Medical Diagnostics

Fast point-of-care detection of biomarkers

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Science  13 Jan 2017:
Vol. 355, Issue 6321, pp. 144
DOI: 10.1126/science.355.6321.144-c

Many diseases can be diagnosed by detecting nucleic acid or protein biomarkers, but, with few exceptions, this detection requires complex and costly instruments. Du et al. adapted commercially available pregnancy kits for affordable and fast point-of-care diagnostics. In the detection method, nucleic acid is first amplified and then captured by a DNA oligonucleotide–human chorionic gonadotropin conjugate. The latter is detected by the commercial kits. The method allowed detection of just 20 copies of an Ebola virus template and could distinguish a melanoma-related biomarker from the wild-type sequence. It should be possible to adapt it for low-cost detection of other biomarkers in clinical settings.

Angew. Chem. Int. Ed. Engl. 10.1002/anie.201609108 2016).

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