New Products

Science  26 Oct 2012:
Vol. 338, Issue 6106, pp. 549
DOI: 10.1126/science.338.6106.549-a

DNA METHYLATION KIT

The new EZ DNA Methylation-Lightning Kit is for complete bisulfite conversion of DNA prior to methylation analysis by polymerase chain reaction (PCR), MSP, array, or next-gen sequencing. The ready-to-use liquid format Lightning Conversion Reagent is added directly to a DNA sample (as low as 100 pg) for conversion in about an hour. High yield, converted DNA can be eluted into minimal volumes using Zymo's unique spin columns, 96-well spin plates or—a first of its kind—magnetic bead format. This new format enables bisulfite treatment of DNA to be used in conjunction with automated platforms (e.g., Tecan - Freedom EVO) for high throughput processing applications. The EZ DNA Methylation-Lightning Kit is designed to simplify DNA methylation analysis and epigenetic research.

METHYLATED DNA LIBRARY PREP

The NEXTflex Methyl-Seq 1 Kit is designed to enrich and prepare single, paired-end, and multiplexed methylated DNA libraries for sequencing using Illumina MiSeq, GAIIx, and HiSeq platforms while allowing for the multiplexing of up to 96 samples facilitating a methylome-level assessment of genomic DNA. NEXTflex Methyl-Seq Kit utilizes versatile MeDIP or MeCAP protocols for detection of methylated DNA allowing the user to easily assess the methylation state of the genome, quantify absolute DNA methylation levels, and identify differentially methylated regions. The NEXTflex Methyl-Seq 1 Kit includes "Enhanced Adapter Ligation Technology" resulting in library preps with a larger number of unique sequencing reads. This specially designed NEXTflex ligation enzymatic mix allows users to perform ligations with longer adapters and better ligation efficiencies. This kit also uses a completely gel-free protocol making the workflow compatible with liquid handler automation.

SINGLE-STRANDED DNA QUANTITATION SYSTEM

The QuantiFluor ssDNA System is designed for highly sensitive quantitation of single-stranded DNA (ssDNA). The QuantiFluor ssDNA dye enables quantitation of small amounts (as little as 200 pg per well) of ssDNA in solution, saving your valuable sample for downstream assays. For low-concentration samples, the new system delivers sensitivity several thousandfold greater than absorbance at 260 nm and has a higher dynamic range. The QuantiFluor ssDNA System includes all the required reagents to deliver consistent ssDNA quantitation results. It is easy to set up on microplate or single-tube fluorometers and is available as an integrated solution with instrument pairing. Detecting and quantitating ssDNA is important for a variety of molecular biology research applications. These include studying ssDNA viruses, quantitating short synthetic ssDNA probes for site-directed mutagenesis, analyzing first-strand complementary DNAs (cDNAs), and quantitating bisulfate-treated DNA to study DNA methylation.

DNA SAMPLE PREPARATION

PureGenome kits and reagents are designed for rapid and efficient next generation sequencing (NGS) sample preparation. With these reagent sets, library preparation has been streamlined to two steps in under two hours, followed by a short enrichment step, thus alleviating a typical bottleneck in the sequencing process. PureGenome library preparation is a simple, two-step process followed by amplification using EMD Millipore's ultrahigh fidelity KOD Hot Start DNA Polymerase Mastermix. This unique polymerase amplifies DNA with high processivity in highly thymine-adenine (TA)- or guanine-cytosine (GC)-rich regions. The combined efficiency of library construction and accuracy of amplification enables maximum library yields from lower input DNA with minimal bias. The PureGenome NGS library preparation reagents are validated for Illumina platform-compatible NGS libraries; however, end users have the flexibility to optimize for other platforms.

WHOLE GENOME AMPLIFICATION KITS

The new illustra Ready-To-Go GenomiPhi kits provide researchers with a predispensed, room temperature stable formulation for whole genome amplification, enabling a simplified workflow for obtaining large amounts of high-quality DNA from small genomic DNA samples. The new kits also deliver improved yields over the current GenomiPhi kits. Previous GenomiPhi kits contain liquid enzyme formulations that require storage at -80°degrees. The new kits incorporate Ready-To-Go stabilization technology which delivers single-dose reaction mixes in a solid format that can be stored for months at the bench without the need for refrigeration. The new illustra Ready-To-Go GenomiPhi kits are available in two formats: Ready-To-Go GenomiPhi V3, which improves upon the current GenomiPhi V2 kit with more than double the previous DNA yield, and Ready-To-Go GenomiPhi HY, which is specifically developed for high yield requirements, achieving 40 to 60 μg DNA yield from just 10 ng of starting DNA.

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  • Newly offered instrumentation, apparatus, and laboratory materials of interest to researchers in all disciplines in academic, industrial, and governmental organizations are featured in this space. Emphasis is given to purpose, chief characteristics, and availabilty of products and materials. Endorsement by Science or AAAS of any products or materials mentioned is not implied. Additional information may be obtained from the manufacturer or supplier.

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