Pushing the limits of functional genomics

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Science  27 Jan 2017:
Vol. 355, Issue 6323, pp. 364-365
DOI: 10.1126/science.355.6323.364-f

CRISPR-based technology is making the analysis of complex phenotypes possible on a large scale. Adamson et al. and Dixit et al. present methods to analyze the effects of multiple perturbations on gene expression. They combined CRISPR-mediated partial or complete deletion of multiple gene products with single-cell RNA sequencing, with the latter identifying each particular cell by a “barcode” delivered with the CRISPR vector. Computational analyses allowed clustering of genes and characterization of processes and state s in individual cells. The responses of mouse immune cells to manipulation of 24 transcription factors alone and in combination, as well as that of human cells to perturbation of the 100 genes implicated in the unfolded protein response, show that the methods can help clarify complex regulatory programs in cells.

Cell 10.1016/j.cell.2016.11.048, 10.1016/j.cell.2016.11.038 (2016).

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