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Antimicrobial peptide resistance mediates resilience of prominent gut commensals during inflammation

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Science  09 Jan 2015:
Vol. 347, Issue 6218, pp. 170-175
DOI: 10.1126/science.1260580

Gut microbes resist inflammation

It is vital to human well-being that our gut microbiota can be distinguished from harmful, but often very similar, organisms. Cullen et al. begin to analyze how one dominant symbiont, Bacteroidetes thetaiotaomicron, does this. Our guts release potent antimicrobial peptides when we become infected with pathogenic bacteria such as salmonella, but these symbionts make an outer lipopolysaccharide coat that differs from those of pathogens by only one phosphate molecule. Enzymatic removal of this group is enough to confer resistance to the host's immune response and allow the symbionts to escape damage.

Science, this issue p. 170

Abstract

Resilience to host inflammation and other perturbations is a fundamental property of gut microbial communities, yet the underlying mechanisms are not well understood. We have found that human gut microbes from all dominant phyla are resistant to high levels of inflammation-associated antimicrobial peptides (AMPs) and have identified a mechanism for lipopolysaccharide (LPS) modification in the phylum Bacteroidetes that increases AMP resistance by four orders of magnitude. Bacteroides thetaiotaomicron mutants that fail to remove a single phosphate group from their LPS were displaced from the microbiota during inflammation triggered by pathogen infection. These findings establish a mechanism that determines the stability of prominent members of a healthy microbiota during perturbation.

Human gut microbial communities reside in an open ecosystem subject to disruptions ranging from dietary change to toxin exposure and pathogen invasion. Many of these perturbations, and functional disorders such as irritable bowel syndrome, are accompanied by nonspecific immune responses that disrupt community structure and function (1, 2). Host inflammatory mechanisms to remove harmful organisms and restrict bacteria to the lumen commonly target conserved molecular patterns found on pathogens and commensals alike, yet healthy gut microbial communities can remain stable for years in humans (3).

Because cationic antimicrobial peptides (AMPs) represent one of the most prominent and nonspecific components of the host response to pathogens, we determined the minimum inhibitory concentration (MIC) of polymyxin B (PMB) for a common human gut commensal, Bacteroides thetaiotaomicron. PMB is a bacterial cationic AMP with a mode of action similar to that of mammalian AMPs (4). Notably, this commensal exhibits 680- to 2400-fold increased resistance to PMB compared with mammalian enteropathogens or Escherichia coli (Fig. 1A, fig. S1, and table S1). A survey of 17 prominent human commensals, representing the three major bacterial phyla in the gut (5), revealed that resistance against multiple inflammation-associated human and murine AMPs is a general feature of the human gut microbiota (Fig. 1B, fig. S1, and table S1).

Fig. 1 Human gut commensals are highly resistant to cationic AMPs.

(A) MIC of PMB against E. coli and B. thetaiotaomicron. (B) MICs of human and mouse inflammation-induced AMPs and bacterial surrogates against prominent human gut commensal bacteria (blue) and enteropathogens (red). MICs of PMB and colistin were determined with E-test strips; others were determined with the microtiter broth dilution method. See also fig. S1 and table S1.

We screened transposon mutant populations of five human gut commensal species for genes required for fitness in the presence of PMB. This approach identified a single gene (encoded by BT1854 in B. thetaiotaomicron) that mediated PMB resistance in all species tested (Fig. 2A and tables S2 to S4). Targeted deletion and complementation of BT1854 confirmed this phenotype for multiple AMPs (Fig. 2B and table S1).

Fig. 2 Lipid A dephosphorylation mediates AMP resistance in prominent human gut bacteria.

