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Science  16 May 2014:
Vol. 344, Issue 6185, pp. 765
DOI: 10.1126/science.344.6185.765-a

ALGAE PROTEIN EXPRESSION KITS

The new algae engineering kits contain features requested by algae biologists and are designed to make work with algae simpler and faster. The GeneArt kits represent the first commercially available genetic modification and expression systems for photosynthetic microalgae Chlamydomonas reinhardtii and Synechococcus elongates. The GeneArt Chlamydomonas Protein Expression Kits and GeneArt Synechococcus Protein Expression Kits contain high-expression vectors capable of driving production of genes of interest to as much as 10% of total cellular protein. The kits also contain new proprietary transformation reagents that can boost introduction of DNA into cells by as much as a thousandfold over what is typically achieved with current techniques. The new GeneArt kits also contain reagents and a protocol enabling researchers to freeze and store Chlamydomonas strains at -80°C. Algal researchers have traditionally relied on passaging strains indefinitely, which makes the cultures subject to genetic drift, and shipping live cultures on agar slants, which frequently become contaminated.

3-D CELL CULTURE PLATES

The new Alvetex Scaffold 3-D cell culture plates provide biopharmaceutical companies with a new tool to improve the predictive accuracy, productivity, and biological relevance of their cell-based high throughput testing. The new 384-well plates allow scientists to grow tumor cells in a way that better mimics how these cells grow in vivo. It is then very simple to test the cytotoxic effects of novel or existing anti-cancer compounds using standard cell health assays such as MTT, XTT, extracellular oxygen consumption, and many others. Researchers can then easily retrieve RNA and protein from the drug-treated cells to study mechanism of action and look for biomarkers. Alvetex Scaffold plates work seamlessly with liquid handling robotic systems further enhancing the high throughput potential of your cell-based testing. Alvetex Scaffold is also available in other formats including a 12-well culture plate, 24-well culture plate, and 96-well plate as well as 6-well and 12-well inserts.

KIM-1 ELISA KIT

The most sensitive KIM-1 (human) enzyme-linked immunosorbent assay (ELISA) kit is designed for detection of acute kidney injury, diabetes, and toxicity. This quantitative ELISA kit offers ultrasensitive detection enabling quantification of both normal and disease state levels of KIM-1, detecting as little as 1.2 pg/mL of KIM-1 in human urine. The assay provides quantitative results with excellent reproducibility between experiments, and offers efficiency over Western blotting by requiring less sample input, and analysis of up to 38 duplicate samples in less than two hours. The ELISA displays high specificity to human KIM-1 with low cross-reactivity to similar molecules such as TIM-3 and TIM-4. The KIM-1 ELISA kit is complete, flexible, and put through rigorous fit-for-purpose validation and stability testing to ensure high precision, accuracy, sensitivity, and specificity. It is supplied with an easy-to-follow protocol, precoated microtiter plate, and liquid color-coded reagents to save the user time and reduce errors.

SEMIAUTOMATIC MICROPLATE SEALER

The MiniSeal II semiautomatic microplate sealer comes complete with adapters enabling it to process deep-well, shallow-well, low-profile deep-well, filter, and assay plates that conform to the ANSI/SLAS standard format. MiniSeal II plate adaptors not currently in use are ingeniously designed to stack on top of the unit to keep them readily available. The MiniSeal II heat sealer operates with a very high force, that together with a powerful 450W heater ensures high-integrity sealing of almost any type of microplate. With so much available power, sealing times for most polypropylene plates with Porvair seals are less than three seconds each. Sealed plates are automatically ejected from the MiniSeal II. The compact footprint, about the size of a sheet of A4 paper, means that MiniSeal II will fit just about anywhere—on your lab bench or inside a fume hood if required.

NUCLEIC ACID DYE SYSTEM

The QuantiFluor ONE dsDNA System is a sensitive, dsDNA-specific fluorescent dye suitable for use in both research and clinical workflows. The new dye system is integrated into the new Promega Quantus Fluorometer and GloMax detection instruments, but is compatible with any fluorometer capable of measuring the appropriate fluorescence excitation and emission spectra. The QuantiFluor ONE dsDNA System is supplied in "add-and-read" format, enabling users to simply quantitate even low-concentration samples with confidence, and without dilutions or additional tubes. The dye system has significantly increased sensitivity compared with absorbance-based systems and is highly specific to dsDNA, showing minimal binding to ssDNA, RNA, protein, and interfering compounds. The QuantiFluor ONE dsDNA System expands the company�s range of nucleic acid quantitation reagents and instrumentation, which includes the recently launched Quantus Fluorometer for highly sensitive (up to 40,000x higher than absorbance-based methods) quantitation of dsDNA, ssDNA, and RNA.

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  • Newly offered instrumentation, apparatus, and laboratory materials of interest to researchers in all disciplines in academic, industrial, and governmental organizations are featured in this space. Emphasis is given to purpose, chief characteristics, and availabilty of products and materials. Endorsement by Science or AAAS of any products or materials mentioned is not implied. Additional information may be obtained from the manufacturer or supplier.

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