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Science  31 May 2013:
Vol. 340, Issue 6136, pp. 1122
DOI: 10.1126/science.340.6136.1122-a

SAMPLE PREP AND LC SYSTEM

The Prelude SPLC sample preparation and liquid chromatography system has been developed to make analysis of target compounds in complex biological matrices routine for clinical research and forensic toxicology laboratories. LC-MS/MS offers significant advantages in selectivity and quantitative accuracy over immunoassays and other analytical techniques. The Prelude SPLC sample preparation and liquid chromatography system is designed to work seamlessly with Thermo Scientific mass spectrometers. The Prelude SPLC system is designed to enhance the cost effectiveness of LC-MS/MS instrumentation by automating sample preparation and increasing mass spectrometer throughput. Automation can minimize variability inherent in manual sample preparation procedures while also reducing sample preparation time and effort. Innovative TurboFlow technology is designed to negate problems caused by sample matrix interference while capturing analytes of interest. This level of sample clean-up can enhance MS quantitative analysis by reducing ion suppression.

PROTEIN PROTEOLYSIS

Trypsin/Lys-C Mix, Mass Spectrometry Grade, is a unique combination of Trypsin Gold and rLys-C proteases. The new mix increases peptide generation with in-solution protein digestion and works with even difficult-to-digest proteins. Enhanced proteolytic activity achieved through the use of Trypsin/Lys-C Mix can result in a 20%–40% increase in the number of peptides generated, enabling a higher number of proteins to be identified. Trypsin/Lys-C Mix is also tolerant to Trypsin-inhibiting contaminants, enabling researchers to generate mass spectrometry data from poor-quality sample material such as FFPE sections. Many proteins are resistant to trypsin due to tight conformation, and although protein denaturation enables proteolysis, trypsin is inactive under these conditions. The Trypsin/Lys-C Mix overcomes this barrier as it remains active even under strong denaturing conditions, enabling digestion of even proteolytically resistant proteins.

MASS SPECTROMETRY INSTRUMENT

The Microsaic 4000 MiD represents the culmination of over a decade of research, bringing together two high-tech fields—chemical analysis and silicon microengineering—to make possible a transformative capability in detection. The mass spectrometry instrument is easily accommodated in a standard fume hood and, unlike any conventional system, does not require a cumbersome floor pump or separate PC. The 4000 MiD is readily integrated with existing chromatography equipment, and is ideally suited to reaction and process monitoring. Consuming only a fraction of the power demanded by conventional instruments, the Microsaic 4000 MiD is also in tune with modern green laboratory working practices. Its low operating cost and small footprint make the instrument deployable in a wide range of applications that mass spectrometry has not been able to reach—until now. The core technologies are chip-scale versions of traditional mass spec components which can be interchanged rapidly by the user.

HEAT-STABILIZATION SYSTEM

A heat-stabilization system has been demonstrated to be compatible with MALDI imaging analysis. The preservation of sample components prior to MALDI imaging analysis is crucial to accurately measure the distribution and abundance of biological molecules in organs. During downstream analysis, the presence of metabolites, drug compounds, and endogenous peptides, which exist at very low levels in the tissue sample, is often difficult to detect due to rapid degradation of the molecule of interest or masking by protein fragments generated from normal degradation processes. Utilization of Denator's Stabilizor system has been shown to stop degradation from the moment of sampling. This leads to increased accuracy and quality of analytical results. This development ensures that researchers who heat-stabilize tissues upstream achieve the best possible analytical results downstream.

MYCOBACTERIAL LIBRARY

The MALDI Biotyper (MBT) system allows for an instantaneous identification of colonies from a plate and covers a broad range of more than 4,600 microbial isolates from gram-negative bacteria, gram-positive bacteria, yeasts, multicellular fungi, and mycobacteria. Microbial identification with the MALDI Biotyper is done using a proteomic fingerprint. This unique species-specific pattern is automatically compared with reference spectra in the MALDI Biotyper library. In addition, the MALDI Biotyper supports the Open Microbiology Concept which allows customers to generate their own database entries from regional isolates via a push-button storage in a customer-specific sub-library. The new second edition of the MBT Mycobacteria Library adds another 140 isolates from 37 new species. The library is fully compatible with all standard cultivation media for mycobacteria, such as solid Löstein-Jensen medium or in liquid culture using the MGIT system from Becton Dickinson. With these added capabilities the MALDI Biotyper covers now more than 130 species of mycobacteria.

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  • Newly offered instrumentation, apparatus, and laboratory materials of interest to researchers in all disciplines in academic, industrial, and governmental organizations are featured in this space. Emphasis is given to purpose, chief characteristics, and availabilty of products and materials. Endorsement by Science or AAAS of any products or materials mentioned is not implied. Additional information may be obtained from the manufacturer or supplier.

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