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Influence of Threonine Metabolism on S-Adenosylmethionine and Histone Methylation

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Science  11 Jan 2013:
Vol. 339, Issue 6116, pp. 222-226
DOI: 10.1126/science.1226603

SAM, Histones, and Stem Cells

Mouse embryonic stem cells require threonine for growth and express large amounts of the enzyme that catalyzes the first step in threonine metabolism. To find out what is so important about threonine in these cells, Shyh-Change et al. (p. 222, published online 1 November; see the Perspective by Sassone-Corsi) monitored changes in metabolism by mass spectrometry in induced pluripotent stem cells. Threonine was required to maintain cellular concentrations of S-adenosylmethionine (SAM), a substrate used for protein methylation. Restriction of threonine inhibited methylation of histones, which is characteristic of chromatin in embryonic stem cells. Thus, altered metabolism of threonine and methionine in stem cells may be linked to epigenetic changes that influence genetic reprogramming and decisions of stem cells to proliferate or differentiate.

Abstract

Threonine is the only amino acid critically required for the pluripotency of mouse embryonic stem cells (mESCs), but the detailed mechanism remains unclear. We found that threonine and S-adenosylmethionine (SAM) metabolism are coupled in pluripotent stem cells, resulting in regulation of histone methylation. Isotope labeling of mESCs revealed that threonine provides a substantial fraction of both the cellular glycine and the acetyl–coenzyme A (CoA) needed for SAM synthesis. Depletion of threonine from the culture medium or threonine dehydrogenase (Tdh) from mESCs decreased accumulation of SAM and decreased trimethylation of histone H3 lysine 4 (H3K4me3), leading to slowed growth and increased differentiation. Thus, abundance of SAM appears to influence H3K4me3, providing a possible mechanism by which modulation of a metabolic pathway might influence stem cell fate.

Connections between pluripotency and the metabolic state of mouse embryonic stem cells (mESCs) are only beginning to be described (1). Metabolically, mESCs are characterized by a distinct mode of amino acid catabolism driven by the enzyme that catalyzes threonine (Thr) oxidation, threonine dehydrogenase (Tdh) (1). The abundance of Tdh in mESCs is more than 200 times that in mouse embryonic fibroblasts (MEFs), and Thr restriction or Tdh inhibition abolishes mESC growth (13). Thus, Thr oxidation appears to be critical for mESCs, but the relationship between downstream metabolites and the pluripotent state remains unclear.

To investigate how metabolism is altered upon reprogramming to the pluripotent state, we used liquid chromatography–based tandem mass spectrometry (LC-MS/MS) (4) to profile metabolomic changes during reprogramming into induced pluripotent stem cells (iPSCs). We used MEFs transfected with doxycycline-activated transgenes encoding Oct4, Sox2, Klf4, and Myc (iOSKM) (2, 5) and profiled metabolism during reprogramming. Hierarchical clustering based on the abundance of each metabolite revealed that iPSCs were similar to mESCs and were distinct from MEFs (Fig. 1, A and B). Inspection of the top metabolites regulated by OSKM-induced reprogramming revealed a large number of glycolytic intermediates (fig. S1A and table S1), many of which were significantly more abundant within just 4 days of reprogramming (Fig. 1C), when proliferation was accelerated but the cells were not yet pluripotent (2). Thus, increases in glycolytic intermediates occurred before the acquisition of pluripotency (Fig. 1C). The other top metabolites regulated during reprogramming are involved in Thr and S-adenosylmethionine (SAM) metabolism. In contrast to glycolytic intermediates, Thr- and SAM-related metabolites changed late in reprogramming, which suggests that enhanced Thr and SAM metabolism accompanied acquisition of the pluripotent state (Fig. 1D). Upon reprogramming, some of the largest decreases were in Thr, Cys, and folate, and the largest increases were in SAM and cystathionine.

Fig. 1

The Thr-SAM pathway is activated by pluripotency factors. (A) Heat map showing relative abundance of metabolites in iOSKM-MEFs in the absence of doxycycline treatment (no dox); 4 days after dox (dox 4D); 18 days after dox, whereupon iPSC clones are fully reprogrammed (dox 18D); and mESCs, normalized to total biomass (n = 3), and measured using selected reaction monitoring (SRM) and LC-MS/MS. (B) Scatterplot of metabolite intensities in mESCs (x axis) versus iPSCs (y axis). Metabolite intensities were obtained from the integrated total ion current from a single SRM transition. (C) SRM analysis of abundance in glycolytic intermediates in iOSKM-MEFs during reprogramming (n = 3). *P < 0.05, **P < 0.01. (D) SRM analysis of abundance in Thr-SAM pathway intermediates in iOSKM-MEFs during reprogramming (n = 3). **P < 0.01. (E) SRM analysis of abundance in Thr-SAM pathway intermediates in iLet-7 and iLin28a mESCs after dox (n = 3). All error bars represent SEM from three independent measurements.

