Science Webinar Series

Expanding the scope of RNA-Seq: From stem cells to FFPE

Science  05 Sep 2014:
Vol. 345, Issue 6201, pp. 1195
DOI: 10.1126/science.345.6201.1195-c


As next generation sequencing (NGS) platforms advance in their speed, ease-of-use, and cost-effectiveness, many researchers are transitioning from microarrays to RNA sequencing (or RNA-seq) for their gene expression analysis needs. RNA-seq goes beyond differential gene expression to provide fundamental insights into how genomes are organized and regulated. Some RNA-seq platforms also offer higher sensitivity, increased sample number flexibility, and the ability to analyze highly degraded or rare samples from as little as 10 ng of input RNA. During this webinar, our expert speakers will discuss how NGS and RNA-seq can be broadly applied, including for the analysis of gene regulation through allelic expression and long non-coding RNAs, particularly in pharmacogenomics; for identification of novel microRNAs and transcript isoforms in stem cells; or for the discovery of new tumor biomarkers in archived formalin-fixed, paraffin-embedded samples.