(A) Heat map of the relative fitness of transposon mutant strains in the presence and absence of PMB. Columns indicate human gut bacterial species tested (abbreviations from Fig. 1B); rows indicate gene orthologs with insertions displaying a significantly altered fitness (q < 0.05) in the presence of PMB in at least one species. n/s, not significantly altered; n/a, no ortholog. (B) BT1854 (LpxF) is necessary for PMB resistance in B. thetaiotaomicron. Representative MIC assessed with the E-test method is shown. (C) LpxF is required for dephosphorylation of lipid A. Fourier transform–ion cyclotron resonance MS analysis of lipid A isolated from wild-type (red) and lpxF deletion mutant (blue) B. thetaiotaomicron revealed a shift of the predominant peak by ~40 m/z, consistent with the gain of a single phosphate group. Inset shows predicted structures and m/z values of the doubly deprotonated ([M-2H]2–) ions for monophosphorylated (red) and bis-phosphorylated (blue) penta-acylated lipid A. Minor peaks are consistent with tetra-acylated lipid A structures (fig. S2, C and D). (D) Deletion of lpxF increases cationic cytochrome c binding to B. thetaiotaomicron, indicating altered surface charge. (E) Increased PMB-Oregon Green (PMB*) binding to lpxF deletion mutant cells measured by fluorescence quantification (left panel) and microscopy (right panels). Error bars represent SDs and asterisks indicate significance (P < 0.01). Representative fluorescence microscopy images of bacterial cells incubated with PMB* (green) are shown as an overlay with 4′, 6′ diamino-2-phenylindole (DAPI) (blue). Scale bar indicates 2 μm. (F) LpxF protects the outer membrane from PMB perturbation. 1-N-phenylnaphthylamine (NPN) uptake profiles of select strains were measured followed by challenge with the indicated concentration of PMB. Arrowheads indicate the addition of NPN (20 s) and PMB (80 s). Readings were taken in 5-s intervals. Each experiment was performed in triplicate with representative results shown. See fig. S3 for complementation.

BT1854 has limited homology to the phospholipid biosynthetic enzyme PgpB, a phosphatidylglycerol phosphatase (6) that is not implicated in AMP resistance. However, an unusual PgpB homolog, LpxF, catalyzes the removal of the negatively charged 4′-phosphate group from the lipopolysaccharide (LPS) lipid A anchor in a small, phylogenetically heterogeneous group of AMP-resistant pathogens (79). Notably, B. thetaiotaomicron produces an underphosphorylated lipid A structure when compared to most Gram-negative organisms (10) (fig. S2, A and B).

Mass spectrometry (MS) analysis of lipid A extracted from B. thetaiotaomicron revealed a predominant peak at a mass-to-charge ratio (m/z) of 843.6, consistent with the published (10) doubly deprotonated ([M-2H]2–) ion structure of penta-acylated 4′-dephosphorylated lipid A (predicted exact mass: 844.7m/z) (Fig. 2C). By contrast, MS analysis of lipid A extracted from the B. thetaiotaomicron BT1854 deletion mutant revealed a peak consistent with the (M-2H)2– ion of penta-acylated, bis-phosphorylated lipid A (predicted exact mass: 884.6 m/z) (Fig. 2C). An additional minor peak consistent with a tetra-acylated structure is observed for all strains (Fig. 2C and fig. S2, C and D). Complementation restores the wild-type lipid A profile (fig. S2E). Based on these results, we designated this protein as LpxF.

Characterization of wild-type and lpxF mutant strains showed that LpxF increased resistance to inflammation-associated AMPs by neutralizing the negative charge of the cell (Fig. 2D), decreasing AMP binding at the bacterial surface (Fig. 2E and fig. S3A), and reducing AMP-dependent membrane disruption (Fig. 2F and fig. S3B).

If resistance to inflammation-associated AMPs determines Bacteroidetes fitness in the gut, then a strain with reduced AMP resistance should be outcompeted by an isogenic wild-type strain in a mammalian host during inflammation. We established a gnotobiotic model of gut inflammation by colonizing germ-free mice with a mixture of wild-type, lpxF deletion mutant, and complemented (lpxF,lpxF+) B. thetaiotaomicron strains 7 days before infection with Citrobacter rodentium, a murine enteropathogen that mimics human gastrointestinal infection (11), or an avirulent tir mutant incapable of inducing colitis (fig. S4, A to C) (12).

