Editors' ChoiceGenetics

Manipulating the fly conditionally

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Science  31 Mar 2017:
Vol. 355, Issue 6332, pp. 1387-1388
DOI: 10.1126/science.355.6332.1387-d

The fruit fly Drosophila has been indispensable in the genetics and biochemical toolkits. This model organism has enabled many advances in understanding early development, metabolism, cell biology, evolution, and so much more. However, most knockout methods fail to completely eliminate gene function and do not work in differentiated cells. Fisher et al. developed “FlpStop” for conditional gene disruption and gene rescue. They showed proof of principle by generating conditional null alleles for the wing development gene apterous. Follow-up analyses manipulated genes involved in GABAergic neurotransmission or neuronal excitability within different cell types or cellular compartments. The tool can now be applied to other differentiated or undifferentiated cells at specific times of development to better understand the interactions between genes and within genetic networks.

eLife 10.7554/eLife.22279.001 (2017).

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