Science Webinar Series

Webinar | Monitoring immune function by imaging flow cytometry

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Science  02 Jun 2017:
Vol. 356, Issue 6341, pp. 973
DOI: 10.1126/science.356.6341.973-b

Summary

The immune system plays a critical role not only in fending off pathogen attack, but also in cancer surveillance, and more recently as a tool in immunotherapy-based treatments. Immune cell functions are tightly regulated by essential transcription factors such as NF-κB and NFAT. Monitoring immune cell activity—including phenotyping immune cell subsets, tracking cell proliferation, and measuring cytokine production—can provide insights into the overall status of immune function in patients, particularly those undergoing immunosuppression after transplants, enduring cancer treatment, or suffering from autoimmune disease or other pathologies that affect the immune system. Imaging flow cytometry (IFC) has emerged as a useful and efficient tool for studying the signaling pathways in immunophenotypically defined subpopulations of immune cells. This technique enables quantitative image analysis of the intracellular localization of the signaling intermediaries NF-κB and NFAT as parameters of immune activity. This webinar will introduce viewers to the process of using IFC to determine subcellular localization of biomarkers, including a discussion of how IFC can help to assess the activity of transcription factors, or the drug-induced stimulation or inhibition thereof, in clinical samples.