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Science  26 Jun 2015:
Vol. 348, Issue 6242, pp. 1493
DOI: 10.1126/science.348.6242.1493-a

Automated Western Blot Processing

Based on proprietary sequential lateral flow (SLF), the new iBind Flex Western System is designed to enable a more versatile walk-away solution for the immunodetection step in a Western blotting workflow. Researchers now have the ability to adapt the iBind Flex Western Device to a variety of blot formats and sample throughput. Compatible with downstream chemiluminescent, colorimetric, or fluorescent detection protocols and optimized for higher sensitivity and reproducibility, there is potential for a significant reduction in primary antibody required when compared with manual blot processing. Experimental results can be achieved without the need for power, pumps, or vacuums through the use of SLF technology, which is designed to automate the blocking, primary and secondary antibody binding, and washing steps for immunodetection of proteins transferred to a nitrocellulose or PVDF membrane. The sequential and uniform flow of solutions across a glass fiber matrix ensures a consistent antigen-antibody interaction to deliver robust protein detection.

Host Cell Protein Detection and Quantification

The Gyrolab CHO-HCP Kit 1 detects and quantifies host cell proteins (HCP) from Chinese hamster ovary (CHO) cells used in bioprocessing of biotherapeutics. HCP are measured throughout the production process, as they may affect the safety and efficacy of the biotherapeutic. Gyrolab CHO-HCP Kit 1 has been validated for use on Gyrolab xP workstation and the new Gyrolab xPlore and is ready to use, eliminating the need for time-consuming assay development. The automation capabilities of Gyrolab systems enable 96 data points to be generated in approximately one hour without manual intervention, saving time, and reducing errors and repeats. The kit broadens the analytical range from typically two orders of magnitude to four, and therefore requires fewer dilutions and fewer reruns than alternative methods. Gyrolab CHO-HCP Kit 1 contains all the reagents needed to produce 96 data points, including a specific Bioaffy CD that has been optimized for this HCP assay.

IHC Reference Standards

A unique range of genetically defined, highly characterized HDx Reference Standards are now available for immunohistochemistry (IHC) assays. The introduction of IHC HDx Reference Standards provides histopathology laboratories with a consistent and reproducible source of reference material for the development and quality control of IHC assays. The independent external controls contain precisely defined protein expression levels presented on one slide, providing scientists the ability to easily determine the sensitivity of their assay. These standards are unique in having undergone a high level of characterization at a molecular and protein level, including Quantitative Digital Pathology, to quantify the intensity of staining. Variability in the quality of clinical samples and immunohistochemistry staining is a major concern within pathology laboratories as these techniques are used to confirm diagnoses. The use of independent external controls enables consistency, reproducibility, and accuracy to be maintained and monitored across the laboratory workflow, including operators, assays, manufacturers, and platforms.

Protein Interactions Assay

NanoBRET Protein Interaction Assays use a new Bioluminescence Resonance Energy Transfer (BRET) technology that enables scientists to quantitatively measure protein:protein interactions in live cells. With NanoBRET Protein Interaction Assays, researchers can study both induction and inhibition of protein interactions in real time using full-length proteins expressed at physiologically relevant levels. Conventional BRET measures the interaction of proteins using a bioluminescent donor fused to a protein of interest and a fluorescent acceptor fused to its binding partner; the donor does not excite the fluorophore using light, but transfers resonance energy through dipole-dipole coupling. The optimized NanoBRET Protein Interaction Assays use NanoLuc Luciferase as the energy donor and HaloTag protein as the energy acceptor. NanoBRET Technology has improved spectral overlap, increased signal, and provided lowered background. In addition, the brighter light output from NanoLuc enables use of NanoBRET even at low expression levels, while still providing efficient energy transfer.

Gel Imager

The easy-to-use PXi Access systems offer major advances on film detection and can rapidly generate high-quality images of virtually all types of large blots and gels. The new compact PXi 6 or 9 Access systems are complete with high-performance cameras capable of imaging a wide range of blot and gel types and sizes. The camera in each PXi Access features superb optics, which means scientists can set up quickly and detect even the faintest amounts of DNA or protein with a much greater sensitivity than film. PXi Access is so flexible that the systems can image stain-free gels and blots or those stained with any commercial chemiluminescence, fluorescence, visible, and IR dyes. For imaging chemi blots, the PXi Access uses a wide dynamic range to optimize exposure for each protein, accurately quantifying abundant and poorly expressed proteins, making this a much simpler method than using film for producing Western blot results.

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