Science Webinar Series

Webinar | Combating the "garbage-in-garbage-out" paradigm: The importance of library prep in next-gen sequencing workflows

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Science  23 Sep 2016:
Vol. 353, Issue 6306, pp. 1449
DOI: 10.1126/science.353.6306.1449-b

Summary

In the context of next-generation sequencing, the old saying "garbage in, garbage out" translates to "poor sample preparation results in poor data"—sometimes even unusable data. As those poor results often become apparent only after expensive, time-consuming sequencing, it behooves researchers to pay extra attention to the preparation of their DNA samples at every step, from isolation through library preparation to loading the samples onto their sequencer of choice. This webinar will focus particularly on best practices for library preparation, including whole-genome library prep and shearing methods for PCR-free vs. PCR-plus prep, which can substantially impact bias and coverage, among other variables. Attendees will also hear about best practices for quality control of the library preparation process, as well as the impact of library quality on downstream data quality.