New Products

Science  01 Mar 2013:
Vol. 339, Issue 6123, pp. 1103
DOI: 10.1126/science.339.6123.1103-a

TRANSILLUMINATORS

New transilluminators with high-performance blue light LEDs are available for gel documentation purposes. Blue light transilluminators are an interesting alternative to ultraviolet (UV) tables as there is no risk of sample damage during illumination, which is important for processing samples following gel documentation. The transilluminators also benefit users, as there is no risk of UV exposure. Blue light excitation can be used with fluorescent dyes for nucleic acid or for protein stains that have an excitation wavelength around 470 nm. Examples of compatible stains are: SYBR Green, GelGreenTM, SYBR Safe, SYBR Gold, and SYPRO Ruby. The instrument's amber lid allows direct viewing of the fluorescent samples, with no need for separate glasses. The new blue light illuminators are available in two versions: the BLstar 9, a handy solution for mini gels, and the BLstar 16, for gels up to size of 16 cm x 20 cm.

MULTIPLEX ANALYSIS

The Image Lab 4.1 software is designed for use with the ChemiDoc MP imaging system. The new software automates multiplex analysis, providing a faster, more accurate alternative for quantifying protein expression levels via Western blotting. Proving that quantitative differences of a target protein are a result of biological differences and not experimental variability (such as unequal protein loading) is critical when conducting Western blotting experiments. Typically, scientists comparing gene expression levels use a housekeeping protein (HKP) as a loading control with the assumption that HKP expression does not change based on treatment type or sample origin. However, total protein normalization has been shown to be more stable than HKP normalization regardless of conditions or sample type and saves time by eliminating the need for multiple probing steps or optimizing a multiplex blot. With Image Lab 4.1 software, researchers can deliver normalized intensities automatically.

PRIMARY ANTIBODY DETECTION

The WestVision Peroxidase Polymer Anti-Rabbit IgG and Peroxidase Polymer Anti-Mouse IgG are designed to detect primary antibodies of mouse or rabbit origin in Western blots. The WestVision conjugates are based on a new method of polymerizing enzymes and attaching these polymers to antibodies that can allow a higher density of enzymes to access a target with lower interference. With a chromogenic substrate, the reagents can provide enough working solution to typically detect 20 to 200 blots (10 cm x 10 cm). With a chemiluminescent substrate, dependent on the dilution employed, 800 to 8,000 blots can be detected.

CHEMILUMINESCENT KITS

A new line of chemiluminescent Western blot kits provide the most sensitive detection solutions yet. Forty-five percent of researchers recently surveyed cited a lack of sensitivity and time to results in Western blots as significant challenges. The search for increased sensitivity can amplify background, requiring lengthy optimization to manage signal:noise contrast. SignaLOCK ChemiWestern kits solve these challenges. Each kit is composed of a chemiluminescent substrate, a non-protein blocker, and unique wash solution. All kits offer comprehensive protocols and can be used with both nitrocellulose and PVDF members. Whether using film or an imager, there is an optimized kit that will enable researchers to see more signal with less background in their Western blots.

LOW PROTEIN BINDING MICROTUBES

Low protein binding microtubes are now offered in both 0.5 mL and 1.5 mL volumes. These tubes are specially designed to meet the requirements of protein analytics while maximizing protein recovery rates. The microtubes are manufactured from a proprietary polypropylene with low protein binding properties and do not feature any potentially contaminating surface coating like silicone. The low protein binding microtubes feature an attached flat SafeSeal cap with a tooth-like cam that locks over the tube rim, increasing retaining strength and providing a reliable, secure closure. Tubes can be centrifuged to 20,000 x g, and are polymerase chain reaction (PCR) Performance Tested, and therefore certified to be free of human DNA, DNase, RNase, and PCR inhibitors.

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  • Newly offered instrumentation, apparatus, and laboratory materials of interest to researchers in all disciplines in academic, industrial, and governmental organizations are featured in this space. Emphasis is given to purpose, chief characteristics, and availabilty of products and materials. Endorsement by Science or AAAS of any products or materials mentioned is not implied. Additional information may be obtained from the manufacturer or supplier.

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