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Science  12 Dec 2014:
Vol. 346, Issue 6215, pp. 1384
DOI: 10.1126/science.346.6215.1384-a

Western Blot Imaging System

Until now, digital imaging systems have failed to deliver the sensitivity and resolution of film. The new ChemiDoc Touch System, however, allows detection of faint bands missed by film and produces publication-quality images. The ChemiDoc Touch outperforms film in other ways as well. When using film to image abundant proteins, strong bands quickly saturate and become unquantifiable. Saturated bands can also obscure the signal from adjacent faint bands, making Western blotting with film challenging. The ChemiDoc Touch addresses these issues through its wide dynamic range, which permits easy and reliable quantification even of highly abundant proteins, and through the instrument's ability to optimize exposure for each protein of interest. Capturing images with the ChemiDoc Touch is easy. The ChemiDoc Touch offers a smooth, intuitive user experience that makes capturing, reviewing, selecting, and exporting images efficient and straightforward. The imager also allows stain-free imaging, a technology exclusive to Bio-Rad.

Quantitative Western Blot System

The Amersham WB system is a fully integrated system for quantitative SDS-PAGE and Western blotting of proteins using fluorescence detection. The system is designed to drive down the variability seen with conventional Western blotting. With the Amersham WB system, every stage of the Western blotting process, including electrophoresis, transfer, probing, and scanning, is standardized and monitored—resulting in consistent, quantitative protein analysis. Western blotting requires a complex combination of analytical knowledge, extensive manual handling, dexterity, and strict adherence to protocols in order to achieve reliable results. Failure rates, however, can be as high as 25% and data variations between different users can be as high as 35%–40%. To address this shortfall in consistency, GE Healthcare Life Sciences has developed the Amersham WB system, with a standardized traditional Western blotting workflow that typically achieves a variability of less than 10% between users. The system's consistency and reproducibility delivers robust quantitative data.

Protein A Kit

The Gyrolab Protein A Kit is designed for use on the Gyrolab xP workstation. During purification of biotherapeutics, Protein A can leach from the chromatography support and co-elute with the therapeutic antibody product. The contamination complicates subsequent analysis using immunoassays. In addition, Protein A can elicit an immune response. It is therefore essential to accurately quantify Protein A during the purification process, and prior to product release. The new Protein A kit, designed and validated for use with Gyros nanoliter-scale immunoassay platform, includes automated acid dissociation of Protein A and IgG during sample preparation, to ensure contamination is accurately asasessed. Gyros has incorporated proprietary antibodies to ensure high specificity and sensitivity. The easy-to-use kit quantifies native Protein A, recombinant Protein A variants, and MabSelect SuRe ligand, and has fewer manual steps than other standard enzyme-linked immunosorbent assay kits, reducing hands-on time and risk for error.

Phosphorylation Detection Assays

AlphaLISA SureFire Ultra assays are designed to reduce assay time and to improve the protein phosphorylation detection process. With high sensitivity and wide dynamic range, AlphaLISA SureFire Ultra assays are quick and easy to run with a minimal amount of sample, significantly reducing time and expense. The AlphaLISA SureFire Ultra assays use TGR BioSciences' CaptSure technology to eliminate antibody interference in the therapeutic antibody screening process. AlphaLISA SureFire Ultra sensitivity and dynamic range also make it ideal for other types of screening, such as small molecule modulation, detection of low and basal levels of phosphorylation, and detection of phosphorylation in tissue lysates. The assays also leverage PerkinElmer's AlphaLISA technology that has better spectral characteristics related to hemoglobin interference, which can otherwise hinder the accuracy of measurements. AlphaLISA SureFire Ultra generates results in less than two and a half hours with only one or two incubation steps and no washing.

Remote Access Imaging System

The vibrant new T:Genius imaging system allows high-performance, walkaway imaging of gels and blots on a smartphone or tablet. Using the T:Genius' "StatusLink" feature, researchers can stay updated and even share image results with colleagues, no matter whether they are down the hall or in another country. This is a great time saver when, for instance, scientists are developing a long-exposure chemi blot and want to see how it's doing while having lunch or busying themselves with something else in the lab. The new T:Genius is both stylish and practical, comes in a choice of exciting colors, and features a sensitive camera that will give high-res images. With the powerful GeneSys image capture software, researchers can set up their imaging protocols, click, walk away, and see accurate DNA or protein results later in the lab or on their tablet or phone.

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  • Newly offered instrumentation, apparatus, and laboratory materials of interest to researchers in all disciplines in academic, industrial, and governmental organizations are featured in this space. Emphasis is given to purpose, chief characteristics, and availabilty of products and materials. Endorsement by Science or AAAS of any products or materials mentioned is not implied. Additional information may be obtained from the manufacturer or supplier.

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