uncovered. The first part of the assay is a non-lytic fluorescence assay (CellTiter-Fluor Cell Viability Assay) that measures the relative number of the live cells in a culture population after experimental manipulation. The second part of the assay uses the ONE-Glo Lucifease Assay System to quantify firefly luciferase reporter gene expression from cells made to express this reporter enzyme. Ideally suited for high and ultrahigh throughput applications, the volumes of each assay component can be scaled to meet throughput needs and is amenable to automation up to 1,536-well format.

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