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Science  04 Nov 2011:
Vol. 334, Issue 6056, pp. 705
DOI: 10.1126/science.334.6056.705-a

RNA/DNA ISOLATION KITS

The MagMAX FFPE Isolation Kits are two new magnetic bead-based kits designed specifically for the isolation of both total RNA and DNA from formalin-fixed paraffin-embedded (FFPE) samples. The MagMAX FFPE kits offer the yield, purity, and downstream performance characteristics that scientists expect, while also delivering faster workflow protocols, higher throughput capacity, and eliminating user-exposure to toxic organic solvents typically associated with the FFPE purification process. To enhance user productivity, MagMAX FFPE Kits incorporate magnetic bead-based technology, yielding a workflow amenable to high throughput processing. Offered in a 96-well format, MagMAX FFPE Kits enable an automated-assist protocol, which can effectively reduce hands-on processing time by 50% or more. By substituting xylene-like solvents with a novel solubilization reagent, MagMAX FFPE kits combine protease enzymes and digestion additives. This combo-reagent allows users to eliminate the deparafinization step of the protocol, and the toxicity associated with traditional FFPE processing.

ChIP-Seq PREPARATION KIT

The Magna ChIP-Seq preparation kit is designed to simplify genome-wide profiling of DNA-protein interactions by chromatin immunoprecipitation and next generation sequencing (ChIP-Seq). In contrast to other products that support only part of the ChIP-Seq workflow, the Magna ChIP-Seq kit is a complete solution that provides a set of validated reagents for both chromatin immunoprecipitation and next generation library construction. These reagents, in combination with a detailed protocol and quality control guidelines, simplify the ChIP-Seq process to allow researchers to map interactions of histones, transcription factors, and other chromatin-associated proteins on a genome-wide scale. As successful immunoprecipitation is critical to construction of the sequencing library, the kit includes positive and negative control antibodies and a set of polymerase chain reaction primers that can be used as in-process controls or to verify technique. The kit has been used to construct ChIP-Seq libraries from as little as 1 ng of purified ChIP DNA, meaning that DNA segments associated with low abundance proteins can be sequenced.

ORGANIC RNA EXTRACTION

The new Direct-zol RNA MiniPrep facilitates efficient and consistent broad range purification of high quality (DNA-free) total RNA directly from samples stored in Tri-Reagent or similar acid-guanidinium-phenol reagents. This innovative procedure bypasses phase separation and precipitation with a spin column and eliminates problems with phenol carryover, all traits typically associated with organic extraction-based methods. The Direct-zol RNA MiniPrep is part of the 'next gen' of RNA prep that will meet the demands of today's scientists requiring RNA that is ideal for stringent analytical methods like miRNA profiling, RNA-seq, and viral identification. The procedure couples the effectiveness of infectious agent inactivation and sample preservation with a convenient no hassle, no-mess procedure for DNA-free RNA.

cDNA SYNTHESIS KIT

The new iScript Advanced cDNA synthesis kit for RT-qPCR allows researchers to generate more real-time polymerase chain reaction (PCR) data from a single 20 μL reverse transcription reaction for their gene expression analyses. With a maximum capacity of 7.5 μg of input RNA, the iScript Advanced cDNA synthesis kit for RT-qPCR surpasses the capacity of its nearest rival by 2 μg. The iScript Advanced cDNA synthesis kit for RT-qPCR offers additional advantages over competitor kits in terms of simplicity and speed. The kit has only two essential components: an iScript Advanced reaction mix and an iScript Advanced reverse transcriptase. The protocol takes only 35 minutes. The iScript Advanced cDNA synthesis kit for RT-qPCR offers excellent sensitivity and efficiency in both real-time PCR (using SYBR Green, EvaGreen, or probes) and conventional RT-PCR.

FISH PROBES

Stellaris FISH (fluorescence in situ hybridization) is an RNA visualization method that allows simultaneous detection, localization, and quantification of individual mRNA molecules at the sub-cellular level in fixed samples. Stellaris FISH probes are manufactured on a custom basis, including software for optimum probe design. In addition, premade probe sets for glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in human and mouse applications are available. This novel RNA FISH technology represents a fast and easy-to-use method to achieve conclusive results through compelling images of RNA expression. By enabling scientists to localize and count discrete molecules of mRNA using widefield fluorescence microscopy, Stellaris FISH probes can lead to streamlined studies in stem cell, cancer, pathology, developmental biology, transcription regulation, and neuroscience research. Stellaris FISH can also be combined with existing technologies such as real-time polymerase chain reaction, DNA FISH, immunohistochemistry, and Western blotting to provide complementary information.

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  • Newly offered instrumentation, apparatus, and laboratory materials of interest to researchers in all disciplines in academic, industrial, and governmental organizations are featured in this space. Emphasis is given to purpose, chief characteristics, and availabilty of products and materials. Endorsement by Science or AAAS of any products or materials mentioned is not implied. Additional information may be obtained from the manufacturer or supplier.

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