News this Week

Science  11 Apr 2014:
Vol. 344, Issue 6180, pp. 134
  1. Around the World

    1 - London
    Biomedical Hub 'MedCity' Launches
    2 - Brussels
    Pro-Life Groups Target E.U. Stem Cell Research
    3 - Sydney, Australia
    Scientists Alarmed Over Dwindling Antarctic Research
    4 - Washington, D.C.
    NASA to Staff: No Fraternizing With Russia


    Biomedical Hub ‘MedCity’ Launches

    New ground.

    The Crick institute will open in 2015.


    London's media-friendly mayor, Boris Johnson, unveiled an initiative this week to attract investment to the bioscience research powerhouses of London, Oxford, and Cambridge. Johnson hopes the plan—dubbed MedCity—will forge a "golden triangle," bringing in venture capital and luring pharmaceutical companies to set up shop.

    At the heart of the plan are two centers due to open in 2015: the $1 billion Francis Crick Institute, slated to be the biggest biomedical research facility in Europe, and an offshoot from Imperial College London that will host a $250 million multidisciplinary research center. Other London universities also have major expansion plans.

    Backed by $2 million from Johnson's own budget and $4.9 million from the Higher Education Funding Council for England, MedCity will coax researchers, companies, and investors to collaborate. "MedCity will span everything from research to clinical trials to manufacturing, across biotech, med tech, and health tech," said Johnson at the launch event. But MedCity may struggle to emulate its model—Boston—because of the distances between the cities. "Proximity is important," says Alan Barrell of Cambridge's Judge Business School, a MedCity adviser.


    Pro-Life Groups Target E.U. Stem Cell Research

    A coalition of European pro-life organizations has launched a fresh legislative attack against E.U. funding for embryonic stem cell research. The groups have organized a European citizens' initiative, a democratic novelty that gives citizens the right to propose legislation if they gather at least 1 million signatures from seven member states. The organizers of the initiative, backed by 1.7 million people in all 28 E.U. countries, were scheduled to meet with research commissioner Máire Geoghegan-Quinn and the European Parliament this week.

    Under a delicate compromise, E.U. funds can be used to study embryonic stem cells, but only in countries where such research is legal, and only using embryos left over from in vitro fertilization procedures. The new initiative proposes a ban on funding for any activities in which embryos are destroyed, not just in research but also in public health and development aid. Experts say it's hard to predict what the European Commission, which must consider turning the proposal into legislation, will do; it has until 28 May to respond.

    Sydney, Australia

    Scientists Alarmed Over Dwindling Antarctic Research

    Australia's peak scientific body, the Australian Academy of Science, warned last week that the country's territorial claims to Antarctica and its strategic position in the region are at risk due to a serious decline in scientific effort there. Since 1997, the number of Australian projects there has plummeted from 142 to just 62 this year, and from 1999 to 2006 the number of peer-reviewed publications on Antarctica by Australian researchers dropped from about 200 a year to less than 100. The drop in performance is a result of federal cost-cutting, which has hit both research and infra structure such as refueling, resupplying, and restaffing three bases. The warning that Antarctic research is "chronically under invested" comes in the academy's submission to the Australian government's 20 Year Australian Antarctic Strategic Plan.

    Of the seven countries to make territorial claims to Antarctica under the 1961 Antarctic Treaty, Australia's claim of 43% is the largest and is based on its geographic proximity and history of exploration, discovery, and activity in the frozen continent. But "national claims are not sovereign claims," says Will Howard, deputy chair of the academy's National Committee for Antarctic Research. "They imply responsibility for managing the claim and the offshore territory."

    Washington, D.C.

    NASA to Staff: No Fraternizing With Russia

    All together.

    Russian, Japanese, Belgian, and U.S. astronauts on the ISS in 2009.


