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Science  03 Oct 2014:
Vol. 346, Issue 6205, pp. 109
DOI: 10.1126/science.346.6205.109-a

NGS Library Preparation Kit

The epMotion automation method is now available for the TruSeq Stranded mRNA library preparation kit for next generation sequencing as "Illumina Qualified" (Illumina Qualified indicates that libraries prepared with the epMotion method have been shown to be comparable to those prepared manually). For next generation sequencing systems a variety of sample preparation kits are available. These kits are needed to convert either DNA or RNA samples into sequencing ready libraries, a procedure that includes many steps and can be time consuming. RNA sequencing requires additional steps—either the depletion of unwanted ribosomal RNA or the positive selection of mRNA from total RNA samples. The epMotion method can be used for the automated construction of 8, 16, or 24 libraries starting with 100–1,000 ng of total RNA together with Illumina's TruSeq Stranded mRNA kit. The overall hands-on time is less than 1.5 hours, while the total run time of the entire procedure is ~11.5 hours for 24 samples.

Genomic Editing Tool

CRISPR/Cas9 is revolutionizing the field of genomic editing by providing scientists with a powerful tool able to change any gene, in any cell in a highly targeted manner and without introducing foreign DNA. This latest tool in genome editing allows for specific genome disruption and replacement in a flexible and simple system resulting in high specificity and low cell toxicity. The CRISPR/Cas9 genome editing system requires the co-expression of a Cas9 protein with a guide RNA vector expressed from the human U6 polymerase III promoter. With the protospacer-adjacent motif (PAM—the sequence NGG) present at the 3' end, Cas9 will unwind the DNA duplex and cleave both strands upon recognition of a target sequence by the guide RNA. The functional cassette synthesized in the rescue donor vector can then be inserted into the unwound DNA. The repaired genome will now express your desired sequence with or without tags.

Gene-Knockout Kits

New gene-knockout kits provide a complete solution for researchers to knock out a human gene at the chromosomal level. This genome wide offering provides an unprecedented convenience for genome editing using the CRISPR technology. OriGene has always been focused on providing state-of-the-art reagents of highest quality. Its comprehensive offering of expression cDNA clones, shRNA/siRNA, recombinant proteins, and antibodies are widely utilized in the biomedical research community worldwide and are cited in thousands of publications. A kit is provided against every human gene locus, containing two gRNAs and a compatible donor vector with a functional cassette. In addition to gene knockout, this kit can also be used for promoter strength study for each gene locus. The predesigned nature of the kit greatly reduces the researcher's time and effort using this platform.

ChIP Sequencing Kit

Combining chromatin immunoprecipitation with massively parallel sequencing (ChIP-seq) allows the accurate survey of interactions between proteins and DNA, enabling the study of epigenetic marks which is essential for a full understanding of transcriptional regulation of genes. Chromatrap is a new quicker, easier, and more efficient way of performing ChIP-seq assays. It uses discs of an inert, porous polymer to which Protein A or Protein G has been covalently attached to maximize the capture efficiency of the target chromatin/antibody complex. Chromatrap utilizes the solid-state technology in parallel with high throughput sequencing to deliver a precise ChIP-seq protocol from small cell numbers and chromatin concentrations. Specifically adapted for greater chromatin concentrations, Chromatrap ChIP-seq now combines the dynamic range of Chromatrap with the downstream analysis power of deep sequencing, allowing the genome wide identification of transcription factor binding sites and specific DNA associated protein modifications with no limitation in scale and resolution. 

DNA-Sequencing Kit

The ThruPLEX DNA-sequencing (DNA-seq) Kit has high multiplexing capability with improved performance for Illumina Next Generation Sequening (NGS) Platforms. ThruPLEX DNA-seq builds on the innovative ThruPLEX chemistry to generate DNA libraries with expanded multiplexing capability and even greater diversity with lower duplication rates. The ThruPLEX chemistry is the only single-tube, three-step library prep kit for low input (0.05 ng to 50 ng) amounts, which is especially useful for clinical samples such plasma, other biofluids, and FFPE samples. Kits contain up to 96 Illumina-compatible indexes pre-dispensed and sealed in linear barcoded microplates. ThruPLEX DNA-seq can be used in any DNA-seq, RNA-seq, or ChIP-seq application and offers robust target enrichment performance with all of the leading platforms.

DNA Barcodes

The NEXTflex Dual-Indexed DNA Barcodes are designed for multiplexing of up to 192 samples for library preparation. These barcodes are compatible with high throughput automation for increased scale and cost efficiency. The NEXTflex Dual-Indexed Barcodes can be used to multiplex genomic DNA, RNA, and ChIP sequencing libraries. The NEXTflex Dual-Indexed DNA Barcodes allow pooling of multiple library preparations in a single flow cell lane. The indexes are designed to be sequencing error resistant and allow for consistent and proper differentiation between samples.

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