Metabolism is also rewired during mESC differentiation. In mESCs, the let-7 microRNA promotes differentiation by suppressing the pluripotency network, whereas Lin28a promotes pluripotency by repressing let-7 (6, 7). Hence, we profiled metabolic changes in mESCs acutely after dox-induced activation of a transgene encoding let-7 or Lin28a (iLet-7 or iLin28a) while the cell cycle remained unperturbed (fig. S1B). Lin28a and let-7 regulated 38 metabolites in a reciprocal manner (fig. S1C), among which were a large number of Thr and SAM metabolites (Fig. 1E); these findings provide further support for the idea that Thr and SAM metabolism are coupled to pluripotency.

We integrated our metabolomics data on mESCs (table S1) with cDNA microarray data on mESCs (2) using the KEGG database of metabolic networks. This analysis showed that many of the metabolic enzymes that channel Thr metabolism into SAM metabolism (e.g., Tdh, Gcat, Gldc, Dhfr, Fol1r, Mat2a/b, and Ahcy) are more abundant in mESCs than in MEFs (Fig. 2A). Consistent with the enzyme expression patterns, several Thr-SAM pathway inputs such as Thr, Cys, and folate were less abundant in mESCs than in MEFs, whereas downstream outputs such as SAM and cystathionine were more abundant in mESCs than in MEFs (Fig. 1D). Thus, a Thr-SAM pathway appears to be activated in mESCs.

Fig. 2

Thr is catabolized to maintain the SAM/SAH ratio in mESCs. (A) Schematic of Thr-SAM pathway activity in pluripotent stem cells, relative to MEFs. (B) HPLC analysis of 14C-labeled amino acids derived from [U-14C]Thr in mESCs after 24 hours. Scintillation counts per minute (CPM) are plotted against retention time. (C) Fraction of intracellular metabolites derived from [U-13C]Thr in mESCs over 5 hours, as measured by SRM analysis (n = 3). (D) Steady-state fraction of intracellular metabolites derived from [U-13C]Thr, [U-13C]Ser, [U-13C]glucose, or [U-13C]Gln in mESCs after 48 hours, as measured by SRM analysis (n = 3). (E) SRM analysis of metabolite abundances in mESCs during 6 hours of Thr restriction, relative to time zero (n = 3). (F) SRM analysis of several metabolic ratios over a 6-hour time course, relative to time zero (n = 3). (G) Feeder-free mESCs were subjected to Thr restriction for 12 hours, then supplemented for 36 hours with the indicated metabolites at the given concentrations. Alkaline phosphatase–positive colonies were quantified and normalized to mESC colony numbers in normal mESC media (% colonies recovered). X denotes relative concentration with respect to DMEM. NAC, N-acetylcysteine; Py, pyruvate; DMG, dimethylglycine; Bet, betaine; H, hypoxanthine; T, thymidine. All error bars represent SEM from three independent measurements.

To test this pathway, we traced the metabolic fate of [U-14C]Thr in mESCs with high-performance liquid chromatography (HPLC). 14C was incorporated into Gly and Glu, indicating that Thr was used to synthesize these amino acids (Fig. 2B). In contrast, MEFs incubated with [U-14C]Thr did not exhibit Thr catabolism (fig. S2A). We also traced the fate of [U-13C]Thr in mESCs with LC-MS/MS metabolomics (fig. S2, B to F, and table S1). mESCs used Thr to synthesize acetyl-CoA–derived tricarboxylic acid (TCA) cycle intermediates (Fig. 2, C and D). At steady state, [U-13C]Thr contributed ~20% of the citrate via acetyl-CoA, whereas [U-13C]glucose contributed ~35% via acetyl-CoA (+2 isotopomer). Thus, Thr contributes significantly to the acetyl-CoA pool in mESCs (Fig. 2D). [U-13C]Thr-derived Gly also donated its 13C-methyl group to ultimately generate 5-methyltetrahydrofolate (5mTHF) and SAM (+1 isotopomer), whereas [U-13C]Ser-derived Gly contributed little to the synthesis of these metabolites (Fig. 2, C and D). Although only ~25% of intracellular Gly and 5mTHF, and ~10% of SAM, were labeled by [U-13C]Thr at steady state, these numbers underestimate the contribution of Thr to the synthesis of these intermediates, because of rapid one-for-one antiport of intracellular [13C]Gly and [13C]Met for extracellular [12C]Gly and [12C]Met. Evidence for rapid antiport-mediated 13C dilution was provided by the appearance of large quantities of [13C]Gly and [13C]Met in the media: Within 1 hour of [13C]Thr addition to the media, 6% of extracellular Gly and 13% of extracellular Met was 13C-labeled, with no substantial consumption of Gly or Met from the media (fig. S2G). These data suggest that the rapid conversion of Thr to Gly by Tdh provides a major fraction of the 5mTHF needed for recycling S-adenosylhomocysteine (SAH) to SAM.

