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Science  13 Mar 2015:
Vol. 347, Issue 6227, pp. 1274
DOI: 10.1126/science.347.6227.1274-a

Fluorescence Microscopy

The ZOE Fluorescent Cell Imager is one of the first cell imaging systems able to deliver the power of microscopy in a system that is as easy to use as a tablet. The system includes three fluorescent channels and brightfield to simplify fluorescence imaging for cell culture applications. Fluorescence microscopes are used in traditional cell culture workflows to confirm transfection efficiency and visualize fluorescent proteins. Performing this task requires training and the process can be time-consuming. Fluorescence microscopes can take 15 minutes to warm up and the systems are often located in a separate facility that is outfitted with a darkroom. However, the ZOE Fluorescent Cell Imager's LEDs are instantly ready to use after the power is turned on. Researchers simply place their culture dish onto ZOE's stage and then access all controls using the LCD touchscreen, tapping the screen to capture images and pinching with their fingers to zoom up to 20x magnification.

Human Class II MHC Monomer Reagents

ProM2 is a range of Human Class II MHC monomer reagents for tetramer analyses in the study of CD4+ T cell immune responses. ProM2 Class II MHC Monomers are pre-biotinylated and can be readily made into MHC tetramers that allow the detection of single antigen-specific CD4+ T cells accurately by flow cytometry. Class II tetramers made from ProM2 monomers can also be used to separate cells for culture, expansion, and further study. ProM2 Class II MHC monomer reagents are supported by ProImmune's expert customer service and application support, helping new users in particular to establish and optimize robust protocols in their own laboratories. The class II reagent offering complements ProImmune's existing extensive range of services for evaluating CD4+ T cell responses, including ProImmune REVEAL MHC peptide binding assays, CD4+ T cell and DC T cell proliferation assays, and standard immune monitoring services through ELISPOT and flow cytometry based assays.

Mitochondria/Metabolism Assays

The new range of 96- and 384-well phosphorescence- and fluorescence-based assays enable direct real-time analysis of mitochondrial respiration (MitoXpress Xtra), glycolysis (pH-Xtra), and intracellular oxygen concentration (MitoXpress Intra)—providing the perfect toolkit for monitoring bioenergentics, metabolism, Warburg, and mitochondrial function & toxicity. MitoXpress Xtra—an oxygen consumption assay (high-sensitivity method)—is a convenient fluorescence-based, high throughput approach to the direct real-time analysis of mitochondrial oxygen consumption rate. The easy-to-use assays measure oxygen consumption of isolated mitochondria, cell populations, small organisms, tissues, and enzymes. pH-Xtra Glycolysis Assay is a fluorescence-based assay that measures extracellular acidification. The assay detects changes in extracellular pH caused by the acid extrusion of either suspension or adherent cells. This allows convenient plate based analysis of glycolytic flux and can be used to monitor alterations in glucose metabolism and to confirm mitochondrial dysfunction.

NFκB Translocation Assay

The Amnis NFκB Translocation kit allows researchers to better study the nuclear translocation of NFκB, a transcription factor that plays a central role in regulating key mammalian cell processes, including proliferation, inflammation, immune, and stress responses. The new kit uses imaging flow cytometry to obtain statistically significant quantitative assessment of NFκB translocation as well as visual identification of the translocation at a single-cell level. The optimized kit, which works with cultured cell lines and whole blood cells, conveniently contains directly-conjugated anti-Human NFκB monoclonal antibody, 7-AAD dye, and required buffers. Using the dedicated Nuclear Localization Wizard in the IDEAS software, NFκB translocation can then be studied and quantified in an objective, statistically robust manner. The kit is designed for use with the Amnis ImageStreamX Mark II and the Amnis FlowSight imaging flow cytometers, systems that combine the quantitative power of flow cytometry with the spatial information provided by microscopy.

Cryopreservation Solution

PRIME-XV FreezIS DMSO-Free is a chemically defined cryopreservation solution for human mesenchymal stem/stromal cells (MSCs) free of dimethyl sulfoxide (DMSO) and animal-derived components. Amid growing concerns that DMSO may compromise the potency of MSCs, offering an effective DMSO-free cryoprotectant is an important addition to PRIME-XV product line. Researchers can now characterize cells in DMSO-free environments during basic and translational research, which may add valuable insight downstream and facilitate scale-up. PRIME-XV FreezIS DMSO-Free maintains the potency of MSCs throughout cryopreservation while sustaining comparable cell viability as solutions containing 10% DMSO. For applications where the effects of DMSO are not as critical, PRIME-XV FreezIS is offered as a 10% DMSO-containing, protein-free, chemically defined cryopreservation solution for a variety of cell types. Both cryopreservation solutions join the PRIME-XV line of cell therapy products, which are designed to offer the highest quality and performance for the culture of human stem cells and primary cells.

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  • Newly offered instrumentation, apparatus, and laboratory materials of interest to researchers in all disciplines in academic, industrial, and governmental organizations are featured in this space. Emphasis is given to purpose, chief characteristics, and availabilty of products and materials. Endorsement by Science or AAAS of any products or materials mentioned is not implied. Additional information may be obtained from the manufacturer or supplier.

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