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Science  11 May 2012:
Vol. 336, Issue 6082, pp. 751
DOI: 10.1126/science.336.6082.751-a

PROTEIN QUANTITATION

The Direct Detect system offers fast, accurate protein quantitation with minimal sample preparation. The infrared (IR)-based spectrometry system and novel membrane technology accurately quantifies amide bonds intrinsic to all proteins, distinguishing proteins from interfering sample components. Because IR-based quantitation does not rely on amino acid composition, dye binding, or redox potential, this approach surpasses conventional UV-Vis spectroscopy and colorimetric assays and allows protein quantification in the presence of reducing agents and detergents. The system requires only a small sample volume (2 μL) and can measure protein concentrations from 0.2 mg/mL to 5 mg/mL within minutes. In addition, the system's calibration standard curve needs to be generated only once, providing additional time savings and ease of use. The Direct Detect system is also capable of quantifying other biomolecules, including lipids and nucleic acids.

RECOMBINANT PROTEIN DETECTION

Detection and purification of recombinant proteins have become even easier with conjugated anti-DDK antibodies. AMSBIO offers a family of anti-tag antibodies to optimize the detection and purification of recombinant tagged proteins. Most of AMSBIO's anti-tag antibodies are mouse monoclonal antibodies which have been screened and extensively validated for high sensitivity and specificity, in applications including Western blot, immunofluorescent staining, flow cytometry, and immunoprecipitation. The anti-DDK clone 4C5 antibody detects the DDK epitope, which is widely used as a tag in recombinant proteins and is included in the pCMV6-Entry vector that forms the backbone of the TrueORF cDNA series. It has been used to validate the expression of over 15,000 mammalian recombinant proteins, purify more than 5,000 human recombinant proteins from HEK293 cells and purify immunogens under native conditions for the development of antibodies. The new conjugated anti-DDK antibodies have been developed using the highest standards of production procedure and validation.

IN VITRO TRANSLATION KITS

Two new kits enable the in vitro expression of full-length proteins from mRNA or DNA templates with yields of up to 750 μg per milliliter of reaction. The expressed proteins by either the Thermo Scientific 1-Step Human Coupled IVT Kit for DNA or the 1-Step Human IVT Kit for RNA can be used to study protein interactions, to perform mutational analysis, or for structural studies. The 1-Step Human IVT Kits rely on the unique HeLa cell lysate that contains all the machinery for in vitro protein expression. While 1-Step Human Coupled IVT reactions are complete in 90 minutes, reactions can be extended for up to six hours for higher protein yields. These kits can produce proteins greater than 250 kD in size and can express functional enzymes, phosphoproteins, glycoproteins, and membrane proteins. In addition to the 1-Step Human IVT Kits, 10 new expression vectors optimized for use with these lysates are now available.

PROTEIN SENSOR CHIP

The ProteOn HTG Sensor Chip provides a robust new alternative for pharmaceutical researchers studying polyhystidine-tagged protein interactions that can reduce cost per data point by 90%. Used with Bio-Rad's ProteOn XPR36 system, the HTG Sensor Chip's innovative surface chemistry has increased binding stability and specificity for His-tagged proteins compared to chips from other surface plasmon resonance (SPR) system providers. Additionally, the HTG Sensor Chip's ability to regenerate and reuse HTG chips multiple times can significantly reduce the cost of generating data. The ProteOn HTG Sensor Chip's Tris-NTA complex contains three NTA molecules for improved binding stability and selectivity to His-tagged molecules. This results in better data quality and reduced ligand decay. Furthermore, binding via the polyhistidine tag is reversible, so the ligand can be removed from the surface and the chip can be reused 10 times or more. This increases reproducibility by eliminating chip-to-chip variability, and it decreases cost per data point.

MONOCLONAL ANTIBODIES

UltraMAB Antibodies are a collection of monoclonal antibodies that are exclusively developed by OriGene for immunohistochemistry (IHC) applications and are the industry's only antibody products available on the market that have been validated with the company's 10K High Density Protein Microarray Technology. OriGene's 10K High Density Protein Microarray Platform enables the screening of candidate antibodies against greater than 10,000 human proteins to identify the most specific monoclonal antibodies for IHC applications. Each UltraMAB is validated for IHC and for immunoassay analysis, and determined to have no cross-reactivity with other human proteins other than the antibody's intended target protein as determined by OriGene's 10K High Density Protein Microarray Platform. Consequently, each UltraMAB is quality control verified, guaranteeing superior performance for IHC applications, where specificity-verification and the highest quality antibodies are critical for accurate detection.

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  • Newly offered instrumentation, apparatus, and laboratory materials of interest to researchers in all disciplines in academic, industrial, and governmental organizations are featured in this space. Emphasis is given to purpose, chief characteristics, and availabilty of products and materials. Endorsement by Science or AAAS of any products or materials mentioned is not implied. Additional information may be obtained from the manufacturer or supplier.

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