    After an internal memo revealing the policy change leaked, NASA officials last week announced that they were suspending the agency's collaborations with Russia in light of its incursion into Ukraine's territory. However, the announcement appears to be purely symbolic: The one key existing area of partnership—using Russia's Soyuz capsules to ferry NASA astronauts to the International Space Station (ISS)—will be insulated from the new policy. "NASA and Roscosmos [the Russian space agency] will … continue to work together to maintain safe and continuous operation of the International Space Station," according to a statement issued by NASA on 2 April.

  2. Newsmakers

    Three Q's


    Saudi Prince Sultan Bin Salman Bin Abdul-aziz Al-Saud wants to change how the kingdom's subjects think about their pre-Islamic past. The 57-year-old science enthusiast, who 3 decades ago was the youngest—and the first Muslim and Arab—astronaut to fly on NASA's space shuttle, is now president of Saudi Arabia's Commission for Tourism and Antiquities. He oversees a $1.7 billion effort to build new museums and welcome foreign archaeologists; he also recently helped uncover an ancient hand ax (shown) at an excavation. This month, he is in the United States for the opening of the "Roads of Arabia" traveling exhibit.

    Q:Why are you so excited about Arabia's prehistoric past?

    S. A.-S.:I go to places and touch things. We're taking local police, governors, university staffs, and kids to excavate—then they can own it. It is quite a challenge for us, but archaeology is not separate from who we are. In the early years, people wanted to have nothing to do with historic sites. All walks of life, including religious scholars, are now visiting pre-Islamic sites.

    Q:You feature human and animal images in the exhibition. Isn't this a taboo among some Muslims?

    S. A.-S.:Artifacts that people thought were taboo are just objects made by other civilizations. When there was a recent find of ancient horse statues, King Abdullah went to look—we are very proud of our horses!

    Q:Are foreign and domestic women welcome at excavations?

    S. A.-S.:My deputy's wife is an archaeologist, and she certainly goes to sites. The women of Saudi Arabia have come a long way. And they have literally carried a lot of the history of Arabia on their backs. We just hired several of the smartest Saudi women to help with reinventing our museums.

    Bird Flu Scientist Allegedly Under Investigation

    Capua CREDIT: EU

    A story in Italian magazine l'Espresso published on 4 April claims that Italian prosecutors are investigating whether former avian influenza scientist Ilaria Capua, now a member of the Italian Parliament, trafficked in flu viruses and created a cartel to boost sales of a technology called DIVA, which she helped develop while head of the Istituto Zooprofilattico Sperimentale delle Venezie (IZSVe) in Legnaro.

    During her leadership at IZSVe, which ended with her election to the Chamber of Deputies in 2013, Capua became known for helping quash major bird flu outbreaks and starting a global campaign to allow scientists more access to genetic information from bird flu strains (Science, 10 November 2006, p. 918).

    The office of the Italian prosecutor could not confirm to Science that an investigation is taking place. Capua denies any wrongdoing and says the l'Espresso article is riddled with errors. "This is a pile of rubbish," says Capua, adding that she has filed a defamation suit against the magazine. She says she doesn't know whether the inquiry or the story might be politically motivated.

  3. Random Sample

    Experimental Lakes Lab Saved


    Canada's flagship environmental research center, the Experimental Lakes Area (ELA), has been under threat of closure for 2 years after its funding was pulled by the government. But ELA has found a savior: It will leave government hands and will be managed by the International Institute for Sustainable Development (IISD), a Winnipeg-based think tank. The 1 April announcement guarantees that the 46-year-old field site in northwestern Ontario will survive, at least for another 5 years.

    The ELA, which conducts experiments in a system of 58 lakes, has an impressive research record: Its scientists were the first to find evidence for acid rain, and to fully diagnose the effects of pollutants such as mercury, phosphate, and synthetic hormones on aquatic life.

    IISD President Scott Vaughan says that he intends to build upon this past research, while looking to expand the scope of the facility's science to investigate the effects of micropollutants and climate change on aquatic systems. The deal will hopefully bring the ELA some "stability," says freshwater ecologist Diane Orihel. But, she adds, IISD's plan to invite back former ELA scientists might prove difficult. "Some scientists got frustrated and took other positions."

