Drug Screening

Imaging to improve drug target mapping

See allHide authors and affiliations

Science  05 Feb 2016:
Vol. 351, Issue 6273, pp. 572-573
DOI: 10.1126/science.351.6273.572-c

Finding effective new drugs, or combinations thereof, that are free of toxicity and off-target effects is a big challenge—one that may only be solved with large amounts of data. Breinig et al. present a screening method that includes automated quantitative high-throughput imaging to track not only cell viability and proliferation, but also other features such as DNA texture, nuclear and cell shape, and cytoskeletal properties. Screening over 1200 pharmacological agents in 12 isogenic cell lines allowed the mapping of quantitative “footprints” or signatures of phenotypic responses to a drug (the data with over 300,000 drug-gene-phenotype interactions are available at an interactive webpage: http://dedomena.embl.de/PGPC/). The authors found interactions between inhibitors of MEK protein kinases and an antialcoholism drug and between a receptor tyrosine kinase inhibitor and the proteasome.

Mol. Syst. Biol. 10.15252/msb.20156400 (2015).

Navigate This Article