Cell Biology

Exploring the human proteome

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Science  24 Jun 2016:
Vol. 352, Issue 6293, pp. 1531-1532
DOI: 10.1126/science.352.6293.1531-e

Although we know the sequence of the human genome, a large fraction of the human proteome remains poorly characterized. To overcome this, Leonetti et al. combine two methods to achieve high-throughput tagging of human proteins. First, they use the CRISPR/Cas9 system to insert DNA that includes a sequence encoding the tag, a short piece of green fluorescent protein (GFP), as well as sequences homologous to targeted genes. Second, they coexpress the remaining part of GFP in the same cell, which binds to the tag to give fluorescent GFP. Such tagging will probably provide new insights into the functions of proteins and pave the way for proteome-wide analysis of human cells.

Proc. Natl. Acad. Sci. U.S.A. 10.1073/pnas.1606731113 (2016).

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