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Science  30 Nov 2012:
Vol. 338, Issue 6111, pp. 1233
DOI: 10.1126/science.338.6111.1233-a


The new chemiluminescent detection reagent, Clarity Western ECL Substrate, provides excellent results for both high- and low-expression proteins, using either film or digital detection systems. Choosing the right Western blotting detection substrate can be difficult. Ideally, researchers would like to use a single high-performance substrate that works well for all types of blots. In practice, most substrates on the market today are highly specialized. Some substrates work well for the detection of low-expression proteins, whereas others offer less robust performance but are less expensive. The highly versatile Clarity Western ECL Substrate was designed for blots using nitrocellulose, PVFD, and low-fluorescence PVDF and can be used to detect both high- and low-abundance proteins when using film or CCD imaging systems for blot detection. Clarity ECL substrate has a one-year shelf life and is stored at room temperature, allowing the researcher to store it conveniently at hand on the bench.


Two new products have been designed to detect primary antibodies of mouse (WB-2000) or rabbit (WB-1000) origin in Western blot detection: WestVision Peroxidase Polymer Anti-Rabbit IgG and WestVision Peroxidase Polymer Anti-Mouse IgG. The WestVision conjugates are based on a new method of polymerizing enzymes and attaching these polymers to antibodies, allowing a higher density of enzymes to access a target with minimal interference. The result is an excellent signal-to-noise ratio in Western blot detection. WestVision reagents are also economically priced, allowing high sensitivity, low background, and lower cost in Western blot detection. With a chromogenic substrate, the reagents provide enough working solution to typically detect 20 to 200 blots (10 x 10 cm). With a chemiluminescent substrate, dependent on the dilution employed, 800 to 8,000 blots can be detected.


A new antibody is available to detect the oxidized form of calmodulin-dependent protein kinase II (CaMKII), which can be used to characterize and diagnose various diseases. The activation of CaMKII through oxidation is associated with a variety of conditions including heart failure, cardiac arrhythmias, cancer, neurological disease, and sepsis. CaMKII oxidation increases during myocardial ischemia and infarction, and CaMKII oxidation has been shown to play a pivotal role in cardiac cell death during angiotensin II-mediated apoptosis. While previous methods of measuring oxidative stress have relied on "bystander" molecules that are not directly implicated in disease pathogenesis, GeneTex's new antibody against oxidized CaMKII will allow researchers to track the oxidation of a biologically active molecule present in blood or biopsy specimens.


For all scientists requiring highly purified protein, Roche's cOmplete His-Tag Purification Resin is a high-capacity immobilized metal ion affinity chromatography (IMAC) matrix for one-step purification of His-tagged proteins from lysates. In contrast to currently available nitrilotriacetic acid (NTA) resins or resins using chelators, such as iminodiacetic acid (IDA), the nickel ions in this resin are immobilized using one of the strongest chelators known. This new proprietary chemistry minimizes nickel ion leakage, even when using EDTA and DTT containing buffers. This powerful feature, in addition to eliminating the need for resin nickel recharging, also stabilizes purified proteins by preventing nickel ions from catalyzing protein oxidation.


Three new kinase assays, all based on the powerful HTRF technology, were designed to provide new, easy, and rapid tools for the study of different signaling pathways in drug discovery research, from high throughput screening to lead optimization phases. The assays, Phospho-STAT3 (Signal Transducer and Activator of Transcription 3) (Tyr705), Phospho-p38 MAPK (Thr180/Tyr182), and Phospho-IKKβ (Ser177/Ser181) involve targets that are associated with many diseases, including cancer, rheumatoid arthritis, Crohn's disease, pulmonary fibrosis, and acute lung injury. The three new cellular kinase assays are homogeneous, nonradioactive, and nonbead based. Their mix and read protocol eliminates the need for washing steps and their long signal stability and flexibility allow for multiple experiments—including small series—without requiring special equipment, saving valuable time and cost.

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