We next monitored the relative abundance of each strain over time in these animals. The B. thetaiotaomicron lpxF deletion mutant was rapidly displaced by wild-type and lpxF,lpxF+ complemented strains in mice infected with C. rodentium (Fig. 3A and table S5). Displacement occurred at the onset of inflammation (fig. S5, A and B) and concurrent with increased AMP secretion measured from colonic tissue explants (fig. S5C). By contrast, the lpxF deletion mutant was able to persist in mice that had not been infected with C. rodentium (Fig. 3B) and in mice infected with the noninflammatory C. rodentium tir mutant, which colonizes the gut to levels similar to those of the wild-type pathogen (Fig. 3C). The lpxF mutant was also outcompeted in gnotobiotic mice exposed to dextran sulfate sodium (DSS), a chemical inducer of intestinal inflammation, at the onset of inflammation (Fig. 3D; fig. S4, A to C; and fig. S5). Exposure in vitro to numerous AMPs, but not C. rodentium or DSS, recapitulates the in vivo results (fig. S4, D and E). LpxF thus appears to be a general determinant of resilience during inflammation and not a specific requirement for response to C. rodentium.

Fig. 3 AMP resistance determines the resilience of a prominent human gut symbiont in gnotobiotic mice with colitis.

Germ-free mice (n = 5 per group) were colonized with wild-type, the lpxF deletion mutant (solid lines), and complemented (lpxF,lpxF+; dashed lines) B. thetaiotaomicron strains 7 days before initiation of inflammation by C. rodentium infection (A); no further treatment (B), infection with a noninflammatory C. rodentium tir mutant (C), or exposure to 3% DSS ad libitum for 7 days (black bar) (D). Infection with C. rodentium strains is indicated by black arrowheads, and relative C. rodentium abundances are reported as percentage of total fecal DNA (shaded bars); error bars represent SD and asterisks indicate significant (P < 0.01) differences.

If resistance to AMPs is also important for resilience in the context of a multiphylum human gut microbiota residing in the mammalian gut environment, then a mutant sensitive to AMPs should exhibit a loss of resilience in this community. We colonized germ-free mice with 14 bacterial species, including wild-type B. thetaiotaomicron, that represent the three dominant phyla observed in the human gut (tables S6 to S8) (13). Notably, this community remained largely stable through the course of C. rodentium infection (Fig. 4A) and associated inflammatory events. To determine whether LpxF is required for this stability during perturbation, we replaced B. thetaiotaomicron with the lpxF deletion mutant. Unlike wild-type B. thetaiotaomicron, the lpxF mutant was rapidly and specifically displaced from the defined human gut microbiome upon C. rodentium infection and consequent host inflammation (Fig. 4B and fig. S6A). By contrast, in the absence of C. rodentium infection, both the B. thetaiotaomicron wild-type and lpxF mutant-containing communities remained stable for the duration of the experiment (fig. S6B).

Fig. 4 AMP resistance determines commensal resilience in the context of a human or murine gut microbiota.

(A and B) Germ-free mice (n = 5 per group) were colonized with 14 prominent human gut microbes, including B. thetaiotaomicron wild-type or lpxF deletion mutant, 7 days before infection with C. rodentium; community composition was monitored by species-specific quantitative polymerase chain reaction (QPCR) and reported as median percentage of total. Uninfected controls are shown in fig. S6B. (C and D) Specific pathogen-free Rag−/− mice (n = 5 per group) were gavaged (black arrowhead) with either B. thetaiotaomicron wild-type or lpxF deletion mutant; in (D), mice were infected with C. rodentium 7 days later (red arrowhead). Colonization levels were assessed by QPCR from fecal DNA. C. rodentium levels are reported as the percentage of total fecal DNA. Error bars indicate SD and asterisks indicate significance (P < 0.05). (E) AMP resistance is a general feature of human gut Bacteroidetes, Firmicutes, and Actinobacteria. Fecal samples from 12 unrelated, healthy human donors were cultured on varying PMB concentrations (x axis), and the number of species-level phylotypes (operational taxonomic units; OTUs) belonging to each phylum observed from each donor (points, colored by phylum) was normalized to the number observed in culture in the absence of PMB. A weighted (abundance) analysis provides similar results (fig. S8B).

