Technical Comments

Comment on “Widespread RNA and DNA Sequence Differences in the Human Transcriptome”

Science  16 Mar 2012:
Vol. 335, Issue 6074, pp. 1302
DOI: 10.1126/science.1210484

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Abstract

Li et al. (Research Articles, 1 July 2011, p. 53; published online 19 May 2011) reported more than 10,000 mismatches between messenger RNA and DNA sequences from the same individuals, which they attributed to previously unrecognized mechanisms of gene regulation. We found that at least 88% of these sequence mismatches can likely be explained by technical artifacts such as errors in mapping sequencing reads to a reference genome, sequencing errors, and genetic variation.

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