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Science  24 Jan 2014:
Vol. 343, Issue 6169, pp. 441
DOI: 10.1126/science.343.6169.441-a

HYPOXIA CHAMBER

The new 856-HYPO Hypoxia Chamber from Plas-Labs is ideal for tissue culture work, including tumor cell research and stem cell research in hypoxic conditions. It features a color operated touchscreen display for automatically controlling and monitoring oxygen levels, temperature levels, and carbon dioxide levels. The 856-HYPO includes an easy-to-read 8" touchscreen monitor, inert gas hook-up, ethernet port connection for data logging, thermoelectric assembly, and password protection. The oxygen range is 0%–25% and the carbon dioxide range is ±0%–20%. The 856-HYPO Hypoxia Chamber also includes the standard two-year warranty for Plas-Labs' products.

HARDWARE AUTO FOCUS AND CLEANROOM KIT

A new Hardware Auto Focus and Cleanroom Kit for the Axio Imager Vario microscope system is now available. An integratable autofocus system ensures fast and precise focusing. The Hardware Auto Focus is ideal for surface inspections on reflective, low-contrast samples. In transmitted and reflected light, the focus system ensures high precision down to 0.3 of the depth of field of the objective lens. The sensor registers changes to the focus position and automatically and quickly compensates for any deviations via direct access to the z drive of the microscope. As a result, even large samples such as monitors and solar panels in xy direction remain constantly in the focus of the observer. Axio Imager Vario is certified for cleanroom use in accordance with DIN EN ISO 14644-1 and, with the cleanroom kit, fulfills the requirements of ISO 5.

BACTERIAL CULTURE GROWTH SYSTEMS

The EnPresso B range of reagent-based growth systems for bacterial cultures has been developed to address common challenges with bacterial growth such as insufficient yield, quality problems, and poor protein solubility or low activity. EnPresso B, EnPresso B Animal-free, and EnPresso B Defined Nitrogen-free can be used to significantly increase the yield of 15N-labeled proteins for NMR analysis. For ease of use, the products are supplied as pre-sterilized tablets, which are simply added to sterile, filtered water. Optimized protocols enable researchers to go from pre-culture to harvest within two days, using standard lab equipment such as shake flasks or 24 deep-well plates. The EnPresso B range incorporates EnBase technology which offers a novel approach to control the feeding, and thereby growth rate, of microbial cultures. A glucose-releasing agent breaks down long chains of polysaccharides, releasing glucose units to feed the microbes. As well as glucose, EnPresso tablets provide other key elements to support growth and control pH.

TISSUE SAMPLE FREEZING

Two easy-to-use devices designed for the fast freezing of tissue samples and specimens are now available. Ideal for enzyme studies, the Histology Bath features a -60°C (-76°F) working temperature that freezes tissue samples quickly, preventing microscopic ice crystals from developing and samples from distorting. With its flat, -34°C (-30°F) surface, the Histology Freeze Plate enables histotechnicians to observe tissue freezing directly and keep specimen edges flat when preparing samples for examination. The Histology Bath has a 1 L stainless steel reservoir and includes an immersion basket and chuck for the easy submersion and retrieval of samples. The bath work area measures 10.2 cm in diameter by 10.2 cm deep (4 x 4 inches). The unit is available for either 120V, 60Hz or 240V, 50Hz operation. The Histology Freeze Plate features a 877 cm2 (136 in2) working surface that accommodates multiple samples simultaneously and has been shown to reduce overall sample processing time by 40% or more.

BIOLUMINESCENT ASSAY

A non-horseradish peroxidase (HRP)-dependent, plate-based bioluminescent assay for detecting reactive oxygen species (ROS), ROS-Glo H2O2 Assay is designed to specifically detect hydrogen peroxide (H2O2) in both enzyme and cell-based applications. The ROS-Glo H2O2 Assay measures the activity of enzymes that generate or eliminate H2O2, and possesses many benefits over other commercially available assays for small molecule screening, including low false hit rate, signal stability, and compatibility with liquid handling. The assay can also be used to measure changes in the level of ROS by directly detecting H2O2 in cultured mammalian cells after two reagent additions, with no cell sample preparation required, reducing variability and the number of cells required. Due to the bioluminescent format and high sensitivity, ROS-Glo H2O2 Assay can be multiplexed with a variety of other cell-based assays to gain additional data from a sample.

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  • Newly offered instrumentation, apparatus, and laboratory materials of interest to researchers in all disciplines in academic, industrial, and governmental organizations are featured in this space. Emphasis is given to purpose, chief characteristics, and availabilty of products and materials. Endorsement by Science or AAAS of any products or materials mentioned is not implied. Additional information may be obtained from the manufacturer or supplier.

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