    They Said It

    "Many scientists have been severely distressed by the outcomes of the L'Aquila trial and tend to share critical information only among themselves."

    —Italian geologist Gianluca Valensise, on changes in scientists' and the public's interaction 5 years after an earthquake struck L'Aquila, killing 309 people. The disaster led to a court case that found scientists guilty of manslaughter for misleadingly reassuring citizens after earlier tremors.

    Pterosaurs Take Manhattan!


    It's a bird! It's a plane! No, it's a pterosaur! These flying reptiles first appeared on Earth about 220 million years ago, just 10 million or so years after the earliest dinosaurs. They were excellent fliers, the first and the largest vertebrates to evolve that ability. (Birds and bats are the only other vertebrates that engage in true muscle-powered flight.) On 5 April, the largest ever pterosaur exhibit in the United States—"Pterosaurs: Flight in the Age of Dinosaurs"—opened at the American Museum of Natural History in New York City. Visitors have until 4 January 2015 to view the wonders on display.


    Among them: Life-size models of the largest pterosaur known, Quetzalcoatlus northropi (below, wingspan 10 meters), and the smallest, Nemicolopterus crypticus (above, wingspan 25 centimeters); eight real fossil specimens, including one of long-tailed, beak-snouted Ramphorhynchus with its wing membranes preserved; and "Fly Like a Pterosaur," an interactive exhibit that allows visitors to pilot bug-hunting pterosaurs over forests, seas, and volcanoes. Visitors can also try their hand at a pterosaur trading card game developed by Big Apple teenagers.

    The exhibit is brimming with fun facts, including what pterosaurs ate (fish, insects, and algae), how they walked on land (on all fours, with their wings tucked up like folded umbrellas), and their evolutionary relationship to other creatures (dinosaurs are their closest relatives). Pterosaurs ruled the skies for some 150 million years until they went extinct along with the dinosaurs about 66 million years ago, but they've come back to life in the Big Apple.

    By the Numbers

    $240 million—Amount of new money pledged by the Bill & Melinda Gates Foundation, the World Bank, and other donors to combat 10 neglected tropical diseases in Africa by 2020.

    23—Number of square meters of living space that the National Institutes of Health decided is enough for an individual chimpanzee—one-fourth the area that an advisory committee had recommended.

    85,000—Number of requests for high-skilled (H-1B) visas submitted by companies and universities within a few days of the start of the new application cycle—matching the maximum number of visas the United States can issue in the next fiscal year.

  4. Mesoamerica's Mystery Killer

    1. Jon Cohen

    Scientists have come up with a gallery of rogues to explain an epidemic of kidney disease in Central America. But the culprit has stayed one step ahead.

    Beat the heat.

    Cane cutters in El Paisnal, El Salvador, start work at the break of dawn to take advantage of the cooler morning hours.


    SAN SALVADOR AND USULUTÁN DEPARTMENTS, EL SALVADOR—A half-hour before sunrise, Emmanuel Jarquin pulls his four-wheel-drive pickup off the road near the rural town of El Paisnal and rumbles onto a sugar cane field. Jarquin, a physician, parks and steps into the sweet and smoky air, the cane having been charred the day before to burn off leaves and thorns. He walks toward the headlamps of several machete-wielding men who will start cutting at first light.

    "What are you bringing us?" one of the men cries in the darkness.

    "I'm bringing you health," Jarquin replies.

    Jarquin collaborates on a project studying a mysterious affliction of Central American agricultural workers known as chronic kidney disease of unknown etiology (CKDu). First reported in 2002, CKDu has no known links to diabetes or hypertension, the main causes of progressive renal damage worldwide. Much about CKDu is clouded by sketchy health statistics and the difficulty of distinguishing it from other kidney disease. But it appears to be spreading in the hot lowlands of Central America's Pacific Coast—and concerns are rising. In October, the Pan American Health Organization (PAHO) declared CKDu a "serious public health problem," with case reports streaming in from southern Mexico, Guatemala, Honduras, Nicaragua, Costa Rica, and Panama. In El Salvador alone, PAHO's latest figures say chronic kidney disease of all causes kills at least 2500 people in the country each year, and it's the main reason adults die in Salvadoran hospitals.

