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Response to Comment on “Principles of ER cotranslational translocation revealed by proximity-specific ribosome profiling”

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Science  12 Jun 2015:
Vol. 348, Issue 6240, pp. 1217
DOI: 10.1126/science.aaa8299

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Reid and Nicchitta propose that most cellular translation is carried out by a noncycling pool of endoplasmic reticulum (ER)–associated ribosomes. However, proximity-specific ribosome profiling data place an upper bound of about 7 to 16% on the fraction of cytosolic protein translation carried out by ribosomes accessible to ER-tethered biotin ligases. Moreover, yeast pulse-labeling experiments argue against there being a static population of ER-associated ribosomes.

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