To test whether Thr was indeed a major fuel source for Gly and SAM metabolism, we profiled metabolic changes upon Thr restriction in mESCs; all the Thr in Dulbecco’s modified Eagle’s medium (DMEM) was removed, but there remained enough Thr in the serum for cellular protein synthesis (1, 8). Thr restriction affected mESC viability additively with, and thus independently of, protein synthesis (fig. S2, H and I). The 80% drop in intracellular Thr caused intracellular Gly and the SAM/SAH ratio to decrease steadily, suggesting an imbalance in Gly synthesis and consumption (Fig. 2, E and F, and table S1). In contrast, glucose-6-phosphate, fructose-6-phosphate, lactate, and the ATP/AMP (adenosine triphosphate/adenosine monophosphate) ratio increased (Fig. 2, E and F), consistent with a compensatory increase in glycolysis. However, some of the TCA cycle intermediates still decreased (fig. S2J). The drop in NADH (reduced nicotinamide adenine dinucleotide) might indicate a drop in Thr-derived acetyl-CoA, which fuels the TCA cycle’s production of reducing equivalents (Fig. 2F and fig. S2, K and L). Thus, our results support the idea that Thr is critically required in mESCs because of its contribution to Gly for 5mTHF and SAM synthesis, and because acetyl-CoA produced from Thr could contribute to this anabolic process (Fig. 2A).

As another test of this model, we attempted to suppress the effects of Thr restriction on mESCs by supplementing the culture medium with downstream metabolites in the Thr-SAM pathway. Addition of Gly and pyruvate (a source of acetyl-CoA), but neither alone, prevented mESC death after Thr restriction (Fig. 2G). Glucose, acetate, Ser, Cys, Glu, Gln, N-acetylcysteine, ascorbate, or combinations with pyruvate all failed to prevent mESC death after Thr restriction. However, a combination of pyruvate with the methyl donors dimethylglycine or betaine prevented death from Thr restriction (Fig. 2G); these results suggest that Thr-derived 5mTHF and its main product SAM (Fig. 2, C and D) are essential for mESCs. SAM could not be used because it cannot cross mammalian plasma membranes (9). In contrast, 3-deazaadenosine (DZA) (10), which decreases the SAM/SAH ratio by inhibiting SAH hydrolase, did enter mESCs and inhibited mESC viability (fig. S2M). Hypoxanthine and thymidine also failed to prevent mESC death after Thr restriction although they could enter mESCs (11), which suggests that nucleosides are not important downstream products of Thr-derived Gly. A downstream product of SAM catabolism, the polyamine spermidine (12), was also ineffective. Thus, Thr appears to be necessary to generate the optimal balance of Gly and acetyl-CoA required to synthesize 5mTHF for maintaining the SAM/SAH ratio in mESCs.

SAM is the universal substrate for all protein methylation reactions in the cell, and the SAM/SAH ratio is important for regulating protein methylation because methyltransferases are product-inhibited by SAH (Fig. 3A) (13). To test whether Thr restriction affects protein methylation, we used a pan–methyl-lysine antibody to detect differential lysine methylation of proteins after restriction of Thr, Gly, and Ser. Among the most abundant methylated proteins (14), histone H3 showed a drop in methylation upon Thr restriction, whereas heat shock protein 8, elongation factor-1a, and actin showed only subtle changes (Fig. 3B). Because euchromatin is crucial for epigenetic plasticity in mESCs, and because histone H3 lysine 4 trimethylation (H3K4me3) and H3 acetylation (H3ac) are important for maintaining euchromatin (1518), we tested whether Thr catabolism regulates H3K4me3 and H3ac in mESCs. Under milder conditions of Thr restriction (0.3X) that did not inhibit mESC growth (fig. S3A), H3K4me3 dropped after 48 hours, whereas H3ac remained unchanged (Fig. 3C). In comparison, MEFs showed no changes in H3K4me3 nor H3ac upon 0.3X Thr restriction (Fig. 3C), consistent with our observations that Thr-SAM metabolism is activated only in the pluripotent state.