    The disease follows a peculiar pattern. It's about three times more common in men than in women. Cane cutters have been especially hard hit, but it's killing other agricultural workers as well. Whereas traditional CKD is mainly a disease of the elderly, CKDu disproportionately strikes young adults, especially those who toil long hours in the heat. But like its more common cousin, CKDu inexorably destroys the kidneys until they can no longer filter waste from the blood, forcing victims to receive dialysis or a kidney transplant to survive. For many poor agricultural workers in this region, such costly treatments are out of reach.

    As public health officials struggle to cope with what many are calling an epidemic, the origins and cause of CKDu remain a stubborn riddle. Possible culprits include dehydration and heat stress, pathogens, agrochemicals, heavy metals, a biochemical disorder, nonsteroidal anti-inflammatory drugs, antibiotics, low birth weight, and genetic susceptibility. Just as with a similar illness in the Balkans that baffled scientists for decades (see p. 146), hard data are scarce and strong opinions are abundant. It's also difficult to disentangle the science from politics. In Nicaragua, former sugar cane workers with the disease and their families have staged demonstrations demanding compensation, igniting violent showdowns with police that included a fatal shooting of a protester in January.

    But with increasing attention from researchers, community advocates, the sugar cane industry, and officials in affected countries and abroad, the research landscape is shifting markedly, with a slew of new studies under way or in the works. "The international global health community needs to take this very seriously," says Peter Hotez, a pediatrician at Baylor College of Medicine in Houston, Texas, who specializes in neglected tropical diseases and edits the Public Library of Science journal by that name. "What we're seeing is not business as usual. This is a serious outbreak and we need all hands on deck."

    Early detection.

    Ramón García Trabanino, who now runs a dialysis center in San Salvador, reported a rise of a baffling chronic kidney disease in 2002.


    A hot lead

    Osmin Sorto, 49, lives in Tierra Blanca de Jiquilisco, an agricultural community near the coast in a region of El Salvador called Bajo Lempa, and says he has watched more than 100 people die of CKD. He could easily have been one of them.

    At 15, Sorto started harvesting cotton and planting flags for crop-dusters, but at 22 he landed a plum government job, complete with health care. His kidney problem came to light in 1998, after a doctor's visit for an inflamed toe. Tests revealed gout due to high uric acid levels, and his doctors started closely monitoring his kidney function. He did not have diabetes or hypertension, but by 2005, he had developed end-stage renal disease. Sorto, a classic case of CKDu, was offered a kidney from his sister, and he had the rare insurance plan in El Salvador that would cover the cost of the transplant. Still, he initially was reluctant to accept his sister's kidney. "I didn't want to expose her to any risks," Sorto says. Finally, faced with an imminent death, he acceded, and both are doing fine today.

    Few CKDu patients are so lucky. Hospital Nacional Rosales, in El Salvador's capital, San Salvador, is a palatial complex built of Belgian iron in the 1890s. The 525-bed landmark—El Salvador's largest public hospital—is always full. When nephrologist Zulma Cruz started working there 13 years ago, her ward had 18 beds for peritoneal dialysis, which drains kidney toxins from the abdomen, and 16 hemodialysis machines that do the more sophisticated cleansing of toxins from the blood. Today, the hospital has 85 beds filled with patients on peritoneal dialysis and 90 more coming each day to take turns on 30 hemodialysis machines. Each month, up to 70 new patients with end-stage renal disease show up seeking dialysis; the hospital can offer the life-extending treatment to only a few dozen each month. The others must seek out private care, or, more commonly, return home to die.