Germ-free mice have an immature innate immune system (14) that is largely rescued by colonization with mouse, but not human, microbiota (15). We tested whether B. thetaiotaomicron requires LpxF in animals with a complete mouse microbiota and a mature innate immune system. Because human gut Bacteroides spp. are rapidly outcompeted in specific pathogen-free (SPF) wild-type mice (16), we screened multiple strains of mice and found that B. thetaiotaomicron, like B. fragilis (16), stably colonized SPF Rag−/− animals (Fig. 4C). In these mice, the B. thetaiotaomicron lpxF deletion mutant colonized to levels equivalent to those of the wild type (Fig. 4C). B. thetaiotaomicron resilience during C. rodentium infection of these SPF mice remained dependent on LpxF, indicating that the inflammation caused by pathogen infection, rather than other members of the commensal mouse microbiota, mediated the requirement for AMP resistance (Fig. 4D). Many innate immune defects observed in germ-free mice are also abrogated by segmented filamentous bacteria (SFB), a mouse commensal that interacts directly with the gut epithelium (15). The lpxF deletion mutant remained stable over time in gnotobiotic mice monoassociated with SFB, indicating that even a pure population of immunostimulatory commensal members of the host’s native microbiota does not induce a requirement for AMP resistance (fig. S6C).

LpxF orthologs are readily identified in all sequenced human-associated Bacteroidetes, and all characterized LPS structures in this phylum reveal an underphosphorylated lipid A structure (fig. S7), suggesting that all human gut Bacteroidetes use this mechanism to resist inflammatory perturbations. We conducted a human study to determine whether our observations from sequenced type strains extend to gut commensals captured directly from humans. To this end, we cultured 733 species-level phylotypes from 12 unrelated, healthy donors (table S9). These cultured strains represent ~98% of the donors’ original, uncultured communities at the phylum level, ~95% at the class and order levels, ~80% at the family level, and ~50% at the genus level (fig. S8A). Quantification of PMB resistance across these culture collections established that the AMP resistance profiles of fecal microbial communities isolated directly from humans mirror the phylum-level patterns observed in sequenced type strains (Fig. 4E and fig. S8B).

Understanding mechanisms of microbiota stability is important for efforts to manipulate these communities for therapeutic purposes. We found that the ability of prominent human gut commensal bacteria to resist killing by AMPs also mediated stability during infection. This resilience hinges on a protein that removes a single phosphate group from the bacterial LPS to determine whether the host maintains or removes commensal bacteria in response to inflammation. In this way, commensal-encoded mechanisms for persistence in the host during inflammation complement host-encoded mechanisms for immune tolerance of the microbiota (17). As observed in certain pathogens, lipid A modification may provide additional benefits to commensal microbes beyond AMP resistance, including reduced activation of the host Toll-like receptor 4-myeloid differentiation factor 2 (TLR4-MD2) complex that recognizes common forms of bacterial LPS (7, 8, 10, 1823). Our studies indicate that LpxF is dispensable in the absence of inflammation, suggesting that the broad conservation of this enzyme across commensal Bacteroidetes reflects selective pressures imposed by periodic inflammatory events. A delicate balance between microbial resilience and host tolerance thus allows for commensal persistence throughout a diverse range of perturbations while preventing commensal overgrowth or depletion, either of which could have deleterious effects on the host.

Supplementary Materials

www.sciencemag.org/content/347/6218/170/suppl/DC1

Materials and Methods

Figs. S1 to S8

Tables S1 to S9

References (2453)

References and Notes

  1. Acknowledgments: We thank E. Groisman, J. Galan, and the Goodman lab for helpful suggestions; C. Jacobs-Wagner for microscopy assistance; J. Leong for C. rodentium strains; and I. Ivanov for SFB. This work was supported by NIH grants DK089121, GM103574, GM105456, the Global Probiotics Council, and the Crohn’s and Colitis Foundation of America to A.L.G. NIH grants AI064184 and AI76322 to M.S.T.; and Army Research Office grant W911NF-12-1-0390 to M.S.T. All human studies were conducted with approval from the Yale University Human Investigation Committee, and the sequences reported in this paper have been deposited with the European Bioinformatics Institute under accession number PRJEB7697.
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