Fig. 3

Influence of Thr-SAM metabolism on H3K4 methylation. (A) Schematic of methyltransferase reactions. (B) Immunoblot analysis of proteins from mESCs with an antibody against methyl-lysine, after restriction of the indicated amino acids for 24 hours. (C) Immunoblot analysis of mESCs and MEFs for H3K4me3 and H3ac when exposed to 3X and 0.3X concentrations for 48 hours. (D) Immunoblot analysis of mESCs for H3K4me3 after Thr restriction (0X) for 6 hours, then re-fed for 6 hours with indicated metabolites. (E) SAM/SAH ratio after Thr restriction (0X) for 6 hours, then re-fed for 6 hours with indicated metabolites. (F) Immunoblot analysis of mESCs for H3K4me3, H3K4me2, H3K4me1, H3K9me3, H3K27me3, H3K36me3, and H3K79me3 after restriction (0X) of the indicated amino acids for 24 hours.

To test the sensitivity and reversibility of H3K4me3, we supplemented Thr-restricted mESCs with various doses of Thr. Although mESCs lost most of their H3K4me3 after just 6 hours of Thr restriction, refeeding with various concentrations of Thr for 6 hours reversed the drop in H3K4me3 (fig. S3B). H3K4me3 could also be restored by adding pyruvate and Gly to the medium (Fig. 3D). Both pyruvate and Gly were necessary to restore the SAM/SAH ratio to a normal state, consistent with the idea that Thr-derived acetyl-CoA and Gly regulate H3K4me3 by modulating the SAM/SAH ratio (Fig. 3E). Conversely, DZA, which decreases the SAM/SAH ratio, led to a rapid extinction of H3K4me3 and overrode the effects of pyruvate and Gly on mESCs (Fig. 3, D and E, and fig. S3C), which suggests that acetyl-CoA and Gly regulate the SAM/SAH ratio to modulate H3K4me3. We then tested H3 methylation on lysines 4, 9, 27, 36, and 79 in mESCs after restriction of a variety of individual amino acids. H3K4me3 and H3K4me2 were decreased by Thr restriction. H3K4me1, H3K9me3, H3K27me3, H3K36me3, and H3K79me3 did not change significantly with any amino acid (Fig. 3F). Also, α-ketoglutarate did not change significantly during Thr restriction (fig. S2J), suggesting minimal influence on α-ketoglutarate–dependent histone demethylases. These results suggest that the SAM/SAH ratio maintained by Thr catabolism is not required to regulate all protein lysine methylation, but rather certain specific lysines such as H3K4me2 and H3K4me3 in mESCs (Fig. 3F). Assays of total H3K4 methyltransferase activity from nuclear lysates further indicated that the change in H3K4me3 with Thr concentration was not due to a change in the net amount of methyltransferase activity (fig. S3D), supporting the idea that it is the change in the substrate/product ratio (SAM/SAH) that influenced the amount of H3K4me3.

To test whether the Thr-SAM pathway has functional consequences on the pluripotency and differentiation of mESCs, we partially depleted the Tdh enzyme by RNA interference (Fig. 4A and fig. S4A). In low-Thr conditions, partial Tdh depletion led to a decrease in mESC growth and an increase in mESC differentiation (Fig. 4B). Expression profiling by quantitative reverse transcription polymerase chain reaction (qRT-PCR) revealed that Tdh depletion led to a decrease in expression of pluripotency factors, including Oct4, Sox2, Nanog, Rex1, and Blimp1, and increased expression of the differentiation factors Foxa2 and Sox17 (fig. S4B). Embryoid body differentiation of the mESCs for 3 days showed that Tdh depletion led to more rapid extinction of the pluripotency factor Sox2 and an aberrant increase in transcription of the differentiation factors Gata4 and Sox17 (fig. S4C).