    A young doctor in training at the hospital, Ramón García Trabanino, first brought CKDu to light. "The whole hospital was flooded by renal patients," remembers García Trabanino, who began working at the hospital in the late 1990s. "I thought, 'Why are all these people here with kidney disease? It's not normal.' " An adviser suggested he do a study.

    Over 5 months, García Trabanino interviewed 202 new patients with end-stage renal disease. Medical records and personal histories uncovered an obvious cause for CKD in only one-third of the patients, equally split between men and women. Of the rest, 87% were men and the majority worked in agriculture and lived in coastal areas, he and his co-authors reported in September 2002. Their report in the Pan American Journal of Public Health speculated that patients who had CKD with características peculiares might have developed the disease after exposure to herbicides and insecticides.

    Health officials took little interest in this greenhorn's findings. "I spoke with PAHO and I remember them laughing at me," García Trabanino says. "They thought I was crazy." The Ministry of Health in El Salvador took no action, but it did give him an award for his study. "The judges must have been drunk that night," he says.

    García Trabanino, who now runs a private dialysis clinic, understands why this landmark report received little traction. "It was very weak," he says. "If I could travel back in time, I'd erase half of what I wrote and do more testing. But that's what we could do back then."

    A newspaper article about the award caught the attention of Julio Miranda, a leader of a social fund for health emergencies in Tierra Blanca de Jiquilisco. Miranda's team contacted García Trabanino and explained that they had seen an extraordinary rise in deaths from kidney failure since the mid-1990s. "Many people thought it was pesticides," Miranda says. They agreed to collaborate with García Trabanino on a study that would compare men in the Jiquilisco lowlands with men in a region 500 meters above sea level. García Trabanino thought that workers at higher altitudes might be exposed to different occupational and environmental factors.


    Reports of chronic kidney disease of unknown etiology have stretched along the Pacific coast from southern Mexico down to Panama.


    The results, published in Nefrología in 2005, showed a puzzling pattern. In both the lowlands and the higher region, most men worked in agriculture, and pesticide use was equally high in both areas. But the researchers found an elevated CKD incidence only on the coast. "I had to accept my hypothesis was wrong," García Trabanino says. He and his collaborators were dumbfounded. "I asked, 'What's different in the coastland?' "

    He could only think of one thing: the heat.

    Researchers who had documented CKDu in other countries shared his suspicions. In November 2005, the Program on Health and Work in Central America, or SALTRA, gathered García Trabanino and 17 other researchers from six countries for a workshop in León, Nicaragua. The meeting sparked studies in Nicaragua and El Salvador that would survey nearly 2000 people living at different altitudes and working in various occupations.

    The studies measured two indicators of kidney damage: creatinine levels and glomerular filtration rate, or GFR. (As the kidney's filtration system breaks down, creatinine levels rise.) Both studies found elevated creatinine levels and suppressed GFRs only in the lowlands. SALTRA founder Catharina Wesseling, an epidemiologist at the Central American Institute for Studies on Toxic Substances at the National University in Heredia, Costa Rica, worked on both studies and says they deliver a clear message. "We think the evidence of heat stress and chronic dehydration is very strong," says Wesseling, who refers to the disease as Mesoamerican nephropathy. She and her collaborators hypothesize that strenuous work in hot climates repeatedly depletes bodily fluids, overtaxing kidney cells. She notes, however, that "it's still very unclear how the disease starts and who will be affected."

    One population does seem to be especially vulnerable, Wesseling says. "Those who are dying are sugar cane workers and they are really dying every day and they are dying younger and younger." Why the disease has yet to be found in parts of Latin America with similar climates and large sugar cane industries, such as Cuba, the Dominican Republic, and Brazil, is baffling, but Wesseling says it may have to do with better working conditions or medical care—or a lack of awareness. "My guess is the disease is more common than we know."

