New Products

Science  07 Dec 2012:
Vol. 338, Issue 6112, pp. 1369
DOI: 10.1126/science.338.6112.1369-a

3-D HANGING DROP PLATES

The new Perfecta3D Hanging Drop Plates allow researchers to form and test reproducible 3-D cell cultures in a 96-well format. The unique design of the Perfecta3D Hanging Drop Plates simplifies and streamlines spheroid formation, culture, and subsequent testing of the 3-D cellular constructs without the aid of coatings or matrices. Spheroid cultures grown in Perfecta3D Hanging Drop Plates allow researchers to easily mimic tissue metabolic and proliferative gradients, capture complex cell-matrix and cell-cell interactions, conduct co-cultures, and monitor cell growth easily and regularly. Media and compounds can be added or removed from the top of the plate without requiring new equipment, and spheroids can be harvested for analysis. Users simply pipet a cell suspension into each channel; the channel shape causes the drop to securely hang from the bottom of the well. One spheroid forms per well, and the spheroid diameter is controlled by the cell type and number of cells added to each well.

3-D CULTURE CELL HARVESTING KIT

3-D cultures exhibit cellular behaviors and morphologies similar to those seen in vivo; however, the adaptation of 3-D culture models for studying biochemical processes has been impeded by the challenge of separating intact or 3-D grown cells from extracellular proteins comprising the 3-D matrix. The Cultrex 3D Culture Cell Harvesting Kit is based on a nonenzymatic approach that prevents biochemical degradation during processing and overcomes the problems associated with extracting cells from 3-D culture in BME or Laminin I. The quality of the harvested cells is ideal for Western blotting and RNA and DNA analysis. This new kit provides an optimized and standardized solution for the isolation and normalization of cell lysates from 3D Culture Matrix, BME, or Laminin I for subsequent biochemical analysis.

CELL CULTURE PLATFORM

The Cytoo 2D+ Cell Culture Platform is based on the of use micropatterns on plates to drive/control the behavior and localization of cells in cultures. Modifying cell shape can be a very efficient way to modify cell functions, including cell fate and differentiation. Cells are exquisite mechano-sensing systems and adapt permanently to their boundary conditions. Thus, contrary to the frequent opinion that classical 2-D culture on infinite adhesive substrates are more representative of cell behavior (as cells are 'free' to spread and move), these conditions introduce a considerable, and unnoticed, variability in cell functions. 2D+ Technology controls the 3-D shape of cultured cells by controlling the 2-D topology of cell adhesion and setting the cell shape in the z axis. Since each micropattern is identical (several hundred per well), cells perform in a reproducible and consistent manner. Thus, fewer precious primary cells and less replicates per data point are needed.

3-D CELLULAR IMAGING SOFTWARE

Volocity 6.0 is a 3-D cellular imaging software featuring a suite of high-performance tools for exploring, interacting, and publishing 3-D imaging data from cells, tissues, and organisms and how they behave over time. The software is designed as a universal solution that provides users with significant functionality to visualize, analyze, and validate 3-D fluorescence images from a wide range of confocal microscopy, wide-field, and high-content screening systems, and is fully integrated for a seamless user experience. Beyond simple cells, the Volocity software can also organize and relate measurements according to biological classification such as nuclei, membranes, organelles, and proteins, making it easier and faster to perform an analysis and understand the results. The updated interface also enables users of all skill levels to perform complex and challenging biological measurements, thereby extending the power of 3-D analysis to a wider population of potential users.

STEM CELL CULTURE MEDIUM

Stemline Pluripotent Culture Medium is a novel human pluripotent stem cell culture medium that provides a consistent environment for the long-term maintenance and growth of healthy pluripotent stem cells. Stemline Pluripotent Culture Medium performs equivalently to the leading medium for maintaining pluripotency and optimal growth rates and is produced more efficiently than traditional media, resulting in lower costs. Culturing pluripotent stem cells can be challenging as many media's undefined, heterogenous mixtures can cause inconsistent growth rates and undesired spontaneous differentiation. The Stemline Pluripotent Stem Cell Culture Medium is serum-free, composed of fully defined components and has 80% less basic fibroblast growth factor than the leading pluripotent stem cell culture medium. This provides a consistent environment for long-term maintenance of optimal growth rates, viability, and pluripotency.

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