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Science  13 Jan 2017:
Vol. 355, Issue 6321, pp. 210
DOI: 10.1126/science.355.6321.210-a

CRISPR RNA Arrayed Library

The Dharmacon Edit-R Human Druggable Genome crRNA Library enables screening of nearly 8,000 individual targets with CRISPR/Cas9 gene knockouts. The first arrayed synthetic CRISPR RNA (crRNA) library of its kind, it provides insight into numerous biological questions and offers a powerful screening resource to identify potential therapeutic targets. The Edit-R crRNA Library delivers one-gene-per-well information by enabling high content and multiparametric assays to characterize complex phenotypes. Ready-to-use, transfectable Edit-R synthetic crRNAs are designed using an algorithm that results in highly functional gene knockout, while also accurately identifying and eliminating sequences with the potential for off-target editing. The library offers 7,995 gene targets with four crRNAs per gene; 96- and 384-well-plate formats with quantities of 0.1 nanomole (nmol), 0.25 nmol, or 0.5 nmol per well; and subsets arranged by gene family including kinases, proteases, phosphatases, ion channels, transcription factors, G-protein–coupled receptors, ubiquitin enzymes, and other potential drug targets.

CRISPR gRNA Lentivector Cloning Kits

CRISPR gRNA Lentivector Cloning Kits from AMS Biotechnology provide targeted and precise gene editing methodology. The recently discovered CRISPR/Cas gene editing technique offers higher targeting accuracy, more target-sequence selection, less complexity, and less off-target cell toxicity than previous genome editing technologies. To enable CRISPR genomic editing, the kit provides premade Cas9-expression lentivirus, as well as guide RNA (gRNA) lentivector cloning kits from which researchers can construct their target gRNA lentivectors. Applying both Cas9 lentivirus and gRNA lentivirus allows target knockout or modification (knockin) when a donor DNA is also applied. The kits enable rapid, precise directional cloning of DNA duplex-encoded gRNA structure at efficiencies of greater than 95%. The lentivector included in each kit encodes an antibiotic marker or a dual marker (a fluorescent-antibiotic fusion marker), enabling generation of stable cell lines for long-term gRNA expression. Two promoters are available: U6 and an optional inducible H1 promoter.

Genomic Data Analysis Sofware

Illumina's NGS-based CytoSeq assay extends beyond traditional microarray-based cytogenetics assays for detection of copy number variation (CNV) and absence of heterozygosity (AOH), but also adds the ability to identify single nucleotide variation, all in a single assay. Coupled with BioDiscovery's NxClinical software for analysis and interpretation of genomic events, this new solution will offer customers a powerful and cost-effective research tool for studying CNVs, AOH, and mutations in genetic disorders. NxClinical software will be offered with the CytoSeq assay, providing an easy-to-use, interactive instrument for visualization and interpretation of genomic events, directly reading CytoSeq results to process sample data and provide results.

Gene-Edited Cell Lines

Horizon's TurboGFP tagged HAP1 cell lines enable researchers to track protein production and localization at the endogenous level in real time, live-cell assays. The cells combine three leading technologies: CRISPR/Cas9 gene editing; the HAP1 (Huntingtin-associated protein 1) cell line; and TurboGFP, an early-maturation green fluorescent protein (GFP) from Evrogen. Although gene editing technologies are now readily available, the processes are still not straightforward, and can be costly in terms of time and resources. All of the award-winning products in Horizon's portfolio have been designed to provide researchers with access to the latest technologies, effectively democratizing gene editing. These cell lines are also suitable for applications such as Western blotting, protein pulldown, affinity chromatography, immunocytochemistry, and flow cytometry. They can also be an ideal solution when working with a protein for which there is no optimal antibody.

CRISPR Guide Libraries

Agilent Technologies offers pooled CRISPR guide libraries for any functional genomics application, from genome-scale CRISPR knockouts (GeCKO) to fully customized, user-designed libraries. Genome engineering tools based on CRISPR have rapidly accelerated research related to functional studies of complex diseases and drug discovery. Genetic screens using pooled libraries are typically performed to locate and identify genes involved in cellular response, such as in signaling pathways, or to discover the function of novel genes. SureGuide CRISPR libraries are available in three formats: (1) ready-to-package plasmid libraries that consist of GeCKO libraries and target all exons in the human or mouse genome; (2) ready-to-clone custom libraries that enable user-defined custom CRISPR targets in mammalian cells; and (3) ready-to-amplify custom libraries that enable researchers to design every aspect of their CRISPR library, allowing the use of alternative delivery systems, cloning approaches, and the development of CRISPR libraries targeting any set of genomic regions in any organism.

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