Science Webinar Series

Improving tissue-sample profiling: The optimization and application of immunohistochemistry

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Science  03 Jul 2015:
Vol. 349, Issue 6243, pp. 104
DOI: 10.1126/science.349.6243.104-c

Summary

Though it has been used for more than 70 years, immunohistochemistry (IHC) is still an essential research and diagnostic tool in many scientific laboratories. Understanding the basic principles underlying IHC and how to address the technical aspects of experimental design are key to producing high-quality, reproducible data. IHC is used in a variety of fields—from cancer diagnostics to neuroscience research—but some common advice can be applied across-the-board. Many variables are vital for generating valuable results and require optimization when designing IHC experiments, such as fixing tissue, choosing the proper antibodies, and defining the proper controls. In this webinar, we will hear from experts who will share their insights into the key aspects of assay design.