New Products

Science  10 May 2013:
Vol. 340, Issue 6133, pp. 766
DOI: 10.1126/science.340.6133.766-a


The Accel-NGS DNA Library Kit is the first product in a new line of kits for next generation sequencing (NGS) sample preparation. NGS users can now produce polymerase chain reaction (PCR)-free libraries with as little as 5 ng of input DNA. The highly efficient Swift adaptation technology eliminates the need for PCR, thereby minimizing base composition bias and fidelity issues while reducing the input requirement. The unique, two-step adaptation process also reduces adapter dimer formation to maximize sequencing output. Unlike other kits, the Accel-NGS DNA Library Kit does not require intact double-stranded DNA, making it ideal for FFPE and damaged samples. The Accel-NGS DNA Library Kit protocol is fast, requiring only 75 minutes start-to-finish, and consists of five easy steps, two of which are bead-based separations that eliminate the need for time-consuming, electrophoretic gel-based size selection. In addition, the streamlined Accel-NGS workflow can be readily automated.


The NEBNext Microbiome DNA Enrichment Kit uses a novel method to separate microbial DNA from human host DNA, thereby reducing the prohibitively high cost of sequencing microbiome DNA to a practical level. Microbiome samples are commonly dominated by host DNA (up to 99%). This complicates genetic analyses of these samples, particularly total microbiome DNA sequencing. Since only a small percentage of sequencing reads pertain to the microbes of interest, obtaining sufficient sequence coverage of the microbiome DNA becomes cost-prohibitive or even technically infeasible. The NEBNext Microbiome DNA Enrichment Kit utilizes the MBD2-Fc protein, which binds to CpG-methylated DNA (including human genomic DNA) with very high specificity. The MDB2-Fc protein is attached to Protein A Magnetic Beads, enabling quick and easy removal of the contaminating host DNA in about 30 minutes. The microbial DNA-enriched sample is then ready to be processed for multiple downstream applications, including next generation sequencing, real-time polymerase chain reaction (qPCR), and endpoint PCR.


The NEXTflex 16S V4 Amplicon-Seq Kit has been developed to simplify bacterial metagenomics studies using Illumina HiSeq and MiSeq platforms. This kit allows users to go from sample to sequence in two hours, making it the fastest library prep kit available. Using specialized NEXTflex primers that target the V4 region of the 16S subunit, a single polymerase chain reaction amplification simultaneously ligates the necessary sequencing and barcoded region for multiplexing. The entire workflow requires only one clean-up step, maximizing recovery. Ideal for studies of microbiome community composition or comparative metagenomics, the NEXTflex V4 Amplicon-Seq Kit is available with up to 48 barcodes. Higher degrees of multiplexing are available on a custom basis.


A new comprehensive suite of SeqCap EZ Reagent kits are available for application in single or multiplex target enrichment experiments prior to DNA Sequencing. Designed to maximize customer convenience and streamline the DNA preparation workflow, the new kits provide customers with an all-inclusive reagent solution for use in their target enrichment experiments such as sequencing the whole exome or large target genomic regions. The new SeqCap EZ Reagent kits are also optimized and validated for use with the SeqCap EZ Library probe pools. The multiplexing capabilities of SeqCap EZ Library allow researchers to manage multiple samples per sequencing run enabling high throughput research laboratories to capitalize on cost-effective and efficient workflow methods for next generation sequencing. The SeqCap EZ Reagent Kits include the accessory, oligonucleotides, hybridization, and wash kits, all conveniently configured in a 24 reaction pack.


Two new additions to the extensive range of automated pipetting systems are uniquely tailored for polymerase chain reaction setup and nucleic acid purification, yet retain the flexibility that allows their use as open systems for diverse automated liquid handling applications. The new epMotion P5073 and M5073 workstations automate and simplify what are traditionally complex, labor-intensive pipetting tasks, saving time and improving the reliability and reproducibility of results. The epMotion M5073 fully automates the process of DNA purification, providing reproducibility with high yield and purity. Setup time is short and elution volumes as low as 25 μL for high concentrations can be used. Eppendorf's MagSep reagent kits are specifically designed for use with the epMotion M5073. These ready-to-use reagents, supplied in a specific tray, eliminate the need for manual buffer handling and require only room temperature storage.

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  • Newly offered instrumentation, apparatus, and laboratory materials of interest to researchers in all disciplines in academic, industrial, and governmental organizations are featured in this space. Emphasis is given to purpose, chief characteristics, and availabilty of products and materials. Endorsement by Science or AAAS of any products or materials mentioned is not implied. Additional information may be obtained from the manufacturer or supplier.

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