PerspectiveRNA Events

Cas9 Targeting and the CRISPR Revolution

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Science  16 May 2014:
Vol. 344, Issue 6185, pp. 707-708
DOI: 10.1126/science.1252964

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The ability to add, remove, or change DNA sequences is essential to studies that investigate the genetic underpinning of phenotypic traits. With its unprecedented efficiency and stunning ease of use, DNA editing technology based on the prokaryotic CRISPR (clustered regularly interspersed short palindromic repeats)–Cas9 system is completely revolutionizing genome engineering. In little more than a year, CRISPR-Cas9 editing has been implemented in a multitude of model organisms and cell types (1) and has already started to supplant incumbent genome editing technologies, such as TALENs (transcription activator-like effector nucleases) and ZFNs (zinc finger nucleases).