Science Webinar Series

Webinar | CRISPR unleashed: New tools and applications in live-cell imaging

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Science  24 Nov 2017:
Vol. 358, Issue 6366, pp. 1088
DOI: 10.1126/science.358.6366.1088-b

Summary

The CRISPR/Cas9 gene editing system has been a boon for researchers, enabling them to manipulate a broad range of genomes quickly and accurately. This novel, versatile tool has been used with great precision for DNA editing as well as a multitude of other applications. Recent enhancements have expanded its abilities; one example is a new, hyperaccurate Cas9 variant that demonstrates high specificity across the genome without compromising on-target activity in human cells. Another is a modification of Cas9 called CRISPRainbow, a system that can more easily label multiple genomic loci in living cells, allowing researchers to better study chromatin dynamics. The unprecedented flexibility of the CRISPR toolset makes it possible to capture imaging phenomena happening at very small scales of space and time. An essential piece of this technology is a camera that captures the fine structure necessary to see the modified DNA (and distinguish it from the background). It must be capable of functioning in challenging environments in living cells, which require cutting-edge light manipulation and detection technologies optimized for light-starved conditions. During this webinar, our experts will examine some of the latest enhancements to the CRISPR/Cas9 system and explain how they are being applied in the lab today.