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Science  02 Aug 2013:
Vol. 341, Issue 6145, pp. 574
DOI: 10.1126/science.341.6145.574-a

DNA SEQUENCING KIT

The NEXTflex Rapid DNA-Seq Kit enables construction of sequencing-ready libraries in less than two hours, with a flexible input range from 1 ng to 1 μg of DNA. A bead-based, gel-free size selection protocol and the availability of up to 96 unique adapter barcodes facilitate high-throughput applications. The NEXTflex Rapid DNA-Seq Kit is ideal for use with genomic DNA, FFPE samples, ChIP DNA, and low-input clinical samples. This kit also features Enhanced Adapter Ligation Technology, offering improved ligation efficiency and resulting in library preps with a larger number of unique sequencing reads.

RNA SEQUENCING KIT

The SMARTer Universal Low Input RNA Kit for sequencing combines the patented SMART (Switching Mechanism at 5' End of RNA Template) technology for double-stranded cDNA synthesis with random priming to facilitate transcriptome sequencing (RNA-Seq) from degraded total RNA samples acquired via common sample preparation techniques such as formalin-fixed, paraffin-embedded tissue and laser capture microscopy. The SMART protocol greatly reduces handling of the RNA sample, thereby minimizing the risk of sample loss and preserving the original message. The efficient incorporation of known sequences at both ends of the cDNA during first strand synthesis enables researchers to perform the entire protocol in a single tube, without a separate adaptor ligation step. The SMARTer Universal Low Input RNA Kit for Sequencing broadens the applications of RNA-Seq to include very low amounts of total RNA that are either compromised or are from sources that do not have polyadenylated mRNA.

qPCR ASSAY PLATES

The new PrimeTime qPCR Assay Plates have been designed for high throughput real-time polymerase chain reaction (qPCR) analysis. Primers and probes for 5'-nuclease and intercalating dye assays (e.g., SYBR) can now be directly ordered online in a 96-well plate format, eliminating the time-consuming transfer of primers and probes from reagent stocks, and streamlining the reaction setup. PrimeTime qPCR Assay Plates offer an ideal solution for many applications that generate large quantities of data, such as validation of NGS and microarray data as well as high throughput gene expression screening. Addressing the needs of each particular qPCR assay requirement, a range of primer and/or probe concentrations are available, delivered lyophilized within a 96-deep–well plate. For additional flexibility, different dye-quencher combinations can also be ordered on the same plate, extending the range of assays that can be performed during a single run.

CHROMATIN PREPARATION KIT

The PureEpi Chromatin Preparation and Optimization Kit provides a systematic approach to address the technical challenges of optimizing the preparation of chromatin by providing a complete set of validated reagents specifically designed for the generation of chromatin for ChIP experiments. The materials provided in this kit are ideal for both the optimization of chromatin preparations as well as the generation of chromatin in multiple sample types starting from either cultured cells or tissues. While other kits enable extraction of chromatin from cells, this is the first kit to provide a set of buffers, detailed methods, and optimization guidelines specifically designed for generation of sheared chromatin for ChIP. The protocol provided in the kit outlines a standard approach for chromatin preparation and includes tips to avoid common pitfalls.

DNA TARGET ENRICHMENT KIT

The SeqCap EZ Pure Capture Bead Kit supports target enrichment workflows prior to DNA sequencing. The newly introduced SeqCap EZ Pure Capture Bead Kit is designed to provide sensitive and robust methods for capture and purification of DNA sample libraries enabling high-quality targeted sequencing results. With the addition of the new kit to its capture portfolio, Roche now offers SeqCap EZ Library probe pools and all target enrichment reagents in a one-stop shop and a comprehensive set of reagents and probe pools for superior target enrichment performance. The probe pools include exome libraries, gene panels, and custom designs. A suite of SeqCap EZ reagent kits were released earlier this year for applications in single or multiplex target enrichment experiments. With the recent addition, the comprehensive set of capture reagents will allow a more streamlined target enrichment workflow and ensure superior sequence capture efficiency.

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  • Newly offered instrumentation, apparatus, and laboratory materials of interest to researchers in all disciplines in academic, industrial, and governmental organizations are featured in this space. Emphasis is given to purpose, chief characteristics, and availabilty of products and materials. Endorsement by Science or AAAS of any products or materials mentioned is not implied. Additional information may be obtained from the manufacturer or supplier.

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