    Wesseling is collaborating with a team led by nephrologist Richard Johnson of the University of Colorado, Denver, that has a provocative idea for CKDu's cause: Repeated dehydration disrupts a biochemical pathway, leading to excess conversion of glucose to fructose. The enzyme fructokinase, which metabolizes the fructose, then creates high levels of kidney-damaging uric acid, oxidants, and immune system messengers that cause inflammation.

    Johnson and co-workers tested their hypothesis by subjecting mice to hours of high heat at a stretch while restricting water intake in one group but not in another. They repeated the experiment in mice that could not metabolize fructose because their fructokinase gene had been intentionally crippled. As they reported online in December in Kidney International, kidney damage occurred only in normal mice on restricted water, providing compelling support for what some now call the "heat-plus" hypothesis.

    Johnson wonders whether people who develop the disease might become dehydrated and then, to quench their thirst, drink sodas that are high in fructose, compounding the problem. "Rehydrating with soft drinks," he suggests, "may toast the kidneys."

    García Trabanino says the fructokinase hypothesis is "the closest thing to reality we have right now." But he still suspects that other factors have yet to be identified. So he is launching a new study with Jarquin, the four-wheel-drive doctor, to examine more carefully the differences between people who live in the coast and at higher altitudes. García Trabanino chose to work with Jarquin because he is one of the few doctors who specialize in treating agricultural workers: In addition to conducting occupational health evaluations and training for agricultural companies, he runs a private practice in his boyhood town of El Paisnal, which means he has unusually close ties to the sugar cane–cutting community. The two physicians have unusually close ties to each other, too: García Trabanino's father, also a nephrologist, treated Jarquin's father, himself a victim of traditional chronic kidney disease.

    A dissenting voice

    Next to a cornfield in Bajo Lempa's Jiquilisco municipality, the epicenter of CKDu in El Salvador, is a small unmarked cemetery with 2 dozen aboveground tombs. Most are recent, adorned with wreaths and fresh flowers. CKD took the lives of one woman and nine men in this graveyard, says local doctor Lilian Núñez. One was her uncle. "This man was diagnosed 3 days before he died," she says.

    At her side in the cemetery is Carlos Orantes, a nephrologist at the National Institute of Health in San Salvador—and a skeptic of the heat-plus hypothesis. "We are not obsessed with pesticides, but we do believe they're at the center of the cause of the disease," Orantes says. His team's 2009 CKD study in Bajo Lempa confirmed many aspects of the disease's demographics. But it left Orantes convinced that pesticides deserve a closer look.

    Orantes thinks the coastal regions may have more CKDu because the heat increases susceptibility to nephrotoxic effects of the pesticides, the true cause. He also contends that pesticides may interact with heavy metals such as arsenic to poison the kidneys. He points to research from Sri Lanka suggesting that glyphosate in pesticides chelates arsenic, boosting its level in the water and its potential to damage the kidneys' delicate filtering machinery. And his team has preliminary evidence that arsenic levels are higher in fields surrounding the cemetery than in the nearby village.

    Wesseling, who has built her career around studying the impact of toxic substances, says his arguments have no merit. She says neither pesticides nor arsenic can explain the geography of the disease. Arsenic, she says, is prevalent in soil and lakes across El Salvador, not just where CKDu is common. "It doesn't convince me at all," she says. "He's only looked at one specific place."

    Cruz of Hospital Nacional Rosales agrees. "We need other hypotheses," she says. To that end, she and her co-workers teamed up with researchers from the Karolinska Institute in Stockholm to study kidney biopsies from CKDu patients. In a November report published in the American Journal of Kidney Diseases, they described how the disease inflicted consistent, distinct damage on the kidneys in the eight subjects, a finding that many researchers hope may provide a tangible new lead.

