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Science  01 Apr 2016:
Vol. 352, Issue 6281, pp. 104
DOI: 10.1126/science.352.6281.104

XUV Spectrometer

McPherson's new grazing incidence wavelength dispersive optical spectrometer allows rapid analysis of spectral light in the ~1-nm to 300-nm wavelength region (4 eV to 1,200 eV.) In addition to performing high-harmonic laser generation experiments, the Model 248/310 is also useful for measuring ion- or discharge light sources and for astrophysics detector calibration. At FLASH, the free electron laser experiment conducted by DESY in Hamburg, Germany, the McPherson 248/310 ultrahigh vacuum (UHV) model assisted in analysis of the 2.3-nm to 4.4-nm water window (a region transparent to light). Space scientists and plasma physicists who need an instrument for vacuum ultraviolet and extreme ultraviolet (EUV/XUV) capitalize on the 248/310's range and flexibility. Configured in reverse, it operates as a tunable light source. This is useful for spectral calibration and spectroscopy in the vacuum ultraviolet and the extreme and soft X-ray wavelength region (EUV and SXR respectively). Direct detection charge-coupled devices (CCDs), image intensifiers, microchannel plates, or electron multipliers provide wide range and flexible detection.

qPCR Assays

The PrimeTime Gene Expression Master Mix is an optimized enzyme mix for probe-based quantitative polymerase chain reaction (qPCR). This newest product enhances the Integrated DNA Technologies (IDT) PrimeTime qPCR product line, as researchers can economically obtain both assays and a master mix that are optimized and validated to work together. PrimeTime qPCR Assays are available with a choice of dyes and quenchers, and the predesigned assays are backed by a performance guarantee. The versatile, stable master mix easily integrates into new and existing experimental workflows, because it is also compatible with all commercially available 5' nuclease gene expression assays. The PrimeTime qPCR Assays and Master Mix deliver accurate, sensitive gene expression analysis with efficiencies >90% in two-step reverse transcription (RT)-qPCR under both fast and standard cycling conditions. This provides the reproducibility that gives researchers confidence in the quality of their data, whether it be from singleplex or duplex reactions.

HPLC Column

The Thermo Scientific Acclaim AmG C18 high-performance liquid chromatography (HPLC) column is a high-performance, reversed-phase column designed for ion-pairing chromatographic separations and optimized for various aminoglycoside (AmG) antibiotics analyses, including drug purity and impurity characterization and quantification, therapeutic drug monitoring, and residual control testing in different matrices. AmG analysis requires columns that are robust to withstand harsh, low-pH conditions. The new Acclaim AmG C18 columns have been engineered specifically for this application. Our unique silica surface chemistry provides a layer of protection during the separation process and extends column longevity.

Microscope

Streamlining the cell-culture workflow in a range of application areas, Olympus' renowned optical expertise has given rise to the new CKX53 microscope, offering a novel inversion contrast (IVC) imaging technique. While a variety of capabilities enable the CKX53 to deliver high-quality images over a wide field of view for live-cell and fluorescence imaging, the system is also tailored to the cell-culture process with optimized ergonomics and fast, intuitive operation. Fast processing is vital to minimize the time precious live cells are removed from optimal incubation conditions, and Olympus' unique integrated Phase Contrast (iPC) system simplifies and speeds up phase contrast observations with the CKX53, removing the need to prepare or center the objectives when moving between 4X and 40X magnification. Phase contrast observations are also enhanced with the new IVC technique, enabling 3D views devoid of halos or directional shadows.

Benchtop Workstation

The CleneCab Workstation is a low-cost, benchtop alternative to a cleanroom. For those who wish to create a local cleanroom or safe environment to prevent contamination when working with samples, this is the ideal solution. The CleneCab provides a bench top size environment of 62 × 42 × 77 cm. Constructed from 10-mm acrylic glass, the workstation protects the sample from external contamination and the user from biohazards. It can also be used for other situations in which a high demand for cleanliness or protection is required, as with radioactive labeling of DNA. Its construction provides ideal shielding from radioactive substances like 32P. Five powerful, timer-controlled ultraviolet (UV) tubes combined with special reflectors efficiently irradiate the entire working surface. The workstation is also equipped with a microprocessor to control the energy of the UV tubes. The UV dose can be preset very easily in energy (mJ) or time units (s).

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  • Newly offered instrumentation, apparatus, and laboratory materials of interest to researchers in all disciplines in academic, industrial, and governmental organizations are featured in this space. Emphasis is given to purpose, chief characteristics, and availabilty of products and materials. Endorsement by Science or AAAS of any products or materials mentioned is not implied. Additional information may be obtained from the manufacturer or supplier.

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