Virology

An Atlas of Epstein-Barr Virus

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Science  28 Sep 2012:
Vol. 337, Issue 6102, pp. 1584-1585
DOI: 10.1126/science.337.6102.1584-d

Epstein-Barr virus (EBV), which has been associated with B cell lymphomas, gastic carcinomas, and nasopharyngeal carcinoma, may be responsible for ∼1% of all human cancers. Arvey et al. have pooled data from nucleosome positioning maps and viral protein-binding analyses with more than 700 publicly available high-throughput sequencing data sets from human lymphoblastoid cell lines to generate a large-scale functional genomics atlas of the virus. Although much of the data was already publicly available, it was scattered, and has now been integrated in a highly usable form. Their analysis revealed possible regulatory domains within the viral genome and combinatorial control of viral gene expression by human transcription factors. There were also indications of three-dimensional organization, including loop formation between the viral origin of latent replication and latent membrane proteins 1 and 2, linked by human transcriptional repressor CTCF and cohesin. B cell specificity factor Pax5 was shown to bind to EBV terminal repeats, and depletion experiments showed that Pax5 is involved in the regulation of EBV transcription during latent infection.

Cell Host Microbe 12, 233 (2012).

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