    Political maelstrom

    The scientific uncertainty has not deterred finger-pointing. Many agricultural workers with CKDu are convinced that pesticides are the cause and are demanding compensation from government funds set aside for health emergencies. Sandra Peraza, a chemist at the University of El Salvador in San Salvador and part of SALTRA, attended a community meeting in Bajo Lempa a few years ago where she was supposed to present data. "They were screaming about pesticides," she recalls. "I thought if I say right now that pesticides are not the answer, they'll kill me. The meeting finished without my having a chance to speak and I said, 'Thank God.' "

    The biggest CKDu study to date has only fed the rancor. In the center of this maelstrom is epidemiologist Daniel Brooks, who became aware of CKDu a decade ago because his wife worked on a sister-city project connecting Brookline, Massachusetts, and Quezalguaque, Nicaragua. "She said they have this problem of this chronic kidney disease and maybe they need an epidemiologist," says Brooks, who works at Boston University and had long studied smoking cessation and sunburn protection.

    At first, Brooks tried to help from afar. Then in 2008, a community group in Nicaragua filed a complaint with the World Bank Group's International Finance Corporation (IFC) that ultimately would catapult him to the front of the CKDu research field—and make him a lightning rod for criticism. IFC had approved a $55 million loan to Nicaragua Sugar Estates Limited (NSEL), in part so it could start producing ethanol. The complaint alleged that the company's environmental and health practices did not meet IFC loan terms. One allegation deserved special attention, an IFC ombudsman concluded: that working conditions were linked to widespread CKD.

    Bitter feud.

    Former cane cutters in Managua in March 2013 protested against sugar cane companies they believe are responsible for CKDu.


    As part of a mediation process, the company and a community group representing afflicted families hired Brooks's team—one of nine applicants—to investigate CKDu's cause. The group received $800,000 from the sugar company and another $250,000 from the IFC ombudsman's office for its studies. The researchers assessed everything from industrial hygiene to water quality, pesticide exposure, and biomarkers of kidney damage, comparing people who worked in sugar cane fields with those in factories or other hot settings like underground mines, construction, and shipping ports.

    The 2012 report from "the Boston group" had a decidedly equivocal bottom line. "Following an evaluation of all available data, our overall conclusion was that the causes of CKD in the Western Zone of Nicaragua were unknown and that the relationship between the disease and [the company's] work practices was also unknown," Brooks and his colleagues wrote.

    Wesseling and others were taken aback. "They went wrong," she says. "There's a clear link." She also objects to how the sugar cane industry in Nicaragua has used the Boston group's study to rebut criticism that it works people too many hours without enough shade, water, and rest. (El Salvador's sugar cane industry is dominated by worker-run collectives, which insulates it from such criticism.)

    Jason Glaser of the nonprofit La Isla Foundation, which does public health policy and human rights work related to CKDu, contends that IFC and NSEL influenced the results. "Both have a clear interest in the disease not being related to sugar cane work," Glaser says. "To say there is no relationship at this point is willful ignorance." (In February, the Dutch Postcode Lottery awarded La Isla $4 million, more than half of which Glaser says it will invest in CKDu research.)

    Brooks, who estimates the disease has prematurely killed 20,000 people, strongly rejects the assertion that industry funding influenced his group's findings. "We have the right to publish what we find, and the industry doesn't get to make a determination whether we publish it," he says. In fact, Brooks says he finds the heat-plus hypothesis the most compelling explanation of CKDu. "The nightmare from a scientific standpoint," he says, "is that there are a bunch of different things going on. It's hard to disentangle."

    But he's giving it another go. In collaboration with the U.S. Centers for Disease Control and Prevention (CDC), Brooks and his team are launching three new studies. One will assess how working conditions vary by occupation and geography in Nicaragua and El Salvador. A second will probe for genetic susceptibilities. Finally, his team will investigate whether kidney damage begins to occur before people enter the workforce. The nonprofit CDC Foundation will fund the project with industry money: $1.05 million from the Sugar Producers of the Central American Isthmus and $675,000 from Nicaragua's National Committee of Sugar Producers.

