New Products

Science  04 Jan 2013:
Vol. 339, Issue 6115, pp. 99
DOI: 10.1126/science.339.6115.99-a

LABEL-FREE PROTEIN ANALYSIS

BLItz is a revolutionary new platform that will enable scientists—for the first time—to conduct label-free analyses of proteins using small sample volumes easily and cost-efficiently at their lab bench. With BLItz, researchers will be able to perform specific protein quantitation, kinetics, and affinity analyses of protein interactions with just a drop of sample in a simple 'drop, read, done' format. BLItz is small—taking up less surface area than a tablet personal computer—so it can fit easily in an individual scientist's workspace and is simple to use. It requires only 4 μL of sample—15 to 20 times less volume than needed for traditional, SPR-based label-free assays or microplate-based ELISA assays. Additionally, its Dip and Read assays utilize disposable, ready-to-use biosensors to uniquely provide results in seconds to minutes. BLItz utilizes BioLayer Interferometry (BLI) technology, enabling real-time kinetics, affinity, and quantitation measurements in high throughput applications.

PROTEIN EXPRESSION REPORTER KITS

A new suite of X-MAN Reporter Kits has been designed for investigation of protein expression levels, protein localization within a cell, and protein-protein interaction within a molecular pathway, at natural levels of protein and promoter activity. The kits include genetically defined reporter cell lines generated using Horizon's GENESIS gene editing technology with Promega's luminescent and fluorescent reporter technologies. X-MAN NanoLuc Reporter cell lines incorporate Promega's new NanoLuc Luciferase reporter directly into the native loci of genes of interest, permitting evaluation of genes and proteins at physiologically relevant levels. X-MAN HaloTag Reporter cell lines facilitate the introduction of Promega's HaloTag as an N- or C-terminal fusion to genes encoding proteins of interest at their native genetic loci. X-MAN Glo Reporter Kits include X-MAN cell lines pretransfected with Promega's Firefly Luciferase for in vivo imaging and VivoGlo luciferin, provided as isogenic cell lines for experimental control.

MONOCLONAL ANTIBODIES

Fourteen new human monoclonal antibodies from Trillium Diagnostics have been added to Beckman Coulter's flow cytometry portfolio. The analyte specific reagents (ASRs) provide high specificity, convenience, and flexibility in flow cytometry. A new cell permeabilization reagent, Intra-Cell, is also being offered. The new monoclonals include human anti-hemoglobin F, which recognizes fetal hemoglobin molecules; CD163 markers for anti-inflammatory signal and disease research; and CD64 markers, which react with Fc gamma 1 receptors. Characterized, quality controlled, convenient, liquid reagents allow users to spend less time in sample preparation and more time on experiments. Rounding out the line is a monoclonal for myeloid nuclear differentiation antigen (MNDA). All antibodies are available unconjugated or conjugated to a selection of fluorochromes. The new antibodies are supplied in 1.0 mL of buffer, are ready to use, and facilitate flexibility in experimental design.

PROTEIN DETECTION TEST

ProReveal Test is a rapid in situ fluorescence-based method of detecting proteins on surgical instruments. The patented, ultrasensitive ProReveal Test combines an integrated ProReveal Protein Test Kit and a ProReveal Viewer with easy-to-use touchscreen and software. In just two simple spray and see steps, users can detect nanogram levels of protein contamination, no matter where it is located on their entire surgical instrument, in less than five minutes. The ProReveal Test aligns with the protein contamination testing recommendations in the United Kingdom's new NHS CFPP-01-01 consultation document. The ProReveal Test uses very sensitive, yet cost-effective, fluorescence technology that will tell you exactly where and how much protein is on your surgical instrument—a significant quality enhancement from the current testing available.

IMMUNOAFFINITY SAMPLE PREPARATION

Mass Spectrometric Immunoassay (MSIA) pipette tip technology delivers a simplified way to enrich target proteins for downstream mass spectrometric analysis. The new MSIA pipette tips provide scientists with an easier immunoaffinity approach that yields better and more reproducible qualified results. MSIA tips feature a 300 μL pipette tip embedded with a proprietary, highly porous immunoaffinity column that provides increased sensitivity with improved reproducibility and sample throughput. Benefits include: enhanced antibody/antigen-binding kinetics that allow enrichment down to very low levels (femtomoles) for downstream mass spectrometric protein/peptide quantification; improved signal-to-noise ratios due to increased specificity and target recovery as compared to conventional bead-based methods; fast and flexible sample throughput with manual multichannel or fully automated 96-well processing in less than 30 minutes; and customizable assays that meet different biomarker research needs.

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  • Newly offered instrumentation, apparatus, and laboratory materials of interest to researchers in all disciplines in academic, industrial, and governmental organizations are featured in this space. Emphasis is given to purpose, chief characteristics, and availabilty of products and materials. Endorsement by Science or AAAS of any products or materials mentioned is not implied. Additional information may be obtained from the manufacturer or supplier.

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