Science Webinar Series

Proteomics Meets Cellular Signaling: Exploring Post-translational Modifications by Mass Spectrometry

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Science  26 Apr 2013:
Vol. 340, Issue 6131, pp. 499
DOI: 10.1126/science.340.6131.499-c

Summary

Post-translational modifications (PTMs)—including phosphorylation, acetylation, and ubiquitination—are specific modifications that can alter the activity of an individual protein target. The cumulative effect of these small modifications is the regulation of large signaling pathways and networks within cells.

Mass spectrometry is a powerful tool for the identification of individual PTMs during the investigation of signaling networks. A new approach using antibody-based peptide enrichment for a class of PTMs combined with tandem mass spectrometry has been successfully used for global identification of hundreds to thousands of PTMs within a sample. In this webinar, examples of how the technology was applied to each of these PTM classes will be described.