    Conducting research in a highly charged social and political atmosphere "makes the work a lot more challenging," says Reina Turcios-Ruiz, an epidemiologist based at CDC's Central American Regional Office in Guatemala City. "But it's an opportunity for us at CDC to demonstrate our integrity for the scientific process and to make available the highest levels of evidence," she says, "so decisions on scientific policy are based on evidence and not on myth or rumors."

    Flush with cash to launch more sophisticated studies, researchers may have their best chance yet to understand the baffling disease. On top of the satisfaction of solving a complex scientific mystery and removing the "u" from CKDu, their work raises hopes for interventions that can prevent the damage. And that, in turn, may help Emmanuel Jarquin live up to his promise of "delivering health" each time he visits the sugar cane fields.

  5. A Balkan Riddle's Serendipitous Solution

    1. Jon Cohen

    Starting in the 1950s, researchers hunted for the cause of an unusual chronic kidney disease afflicting farming communities in the Balkans. It took a mishap with Chinese herbal medicine in Belgium and the dogged sleuthing of molecular epidemiologists to arrive at the culprit.

    For researchers puzzling over a mysterious kidney disease in Central America dubbed CKDu (see p. 143), a tale from the Balkans offers inspiration and some cautions. In the late 1950s, reports started coming out of southeastern Europe about a kidney disease afflicting rural communities near tributaries of the Danube River. Balkan endemic nephropathy, a 1966 article in The Lancet declared, was “clearly a big public-health problem” whose cause “is utterly obscure and has excited much speculation.” Solving the mystery took half a century—and led down multiple blind alleys.

    Like CKDu, the Balkan disease had a geographic peculiarity: It was seen only in mountain valleys and lower elevations. Suspected causes were legion, including heavy metal poisoning, infectious disease, genetic predisposition, and allergies. The most intense attention fell on a common food contaminant, a mycotoxin called ochratoxin A, which often grows in stored grains and is known to damage kidneys.

    Proven guilty.

    Aristolochia clematitis has been fingered as a cause of chronic kidney disease.


    In 1969, a report in a Serbian journal added another suspect: seeds from a weed that grew in fields next to cultivated wheat commonly used to bake homemade bread. And in the early 1990s, a spate of kidney failures in Belgium provided a break in the case. A number of women taking Chinese herbs in a “slimming regimen” began to suffer a kidney pathology that mirrored that seen in Balkan endemic nephropathy. Like victims of the Balkan disease, the women also had a greatly elevated risk for a urinary tract cancer. “Chinese-herb nephropathy” soon turned up in several countries and was traced to ingestion of a cousin of the weed suspected in the Balkans. Both plants contain aristolochic acid, a known nephrotoxin and carcinogen.

    In 2005, pharmacologist Arthur Grollman of Stony Brook University in New York—who has long urged the U.S. Congress to regulate herbal medicine—sent a graduate student to Croatia to investigate. The student confirmed that the weed containing aristolochic acid still grew in wheat fields there. Grollman discovered that Croatian veterinarians in the 1950s extensively studied kidney failure in horses that had eaten the weed. His team also found molecular clues: Autopsied kidneys from victims of Chinese herbal and Balkan endemic nephropathy contained DNA–aristolochic acid complexes, and a mutation in tumors from the Balkan patients matched one seen in animal experiments with aristolochic acid.

    In the face of dogged skepticism, particularly from the ochratoxin A crowd, Grollman's team developed what many see as an ironclad case that aristolochic acid causes Balkan endemic nephropathy. The coup de grêce came with a cover report in the March 2012 issue of Kidney International, in which a leading nephrologist wrote that “aristolochic acid nephropathy” should become the name for both the Balkan and Chinese herb maladies.

    The long saga of the Balkan disease offers several lessons for CKDu researchers, Grollman says. Search for answers broadly at first, he says, and use the sophisticated tools of molecular biology to sift possibilities. And expect academic rivalries to slow progress. “The psychology is the same everywhere,” Grollman says. “If you're in Croatia and they put you in the national academy for all the work you've done on ochratoxin A, you're not happy when someone comes along and says that has nothing to do it.”

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