Fig. 4

Tdh regulates the pluripotency of mESCs. (A) Immunoblot for Tdh, H3K4me3, H3K27me3, and pan–methyl-lysine in mESCs grown under 0.1X Thr, immediately after depletion of Tdh with two different short hairpin RNAs (shTdh), compared to a control shRNA targeting luciferase (shLuc). (B) Micrographs of alkaline phosphatase staining in mESCs after shTdh or shLuc, seeded at clonal density without feeder MEFs, after 48 hours of culture in 0.1X, 0.3X, and 1X Thr concentrations. (C) Steady-state fraction of intracellular metabolites derived from [U-13C]Thr in mESCs after 24 hours, as measured by SRM analysis, after 48 hours of dox induction of shTdh or shLuc (n = 3). (D) SRM analysis of metabolite abundances in mESCs after 48 hours of dox induction of shTdh or shLuc (n = 3). All error bars represent SEM from three independent measurements.

The differentiation of mESCs after partial Tdh depletion was dependent on the amount of Thr in the culture medium, which suggests that Tdh was required for its metabolic function (Fig. 4B). Indeed, Tdh depletion decreased Thr-SAM flux (Fig. 4C). At steady state, Tdh depletion also decreased Gly, citrate, and the SAM/SAH ratio (Fig. 4D). Tdh depletion also decreased H3K4me3 abundance (Fig. 4A). Thus, Thr catabolism by Tdh appears to be required for maintenance of the pluripotent epigenetic state. We also tested whether enzymes downstream of Tdh in the Thr-SAM pathway are also required by mESCs, including glycine decarboxylase (Gldc), which produces methylene-THF from Gly (19), and methionine adenosyltransferase (Mat2a), which produces SAM from Met. Depletion of Gldc decreased Thr-SAM flux and the level of H3K4me3, and decreased mESC colony growth (fig. S4, D to H). In contrast, transient overexpression of Gldc prevented mESC death after Thr restriction (fig. S4, I and J), suggesting that the 5mTHF and SAM downstream of Thr and Gly are necessary for mESCs. Depletion of the downstream Mat2a also decreased SAM synthesis and H3K4me3 levels, and decreased mESC colony growth (fig. S4, F to H). Conversely, transient overexpression of the SAH hydrolase (Ahcy), which decreases SAH and thereby increases the SAM/SAH ratio, prevented mESC death after Thr restriction (fig. S4, I and J). Finally, components of the H3K4 methyltransferase complexes—such as Wdr5, Dpy30, and Setd1a—also improved mESC survival after Thr restriction, supporting the idea that Thr-SAM metabolism may affect mESCs in part through effects on H3K4me3.

Our results show that the Thr-SAM pathway is activated in mouse pluripotent stem cells. Thr-dependent changes in the SAM/SAH ratio correlated with H3K4me3, thus revealing a possible mechanistic link between cellular metabolism and the epigenetic state. The unique activity of Tdh in converting Thr into both Gly and acetyl-CoA appears to optimize the synthesis of SAM to maintain a high SAM/SAH ratio. H3K4me3 was also more sensitive than methylation of other histone H3 lysines to changes in Thr metabolism, possibly because of the high abundance of this H3 methylation mark in the euchromatin of mESCs and its rapid turnover (1518, 20, 21). H3K4me3 is critical for self-renewal of pluripotent stem cells (22). Because dysregulation of Gly metabolism has been implicated in a variety of human cancers (4, 19, 23, 24) and because the upstream TDH enzyme has been lost by mutation in humans (1), our findings may provide insight into a metabolic pathway that is dysregulated during human tumorigenesis.

Supplementary Materials

References and Notes

  1. Acknowledgments: We thank members of the Daley and Cantley labs for helpful discussions. Supported by the NSS Scholarship from the Agency for Science, Technology and Research, Singapore (N.S.-C.), the American Cancer Society and NIH grant 4R00CA168997-02 (J.W.L.), a Damon Runyon Cancer Research Foundation Amgen fellowship (C.A.L.), NIH grants RC2HL102815 and U01 HL100001 (G.Q.D.), and NIH grant R01 GM56203 (L.C.C.). G.Q.D. is an investigator of the HHMI. G.Q.D., as a co-founder and scientific advisory board member, holds stock options and receives consulting fees from iPierian Inc., a biopharmaceutical company that uses iPS cells in drug discovery against neurologic disease. L.C.C. is the founder and a member of the board of directors of Agios Pharmaceuticals, a company that targets metabolic enzymes for cancer treatment.
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