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Assembly of a nucleus-like structure during viral replication in bacteria

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Science  13 Jan 2017:
Vol. 355, Issue 6321, pp. 194-197
DOI: 10.1126/science.aal2130

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Phages build themselves a wall

The compartmentalization of DNA replication away from other cytoplasmic events is a key feature of the cell nucleus. Chaikeeratisak et al. studied the replication of the very large Pseudomonas bacteriophage 201φ2-1 by using fluorescence microscopy and cryo–electron tomography. They found that the phage assembled a nucleus-like compartment when it infected a bacterial cell. The phage genome was completely enclosed by an apparently contiguous protein shell, within which DNA replication, recombination, and transcription occurred. Translation, precursor biosynthesis, and viral assembly occurred outside the structure.

Science, this issue p. 194

Abstract

We observed the assembly of a nucleus-like structure in bacteria during viral infection. Using fluorescence microscopy and cryo-electron tomography, we showed that Pseudomonas chlororaphis phage 201φ2-1 assembled a compartment that separated viral DNA from the cytoplasm. The phage compartment was centered by a bipolar tubulin-based spindle, and it segregated phage and bacterial proteins according to function. Proteins involved in DNA replication and transcription localized inside the compartment, whereas proteins involved in translation and nucleotide synthesis localized outside. Later during infection, viral capsids assembled on the cytoplasmic membrane and moved to the surface of the compartment for DNA packaging. Ultimately, viral particles were released from the compartment and the cell lysed. These results demonstrate that phages have evolved a specialized structure to compartmentalize viral replication.

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