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Science  18 Jul 2014:
Vol. 345, Issue 6194, pp. 343
DOI: 10.1126/science.345.6194.343-a

RNA FFPE Kit

The Maxwell 16 LEV RNA FFPE Kit for RNA isolation from mammalian formalin-fixed, paraffin-embedded (FFPE) tissue samples. The new kit is designed to rapidly extract pure, high yield RNA from thin sections of FFPE tissue samples, with consistency and time saving provided by the automated Maxwell 16 instrument. The purified RNA is suitable for downstream applications including reverse transcription polymerase chain reaction (RT-PCR), RT-qPCR, endpoint RT-PCR, cDNA library synthesis, and gene expression analysis. Specifically designed for optimal purification of RNA from 5 μm sections of FFPE samples, the new kit maximizes RNA yield and eliminates the need to concentrate the extract prior to amplification, minimizing sample waste and the need for reruns. The kit is very simple to use, with the automated walkaway system able to extract from 16 samples in a single run. Lysates are placed directly into the cartridges, and RNA is ready for amplification in approximately 60 minutes (after proteinase K digest).

Transfection Reagent

The FectoPRO is an optimized transfection reagent which has beendesigned for high yields in medium- and large-scale production of proteins and antibodies in mammalian cells. FectoPRO delivers very high protein yields in CHO and HEK cell line systems. FectoPRO will also produce up tothree times more protein using half the DNA required by other leading commercial transfection reagents. In addition, scientists working with transient gene expression systems using FectoPRO will be able to benefit from reproducibility experiment-to-experiment and between reagent batches. The FectoPRO transfection reagent offers scientists working in pharmaceuticals and biotechnology specific advantages over other commercial transfection reagent offerings for bio-production. The reagent is well characterized in terms of chemical structure and is supplied ready-to-use. FectoPRO is also manufactured and formulated using a highly specific and controlled production process in Polyplus' dedicated manufacturing facility.

Oligonucleotide Delivery Agent

A novel vitamin modifier has shown in initial tests to have the potential of improving cell delivery of oligonucleotides. The new reagent, 5'-Niacin-CE Phosphoramidite, is easily incorporated during solid phase oligo synthesis. The niacin-based modifier offers several advantages over the use of conventional lipophilic delivery agents, including reduced risk of in vivo toxicity, and removes the necessity of cleaving the delivery reagent once in the cell. These benefits make vitamins an attractive method for the delivery of therapeutic oligonucleotides. While lipophilic modifier reagents have been shown to enhance cell penetration, vitamin-mediated cell delivery offers a distinct advantage due to the fact that vitamins are required, but not produced by cells. As such, it is believed that interaction with a specific binding protein is required before the vitamin-oligo conjugate is internalized. Not only does this enhance delivery and overcome the risk of toxicity, but it also offers some exciting potential for cell targeting.

Target-Enrichment Kit

The SureSelectQXTReagent Kit is a revolutionary next generation sequencing target enrichment solution that produces sample-to-sequencing-ready-libraries in just seven hours with only 50 ng of input gDNA. Designed to meet the exacting needs of clinical researchers for a fast, easy, same-day sample-to-sequencing-workflow, SureSelectQXTkits are three times faster than existing transposase-based methods and require 30% less hands-on time. They are also optimized for use with samples of limited availability and provide superior coverage of genomic targets for confident variant calling. Combined with the fastest sequencers on the market, these kits make sample-to-data in 24–36 hours a reality.SureSelectQXTkits' unprecedented speed is achieved by coupling transposase-based library preparation with major advancements in hybridization chemistry, significantly decreasing hybridization time from 16 hours to 90 minutes while maintaining high performance. Its proven hybridization technology enables the sensitivity and specificity required for the complete and accurate variant analysis of exomes or custom genomic regions.

Tumor Panel

Fully validated on formalin-fixed, paraffin-embedded samples, the new 60-gene next generation sequencing hybridization-based SureSeq Solid Tumor Panel offers researchers accurate and reliable solid tumor profiling for both known and novel variants. The content of the panel has been defined by recognized cancer experts, covering key genes for a range of cancer types. All exons of these genes are fully covered, including mutation hotspots, enabling both detection and discovery of known and novel variants, respectively. The hybridization-based SureSeq Solid Tumour Panel minimizes polymerase chain reaction bias and duplications commonly associated with alternative enrichment methodologies, enabling greater run-to-run consistency. This is particularly important in situations where there is limited sample or where the ability to detect minor allele frequencies is required. Such sample types require a highly uniform and sensitive enrichment and OGT's expert bait design ensures this by providing efficient and improved uniformity of coverage of the targeted regions.

  • Oxford Gene Technology

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  • www.ogt.com

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  • Newly offered instrumentation, apparatus, and laboratory materials of interest to researchers in all disciplines in academic, industrial, and governmental organizations are featured in this space. Emphasis is given to purpose, chief characteristics, and availabilty of products and materials. Endorsement by Science or AAAS of any products or materials mentioned is not implied. Additional information may be obtained from the manufacturer or supplier.

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