Science Webinar Series

Innovations in light sheet microscopy: Strategies and new applications

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Science  24 Oct 2014:
Vol. 346, Issue 6208, pp. 489
DOI: 10.1126/science.346.6208.489-c

Summary

Over the past 10 years, light sheet microscopy (or selective plane illumination microscopy, SPIM) has transformed the microscopy field, offering a faster, less phototoxic technique than conventional methods that can create true 3-D images. Ideal for observing living organisms and the cellular dynamics of biological systems, this method uses a unique illumination approach to achieve high penetration depths, fast imaging speeds, and subcellular-level resolution. Because a specimen is illuminated with a sheet of light rather than a focused laser beam, only regions directly exposed to light will fluoresce—creating minimal photo-induced tissue damage. In this webinar, the speakers will discuss their own uses of light sheet microscopy and provide insight into its different applications, including open source SPIM and the dynamic behavior of subcellular components within live specimens.