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Science  07 Aug 2015:
Vol. 349, Issue 6248, pp. 656
DOI: 10.1126/science.349.6248.656-a

Live Cell Imager and Counter

The JuLI FL is the next generation, smart fluorescence live cell movie analyzer and counter. The JuLI FL uses novel optics to capture live-cell images from various cell culture dishes. It can perform cell-based assays, including cell counting, cell viability, and GFP/RFP expression level checking. This gives the user quantified cell confluence results with low variation and growth curve data. The JuLI features time-lapse image capturing and movie making for cell counting with GFP or RFP expression level checking. It can be used as a single unit or with a dual system to obtain comparative data. The easy-to-operate system features a user friendly interface with LCD touchscreen. Its compact size means that it fits any bench or can be placed inside a cell culture incubator with ease. Simple steps guide the user at each stage of the system setup, image capture, and the analysis operations.

Cell Energy Phenotype Test

The XFp Cell Energy Phenotype Test Kit enables a unique real-time assay on live cells that determines their baseline metabolic phenotype and potential. This 1-hour test measures both the mitochondrial and glycolytic activity of the cells, and compares their baseline values with metabolic activity under stressed conditions, induced by a single injection, to determine the metabolic potential—the cells' ability to respond to an energy demand. This kit has been designed specifically for use with the XFp Extracellular Flux Analyzer. The new XFp Cell Energy Phenotype Test is the only method available that can provide a metabolic phenotype with which scientists can make direct, functional comparisons of both metabolic pathways between groups of live cells. With this information cancer researchers can then quickly realize the functional consequences of somatic mutations in terms of metabolic adaptations and reprogramming events that drive tumor malignancies.

Cell Culture Growth Supplement

A new GroPro Cell Culture Growth Supplement allows for culturing of primary cells and cell lines without the concern for transmission of bovine prions and xenogeneic reactions. The new GroPro line delivers higher standards of purity, more affordable cell culture solutions in biomedical research. This new GroPro supplement is a convenient, ready-to-use human platelet lysate (HPL) formulation that can be used at the same supplementation percentages as fetal bovine serum (FBS) in traditional cultures. HPL has proven to be an excellent cost effective substitute for FBS in cell culture growth medium without the risk of xenogeneic infections or immune reactions. Currently, the use of HPL in culture medium requires the addition of heparin to prevent alloimmune aggregation which can create visible clots in the medium. The GroPro product line does not require the use of heparin thus reducing deleterious downstream effects, yet formulated to be used at normal FBS concentrations.

Stem Cell Media

A new pluripotent stem cell (PSC) medium is designed to offer researchers unprecedented schedule flexibility, allowing 2-day breaks from culture feeding without having to make changes to current workflows or compromise culture quality. Many stem cell media components, such as fibroblast growth factor 2 (FGF2), lose activity at physiologically relevant temperatures and, therefore, stem cell cultures must be fed daily to preserve pluripotency and tri-lineage differentiation potential. To achieve this, scientists typically must work through the weekend or delegate feeding steps to other scientists, which can introduce unnecessary variability. With this optimized formulation, Essential 8 Flex Medium grows and maintains PSCs over an extended length of time. Unlike other PSC culture solutions that promise, "weekend-free feeding," the new Gibco medium does not require substantial split-ratio adjustments prior to the break in feeding. Moreover, Essential 8 Flex Medium retains nearly 90% FGF2 activity after 72 hours.

Reprogramming Bundle

The L7 PBMC Reprogramming Bundle is a robust, streamlined and comprehensive platform for the generation of human induced pluripotent stem cells (hiPSCs) and the maintenance of stem cells from a variety of sources. This new bundle slots seamlessly into the existing L7 workflow to enable researchers to reprogram peripheral blood mononuclear cells (PBMCs) for use in downstream experiments. The L7 hPSC System offers a complete workflow that combines primary cells, reprogramming kits, transfection tools, and culturing media, along with a matrix, passaging solution, and cryosolution. As such, it streamlines the reprogramming of somatic cells to generate hiPSCs, as well as supporting the ongoing maintenance and utilization of human embryonic stem cells (hESCs) and hiPSCs. hiPSCs created using the new L7 PBMC Reprogramming Bundle can efficiently differentiate into all three germ layers and are ready for use across a wide range of applications, from basic research and disease modeling, through to drug development and regenerative medicine.

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