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Science  21 Sep 2012:
Vol. 337, Issue 6101, pp. 1557
DOI: 10.1126/science.337.6101.1557-a


The SIGMA HD is a high definition, field emission scanning electron microscope (FE-SEM) that offers high resolution, fast imaging, and easy sample navigation for nanoscale analytics. SIGMA HD incorporates advances in electronics, detectors, and chamber design for imaging resolution as small as one nanometer. It is available in both high-vacuum and variable-pressure modes of operation. Furthermore, a five-axis eucentric stage makes navigation around large samples easy using both translational and tilted movement. SIGMA HD offers unlimited analytical options through diametrically opposite chamber ports that facilitate the mounting of two energy dispersive X-ray spectroscopy detectors for maximum solid angle detection. In the instance of beam sensitive samples, this permits low probe currents to be used while maintaining high X-ray count rates. The favorable geometry afforded by the chamber design eliminates X-ray shadowing effects.


Batch processing in both brightfield and multichannel fluorescence, coupled with user-friendly workflows, makes the SCN400 2.2 scanning platform an all-around high-performance solution for digital pathology scanning. With up to five fluorescence channels per slide, the SCN400 2.2 provides a truly flexible whole-slide scanning solution. Capacity for multiple filter cubes enables up to seven distinct fluorescence channels to be utilized across a scanning batch. Monochrome detectors for fluorescence provide excellent separation of individual fluorophores that may be close in wavelength, while color detectors for brightfield provide excellent digital slide capture quality. For brightfield imaging, the advanced tissue finding system automatically identifies areas for capture, while the Dynamic Focus removes the overhead of slide premapping, rapidly scanning high quality whole-slide images. With the addition of the SL801 autoloader with capacity for 384 slides, the SCN400 2.2 becomes a truly automated high throughput system.


Celigo is a multiapplication cell imaging cytometer able to perform high-content and high throughput fluorescence and brightfield cellular imaging and analysis with minimal sample manipulation. The Celigo is designed for the rapid image capture and processing of cells with minimum plate movements and sample disturbance. Proven with both adherent and nonadherent cell types, its high resolution image acquisition coupled with uniform, high contrast and even illumination allows identification of "every cell in every well." Compatibility with Society for Biomolecular Sciences (SBS) multiwell plates along with T25 and T75 culture flasks, a simplified workflow for all assays, and enhanced software capabilities enable the Celigo to be used in a broad range of applications, including: tumor growth analysis in drug screening, single colony verification, cell migration measurement, automated measurement of cell counting and confluency, and reproducible and reliable embryoid body segmentation.


The JSM-7800F represents a significant leap forward in field emission SEM technology, with unmatched resolution and stability for nanotechnology imaging and analysis. The JSM-7800F uniquely combines an in-lens field emission gun with an aperture angle control lens, optimizing large probe currents (up to 200 nA) for operation at the smallest probe diameter. The new super hybrid lens design and versatile in-column detectors with filtering capabilities allow observation of any specimen, especially at ultralow accelerating voltages down to 10 V. The SEM excels at low accelerating voltage X-ray spectroscopy and cathodoluminescence, combining large beam currents with a small interaction volume and dramatically increasing analytical resolution to the sub-100 nm scale. Beam deceleration in Gentle Beam Mode decreases charging on nonconductive samples and reduces lens aberration effects for extreme high resolution imaging. The JSM-7800F is suitable for a wide variety of applications, from cryomicroscopy to electron beam lithography, and can be configured for low vacuum operation.


Sarstedt introduces new lumox cell culture dishes with ultrathin, gas-permeable film bases. At only 25 μm thick, the lumox film base provides superior optical quality, very low autofluorescence, and high ultraviolet (UV) transparency for microscopy and imaging. Unlike specialty dishes with coverglass bases, the lumox film is gas-permeable for effective gas exchange and homogeneous cell growth. The film has excellent chemical resistance and can be easily cut for cell isolation or storage after fixation and staining. Lumox dishes are available in 35 mm and 50 mm diameters and with surfaces designed for either adherent or suspension cell cultures. The 50 mm lumox dish can be combined with Sarstedt's flexiPERM disc reusable insert for subdivision into four chambers for parallel analyses.

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