Science Webinar Series

Techniques for RNA detection: Where are we headed?

Science  22 Aug 2014:
Vol. 345, Issue 6199, pp. 954
DOI: 10.1126/science.345.6199.954-c


As the messenger of genetic information, RNA plays an important regulatory role in cell and tissue development as well as during disease progression. Understanding the best ways to work with RNA and the various RNA detection methods can help scientists advance our understanding of gene expression patterns and elucidate the roles of different genomic elements in cellular function and dysfunction. A variety of RNA detection methods–including Northern blotting, in situ hybridization, and reverse transcription polymerase chain reaction–are already available; however, each comes with its own advantages and limitations in signal sensitivity, specificity, and stability. Additionally, most often detection is carried out on lysed cell samples, so a significant amount of information is lost. This webinar will bring together two experts who will share their knowledge and expertise in a variety of RNA detection methodologies, including one that utilizes live cells.