Science Webinar Series

Overcoming challenges in cellular analysis: Multiparameter analysis of rare cells

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Science  23 Jan 2015:
Vol. 347, Issue 6220, pp. 443
DOI: 10.1126/science.347.6220.443-c

Summary

There are a variety of challenges when studying rare cell types, primarily due to their very low abundance against a high background of other cells. Identifying ways to study these cell types and better understand their roles in different human diseases is important to advance basic biomedical research—especially elucidating and characterizing disease mechanisms and targets—as well as in medical diagnostics and therapeutics. With multiparameter capabilities and a very high analysis rate, flow cytometry is one of the technologies often utilized to analyze rare cells. During this webinar, the application of this technology to the isolation and analysis of rare cells will be discussed, with a focus on innate lymphoid cells (ILCs), circulating antigen-specific lymphocytes, and innate-like cells (such as natural killer T cells and circulating endothelial cells).