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Science  27 May 2016:
Vol. 352, Issue 6289, pp. 1128
DOI: 10.1126/science.352.6289.1128-a

Chemiluminescence Detection

The Omega Lum C is optimized for the superior detection of chemiluminescent Western blots, fluorescence imaging such as DNA gels, blue light, and visible light applications. It is compact, affordable, and comes with all you need for the complete imaging package, including a camera with over 8 megapixels of resolution, cooled to –50°C, and a high-quality f0.95 lens. Add to this a wide dynamic range and fast imaging speeds, and you will spend less time while capturing high-quality images. The system provides the high levels of sensitivity, broad dynamic range, and accuracy required to perform quantitative Western blotting. Preloaded protocols enable the user to quickly access the rapid automatic functions of the system through its SmartCapture technology. The system contains a dual-wavelength transilluminator and an integrated tablet PC. The Omega Lum C has one of the smallest footprints for this type of product on the market.

Field-Flow Fractionation

The Postnova AF2000 MultiFlow is a high-performance, flow field-flow fractionation (FFF) platform for separation of proteins, macromolecules, and nanoparticles. Incorporating a range of FFF modules in a single integrated system to provide universal separation, the AF2000 MultiFlow offers more flexibility, better performance, and more robust results than any system previous to it. It uses a unique crossflow field design in which samples are separated by their dynamic diffusion on the basis of molar mass or particle size. Because of this design and the absence of any stationary phase, FFF on the AF2000 MultiFlow can be performed without exerting shear forces and stress on the molecules or particles being separated. The AF2000 can be run with different eluents and wide-ranging temperature conditions, and the same system can be used for running planar Asymmetric Flow FFF or circular Hollow Fiber Flow FFF separations. Consequently it is an ideal platform for the characterization of proteins, antibodies, aggregates, vaccines, virus-like particles (VLPs), liposomes, nanoparticles, and natural or synthetic polymers.

Mass Spectrometer

Advion recently announced the introduction of a proteins and peptides analysis system, 'TIDES EXPRESS. The system combines a new, easy-to-use, high-performance liquid chromatography (HPLC) system with the extended mass range expression L compact mass spectrometer (CMS). The new platform improves the workflow for chemists performing peptide synthesis and needing fast mass analysis of each reaction step as well as final products. It is the perfect technique for monitoring reactions in solid-phase peptide synthesizers. Additional fragmentation analysis and charge deconvolution tools enhance its use for protein characterization. Solid-phase peptide synthesis (SPPS) allows for rapid, parallel synthesis of peptides of various lengths. Mass spectrometry provides both molecular mass and amino acid sequence information through specific and selective molecule fragmentation along the amino acid backbone. The 'TIDES EXPRESS includes an easy-to-use HPLC gradient system with a manual sample injector and expression L CMS, as well as fragmentation analysis and charge deconvolution software tools for easy and excellent data interpretation.

3D Structure-Based Design

Optibrium has launched version 6.3 of its StarDrop software platform, which includes a new module providing seamless access to best-in-class, structure-based design technologies, based on BioSolveIT's SeeSAR package. The addition of intuitive visualization of 3-dimensional (3D) protein-ligand interactions to StarDrop results in a fully integrated environment for analysis and visualization of compound data, coupled with a comprehensive range of ligand- and structure-based design capabilities that guide the optimization of high-quality compounds. The new SeeSAR module for StarDrop provides a state-of-the-art, scientifically rigorous approach to understanding the binding of compounds in their protein targets in 3D. Users can import ligand and protein structures derived from crystal structures or predicted with any docking software, and visualize the key interactions driving potency. This is seamlessly linked to StarDrop's chemoinformatics methods based on 2D-compound structure and its unique Card View approach to interpreting the resulting structure-activity relationships.

Streptavidin Microcolumns

The new MSIA Streptavidin EVO microcolumns have been designed to bring the novel mass spectrometric immunoassay (MSIA) affinity purification technology to Tecan's Freedom EVO series of robotic platforms that are equipped with a 96-multichannel arm (MCA96). When combined with the Tecan Freedom EVO robotic platform, MSIA Streptavidin EVO microcolumns enable fast, accurate isolation of target analytes from complex biological matrices. Housed within a pipette tip, the proprietary monolithic columns are densely coated with streptavidin for analytical affinity purification of any biotinylated affinity ligand, even at low concentration. Because this technology is incorporated into a pipette tip, users can analyze sample volumes as low as 10 µL, saving precious samples. Furthermore, when compared to traditional resin- and bead-based methods, MSIA technology provides lower background noise and improved analytical sensitivity, while the simple workflow enables easier method standardization and transfer between labs.

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  • Newly offered instrumentation, apparatus, and laboratory materials of interest to researchers in all disciplines in academic, industrial, and governmental organizations are featured in this space. Emphasis is given to purpose, chief characteristics, and availabilty of products and materials. Endorsement by Science or AAAS of any products or materials mentioned is not implied. Additional information may be obtained from the manufacturer or supplier.

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